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PacBio-Puv-ntev 16S/18S/ITS Amplicon Sequencing
Amplicon (16S/18S/ITS) platform tau tsim los nrog ntau xyoo ntawm kev paub dhau los hauv kev tshuaj xyuas qhov project microbial diversity, uas muaj cov qauv kev tshuaj xyuas yooj yim thiab kev tshuaj xyuas tus kheej: kev tshuaj xyuas yooj yim npog cov ntsiab lus tshuaj xyuas tseem ceeb ntawm kev tshawb fawb microbial tam sim no, cov ntsiab lus tshuaj xyuas yog nplua nuj thiab dav dav, thiab cov txiaj ntsig tshuaj xyuas tau nthuav tawm hauv daim ntawv ntawm cov ntawv qhia project; Cov ntsiab lus ntawm kev tshuaj xyuas tus kheej yog ntau haiv neeg. Cov qauv tuaj yeem xaiv thiab cov kev teeb tsa tuaj yeem teeb tsa tau yooj yim raws li daim ntawv qhia kev tshuaj xyuas yooj yim thiab lub hom phiaj tshawb fawb, kom ua tiav cov kev xav tau tus kheej. Windows operating system, yooj yim thiab ceev.
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PacBio-Puv-ntev Transcriptome (Tsis Yog Siv)
Siv cov ntaub ntawv Pacific Biosciences (PacBio) Isoform sequencing ua cov tswv yim, lub App no muaj peev xwm txheeb xyuas cov kab lus sau tag nrho (tsis muaj kev sib dhos). Los ntawm kev kos cov kab lus tag nrho tawm tsam cov genome siv, cov ntawv sau tuaj yeem ua kom zoo dua los ntawm cov noob paub, cov ntawv sau, cov cheeb tsam coding, thiab lwm yam. Hauv qhov no, kev txheeb xyuas cov qauv mRNA kom raug dua, xws li lwm txoj hauv kev splicing, thiab lwm yam, tuaj yeem ua tiav. Kev tshuaj xyuas ua ke nrog cov ntaub ntawv NGS transcriptome sequencing ua rau muaj kev sau ntawv ntau dua thiab kev ntsuas ntau dua hauv kev hais tawm ntawm theem ntawv sau, uas feem ntau pab tau rau kev hais tawm sib txawv thiab kev tshuaj xyuas kev ua haujlwm.
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Cov Cuab Yeej Siv
BMKCloud yog ib lub platform bioinformatic uas muab kev daws teeb meem ib zaug rau cov kev pab cuam genomic, uas cov kws tshawb fawb ntseeg siab hauv ntau qhov chaw suav nrog kev kho mob, kev ua liaj ua teb, ib puag ncig, thiab lwm yam. BMKCloud cog lus tias yuav muab cov kev pabcuam sib xyaw, txhim khu kev qha thiab ua haujlwm tau zoo suav nrog cov platforms thiab cov cuab yeej tshuaj xyuas bioinformatic, cov peev txheej suav, cov ntaub ntawv pej xeem, cov chav kawm online bioinformatic, thiab lwm yam. BMKCloud muaj ntau yam cuab yeej bioinformatic siv ntau zaus suav nrog kev sau ntawv gene, cov cuab yeej hloov pauv genetic, ncRNA, kev tswj xyuas cov ntaub ntawv zoo, kev sib dhos, kev sib dhos, kev rho tawm cov ntaub ntawv, kev hloov pauv, kev suav lej, tus tsim duab, kev tshuaj xyuas kab ke, thiab lwm yam.
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RNA me me
Cov RNA me me yog hom RNA luv luv uas tsis yog coding nrog qhov ntev nruab nrab ntawm 18-30 nt, suav nrog miRNA, siRNA thiab piRNA. Cov RNA me me no tau raug tshaj tawm tias koom nrog ntau yam txheej txheem ntawm lub cev xws li kev puas tsuaj ntawm mRNA, kev txwv tsis pub txhais lus, kev tsim heterochromatin, thiab lwm yam. Kev tshuaj xyuas SmallRNA sequencing tau siv dav hauv kev tshawb fawb txog kev loj hlob ntawm tsiaj / tsob nroj, kab mob, kab mob, thiab lwm yam. Lub platform tshuaj xyuas RNA sequencing me me muaj kev tshuaj xyuas tus qauv thiab kev tshawb nrhiav cov ntaub ntawv siab heev. Raws li cov ntaub ntawv RNA-seq, kev tshuaj xyuas tus qauv tuaj yeem ua tiav kev txheeb xyuas thiab kwv yees miRNA, kev kwv yees cov noob caj noob ces miRNA, kev sau ntawv thiab kev tshuaj xyuas kev qhia tawm. Kev tshuaj xyuas siab heev ua rau muaj kev tshawb nrhiav thiab rho tawm miRNA, kev tsim daim duab Venn, miRNA thiab kev tsim cov tes hauj lwm noob caj noob ces.
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NGS-WGS (Illumina/BGI)
NGS-WGS yog ib lub platform tshuaj xyuas tag nrho cov noob caj noob ces (genome re-sequencing analysis platform), uas tau tsim los ntawm kev paub dhau los hauv Biomarker Technologies. Lub platform yooj yim siv no tso cai rau kev xa cov txheej txheem tshuaj xyuas sib xyaw ua ke sai sai los ntawm kev teeb tsa ob peb qhov yooj yim, uas haum rau cov ntaub ntawv DNA sequencing uas tsim los ntawm Illumina platform thiab BGI sequencing platform. Lub platform no tau xa mus rau ntawm lub server computing ua haujlwm siab, uas ua rau muaj kev tshuaj xyuas cov ntaub ntawv zoo heev hauv lub sijhawm luv luv. Kev tshawb nrhiav cov ntaub ntawv tus kheej muaj nyob rau ntawm kev tshuaj xyuas tus qauv, suav nrog kev nug cov noob caj noob ces hloov pauv, PCR primer tsim, thiab lwm yam.
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mRNA (Siv los ua piv txwv)
Transcriptome yog qhov txuas ntawm cov ntaub ntawv genomic genetic thiab proteome ntawm kev ua haujlwm ntawm lub cev. Kev tswj hwm theem transcriptional yog qhov tseem ceeb tshaj plaws thiab txoj kev tswj hwm uas tau kawm ntau tshaj plaws ntawm cov kab mob. Transcriptome sequencing tuaj yeem ua kab ke transcriptome txhua lub sijhawm lossis nyob rau hauv txhua qhov xwm txheej, nrog rau qhov kev daws teeb meem raug rau ib qho nucleotide. Nws tuaj yeem cuam tshuam txog qib ntawm kev sau ntawv gene, tib lub sijhawm txheeb xyuas thiab ntsuas cov ntawv sau tsis tshua muaj thiab ib txwm muaj, thiab muab cov ntaub ntawv qauv ntawm cov ntawv sau tshwj xeeb.
Tam sim no, cov thev naus laus zis transcriptome sequencing tau siv dav hauv kev ua liaj ua teb, tshuaj thiab lwm yam kev tshawb fawb, suav nrog kev tswj hwm kev loj hlob ntawm tsiaj thiab nroj tsuag, kev hloov pauv ib puag ncig, kev sib cuam tshuam ntawm lub cev tiv thaiv kab mob, kev nyob hauv cov noob caj noob ces, kev hloov pauv ntawm cov noob caj noob ces thiab kev kuaj pom kab mob qog nqaij hlav thiab noob caj noob ces.
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Kev Tshawb Fawb Txog Metagenomics (NGS)
Lub platform tshuaj xyuas no yog tsim los rau kev tshuaj xyuas cov ntaub ntawv metagenomic raws li ntau xyoo ntawm kev paub dhau los. Nws muaj cov txheej txheem ua haujlwm sib xyaw ua ke uas muaj ntau yam kev tshuaj xyuas metagenomics uas xav tau suav nrog kev ua cov ntaub ntawv, kev tshawb fawb txog hom tsiaj, kev tshawb fawb txog kev ua haujlwm ntawm cov noob caj noob ces, kev sib xyaw ua ke ntawm metagenome, thiab lwm yam. Tsis tas li ntawd, cov cuab yeej tshawb nrhiav cov ntaub ntawv tshwj xeeb muaj nyob rau ntawm cov txheej txheem tshuaj xyuas tus qauv, suav nrog kev nug txog noob caj noob ces thiab hom tsiaj, kev teeb tsa parameter, kev tsim cov duab tus kheej, thiab lwm yam.
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LncRNA
Cov RNA ntev uas tsis yog coding (lncRNA) yog ib hom transcripts uas ntev dua 200 nt, uas tsis muaj peev xwm code cov proteins. Cov pov thawj sib sau ua ke qhia tias feem ntau ntawm lncRNAs yuav ua haujlwm tau zoo. Cov thev naus laus zis high-throughput sequencing thiab cov cuab yeej bioinformatic analysing ua rau peb qhia tau lncRNA sequences thiab cov ntaub ntawv qhov chaw zoo dua thiab coj peb mus nrhiav lncRNAs nrog cov haujlwm tswj hwm tseem ceeb. BMKCloud zoo siab los muab peb cov neeg siv khoom lncRNA sequencing analysis platform kom ua tiav kev tshuaj xyuas lncRNA sai, txhim khu kev qha thiab yooj ywm.
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GWAS
Kev tshawb nrhiav txog kev sib raug zoo ntawm cov noob caj noob ces (GWAS) lub hom phiaj yog txheeb xyuas cov noob caj noob ces sib txawv (genotype) uas cuam tshuam nrog cov yam ntxwv tshwj xeeb (phenotype). Kev tshawb nrhiav GWA tshawb nrhiav cov cim noob caj noob ces hla tag nrho cov noob caj noob ces ntawm cov tib neeg coob coob thiab kwv yees kev sib raug zoo ntawm cov noob caj noob ces thiab cov yam ntxwv los ntawm kev tshuaj xyuas cov ntaub ntawv ntawm cov pej xeem. Kev rov ua dua tag nrho cov noob caj noob ces tuaj yeem nrhiav pom txhua qhov sib txawv ntawm cov noob caj noob ces. Kev sib txuas nrog cov ntaub ntawv phenotypic, GWAS tuaj yeem ua tiav los txheeb xyuas cov SNPs, QTLs thiab cov noob caj noob ces uas cuam tshuam nrog phenotype, uas txhawb nqa kev yug tsiaj/cog niaj hnub no. SLAF yog ib txoj kev tshawb nrhiav genome yooj yim uas tsim los ntawm tus kheej, uas tshawb pom cov cim qhia thoob plaws genome, SNP. Cov SNPs no, ua cov cim qhia noob caj noob ces molecular, tuaj yeem ua tiav rau kev tshawb nrhiav kev sib raug zoo nrog cov yam ntxwv tsom. Nws yog ib txoj kev pheej yig hauv kev txheeb xyuas cov yam ntxwv nyuaj uas cuam tshuam nrog kev hloov pauv noob caj noob ces.
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Nanopore Cov ntawv sau tag nrho ntev
Cov isoforms sib txawv thiab sib txawv hauv cov kab mob yog cov txheej txheem tseem ceeb rau kev tswj hwm kev qhia tawm ntawm cov noob caj noob ces thiab cov protein ntau yam. Kev txheeb xyuas qhov tseeb ntawm cov qauv sau ntawv yog lub hauv paus rau kev kawm tob txog cov qauv kev tswj hwm kev qhia tawm ntawm cov noob caj noob ces. Nanopore sequencing platform tau ua tiav coj kev kawm transcriptomic mus rau theem isoform. Lub platform tshuaj xyuas no yog tsim los tshuaj xyuas cov ntaub ntawv RNA-Seq tsim los ntawm Nanopore platform ntawm lub hauv paus ntawm cov genome siv, uas ua tiav kev tshuaj xyuas zoo thiab ntau yam hauv ob qho tib si theem noob caj noob ces thiab theem ntawv sau.
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circ-RNA
RNA ncig (circRNA) yog ib hom RNA uas tsis yog coding, uas nyuam qhuav pom tias ua lub luag haujlwm tseem ceeb hauv kev tswj hwm cov tes hauj lwm uas koom nrog kev tsim kho, kev tiv thaiv ib puag ncig, thiab lwm yam. Txawv ntawm cov linear RNA molecules, piv txwv li mRNA, lncRNA, 3′ thiab 5′ kawg ntawm circRNA tau koom ua ke los ua ib lub voj voog, uas cawm lawv ntawm kev zom ntawm exonuclease thiab ruaj khov dua li feem ntau ntawm linear RNA. CircRNA tau pom tias muaj ntau yam haujlwm hauv kev tswj hwm kev qhia tawm gene. CircRNA tuaj yeem ua haujlwm zoo li ceRNA, uas khi miRNA sib tw, hu ua miRNA daim txhuam cev. CircRNA sequencing analysis platform txhawb nqa circRNA qauv thiab kev qhia tawm, kev kwv yees lub hom phiaj thiab kev sib koom ua ke nrog lwm hom RNA molecules.
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BSA
Lub platform Bulked Segregant Analysis muaj ib kauj ruam kev tshuaj xyuas tus qauv thiab kev tshuaj xyuas siab heev nrog kev teeb tsa parameter. BSA yog ib txoj kev siv los txheeb xyuas cov cim qhia txog noob caj noob ces uas cuam tshuam nrog phenotype sai sai. Cov txheej txheem ua haujlwm tseem ceeb ntawm BSA muaj: 1. xaiv ob pawg neeg uas muaj cov phenotypes sib txawv heev; 2. sib sau ua ke DNA, RNA lossis SLAF-seq (Tsim los ntawm Biomarker) ntawm txhua tus neeg los ua ob pawg DNA; 3. txheeb xyuas cov kab ke sib txawv tawm tsam genome siv lossis nyob nruab nrab, 4. kwv yees cov cheeb tsam txuas nrog cov neeg sib tw los ntawm ED thiab SNP-index algorithm; 5. Kev tshuaj xyuas kev ua haujlwm thiab kev nplua nuj ntawm cov noob caj noob ces hauv cov cheeb tsam sib tw, thiab lwm yam. Kev khawb av ntau dua hauv cov ntaub ntawv suav nrog kev tshuaj xyuas cov cim qhia txog noob caj noob ces thiab kev tsim primer kuj muaj.
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Amplicon (16S/18S/ITS)
Amplicon (16S/18S/ITS) platform tau tsim los nrog ntau xyoo ntawm kev paub dhau los hauv kev tshuaj xyuas qhov project microbial diversity, uas muaj cov qauv kev tshuaj xyuas yooj yim thiab kev tshuaj xyuas tus kheej: kev tshuaj xyuas yooj yim npog cov ntsiab lus tshuaj xyuas tseem ceeb ntawm kev tshawb fawb microbial tam sim no, cov ntsiab lus tshuaj xyuas yog nplua nuj thiab dav dav, thiab cov txiaj ntsig tshuaj xyuas tau nthuav tawm hauv daim ntawv ntawm cov ntawv qhia project; Cov ntsiab lus ntawm kev tshuaj xyuas tus kheej yog ntau haiv neeg. Cov qauv tuaj yeem xaiv thiab cov kev teeb tsa tuaj yeem teeb tsa tau yooj yim raws li daim ntawv qhia kev tshuaj xyuas yooj yim thiab lub hom phiaj tshawb fawb, kom ua tiav cov kev xav tau tus kheej. Windows operating system, yooj yim thiab ceev.
