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Genome-wide Association Analysis
Genome-wide Association txoj kev tshawb fawb (GWAS) lub hom phiaj ntawm kev txheeb xyuas cov noob caj noob ces (genotype) uas cuam tshuam nrog cov yam ntxwv tshwj xeeb (phenotype).GWAS txoj kev tshawb fawb soj ntsuam cov cim caj ces hla tag nrho cov genome ntawm cov neeg coob coob thiab kwv yees cov koom haum genotype-phenotype los ntawm kev txheeb cais ntawm cov pej xeem.Nws tau siv dav hauv kev tshawb fawb txog tib neeg cov kab mob thiab kev ua haujlwm gene mining ntawm cov yam ntxwv ntawm cov tsiaj lossis cov nroj tsuag.
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Nroj / Tsiaj Tag Nrho Genome Sequencing
Tag nrho genome re-sequencing, tseem hu ua WGS, ua rau kev nthuav tawm ntawm ob qho tib si thiab tsis tshua muaj kev hloov pauv ntawm tag nrho cov genome suav nrog Ib Leeg Nucleotide Polymorphism (SNP), Insertion Deletion (InDel), Structure variation (SV), thiab Copy Number Variation (CNV). ).SVs ua ib feem loj ntawm qhov hloov pauv ntau dua li SNPs thiab muaj kev cuam tshuam ntau dua rau cov genome, uas muaj kev cuam tshuam loj rau cov kab mob nyob.Kev nyeem ntawv ntev ntev tso cai rau kev txheeb xyuas qhov tseeb ntawm cov kab mob loj thiab cov kev hloov pauv nyuaj vim kev nyeem ntev ua rau nws yooj yim dua rau chromosomal hla cov cheeb tsam nyuaj xws li tandem rov ua dua, GC / AT-nplua nuj cheeb tsam, thiab hyper-variable cheeb tsam.
Platform: Illumina, PacBio, Nanopore
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Evolutionary noob caj noob ces
Evolutionary noob caj noob ces yog ib qho kev pab cuam sib luag tsim los muab kev txhais lus dav dav ntawm cov ntaub ntawv hloov pauv ntawm cov khoom siv raws li kev hloov pauv caj ces, suav nrog SNPs, InDels, SVs thiab CNVs.Nws muab tag nrho cov kev txheeb xyuas tseem ceeb uas yuav tsum tau piav qhia txog cov kev hloov pauv hloov pauv thiab cov yam ntxwv ntawm cov noob caj noob ces, xws li cov qauv pej xeem, kev sib txawv ntawm caj ces, kev sib raug zoo ntawm phylogeny, thiab lwm yam.
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Sib piv Genomics
Kev sib piv genomics txhais tau hais tias sib piv cov genome ua tiav thiab cov qauv ntawm ntau hom.Qhov kev qhuab qhia no yog tsom rau kev nthuav tawm hom kev hloov pauv, kev ua haujlwm ntawm cov noob caj noob ces, cov txheej txheem tswj cov txheej txheem ntawm qib genome los ntawm kev txheeb xyuas cov qauv kab ke thiab cov ntsiab lus uas khaws cia lossis sib txawv ntawm ntau hom.Kev sib piv genomics txoj kev tshawb fawb suav nrog kev txheeb xyuas hauv tsev neeg gene, evolutionary txoj kev loj hlob, tag nrho genome duplication, xaiv lub siab, thiab lwm yam.
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Hi-C raws li Genome Assembly
Hi-C yog ib txoj hauv kev tsim los ntes chromosome configuration los ntawm kev sib txuas kev soj ntsuam raws li kev sib cuam tshuam thiab kev sib tw siab.Qhov kev siv ntawm cov kev sib cuam tshuam no ntseeg tau tias muaj kev cuam tshuam tsis zoo nrog lub cev nyob deb ntawm chromosomes.Yog li, Hi-C cov ntaub ntawv tuaj yeem coj cov pawg, kev txiav txim thiab kev taw qhia ntawm cov kab ke sib dhos hauv cov txheej txheem genome thiab khi rau cov uas mus rau qee qhov chromosomes.Cov thev naus laus zis no txhawb nqa lub chromosome-theem genome los ua ke thaum tsis muaj cov pej xeem raws li daim ntawv qhia caj ces.Txhua lub genome xav tau Hi-C.
Platform: Illumina NovaSeq Platform / DNBSEQ
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Nroj / Tsiaj De Novo Genome Sequencing
De Novosequencing yog hais txog kev tsim kho ntawm ib hom genome tag nrho siv cov thev naus laus zis sib txuas, xws li PacBio, Nanopore, NGS, thiab lwm yam, thaum tsis muaj kev siv genome.Qhov kev txhim kho zoo kawg nkaus hauv kev nyeem ntawv ntev ntawm peb tiam thib peb sequencing technologies tau coj lub cib fim tshiab hauv kev sib sau ua ke ntawm cov genomes, xws li cov neeg uas muaj heterozygosity siab, siab piv ntawm cov cheeb tsam rov ua dua, polyploids, thiab lwm yam. Nrog rau kev nyeem ntev ntawm kaum ntawm kilobases qib, cov kev sib txuas lus no tau pab. daws cov ntsiab lus rov ua dua, thaj chaw uas muaj cov ntsiab lus GC txawv txav thiab lwm thaj chaw nyuaj heev.
Platform: PacBio Sequel II / Nanopore PromethION P48 / Illumina NovaSeq Platform
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Human Whole Exome Sequencing
Tag nrho exome sequencing (WES) yog suav hais tias yog ib qho txiaj ntsim sequencing zoo rau kev txheeb xyuas cov kab mob uas ua rau muaj kev hloov pauv.Txawm hais tias exons tsuas yog siv li ntawm 1.7% ntawm tag nrho genome, nws sawv cev rau qhov profile ntawm tag nrho cov protein ua haujlwm ncaj qha.Hauv tib neeg genome, nws tau raug tshaj tawm tias ntau dua 85% ntawm cov kab mob cuam tshuam txog kev hloov pauv tshwm sim hauv cheeb tsam protein coding.
BMKGENE muab cov kev pabcuam dav dav thiab hloov tau yooj yim tib neeg tag nrho cov kev pabcuam exome sequencing nrog txawv exon capturing tswv yim muaj kom tau raws li ntau yam kev tshawb fawb lub hom phiaj.
Platform: Illumina NovaSeq Platform
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Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)
High-throughput genotyping, tshwj xeeb tshaj yog nyob rau hauv loj-scale pej xeem, yog ib tug tseem ceeb kauj ruam nyob rau hauv cov kev tshawb fawb ntawm genetic koom haum, uas muab genetic hauv paus rau functional gene discovery, evolutionary tsom xam, thiab lwm yam ) yog qhia kom txo qis tus nqi sequencing ib tus qauv, thaum tswj kev ua haujlwm tsim nyog ntawm kev tshawb nrhiav caj ces.Qhov no feem ntau ua tiav los ntawm kev rho tawm qhov txwv tsis pub dhau ntawm qhov loj me, uas yog lub npe txo qis cov tsev qiv ntawv (RRL).Specific-locus amplified fragment sequencing (SLAF-Seq) yog ib lub tswv yim tsim kho tus kheej rau SNP genotyping nrog lossis tsis muaj kev siv genome.
Platform: Illumina NovaSeq Platform
