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BSA
Bulked Segregant Analysis platform muaj cov txheej txheem kev soj ntsuam ib-kauj ruam thiab kev soj ntsuam siab heev nrog rau kev teeb tsa kev cai.BSA yog cov txheej txheem ua haujlwm sai sai txheeb xyuas cov phenotype txuam nrog cov cim caj ces.Lub luag haujlwm tseem ceeb ntawm BSA muaj: 1. xaiv ob pawg ntawm cov tib neeg uas muaj kev tawm tsam phenotypes;2. muab cov DNA, RNA lossis SLAF-seq (Tsim los ntawm Biomarker) ntawm txhua tus neeg los ua ob feem ntawm DNA;3. txheeb xyuas qhov sib txawv ntawm cov genome los yog nyob nruab nrab ntawm, 4. kwv yees cov neeg sib tw txuas cov cheeb tsam los ntawm ED thiab SNP-index algorithm;5. Kev soj ntsuam ua haujlwm thiab ua kom muaj txiaj ntsig zoo ntawm cov noob hauv cheeb tsam tus neeg sib tw, thiab lwm yam. Ntau cov mining siab heev hauv cov ntaub ntawv suav nrog kev tshuaj ntsuam genetic marker thiab primer design kuj muaj.
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Amplicon (16S/18S/ITS)
Amplicon (16S / 18S / ITS) platform yog tsim los nrog ntau xyoo ntawm kev paub nyob rau hauv microbial ntau haiv neeg qhov kev tsom xam, uas muaj cov qauv kev tshuaj ntsuam xyuas thiab kev txheeb xyuas tus kheej: kev tshuaj xyuas yooj yim npog cov ntsiab lus tseem ceeb ntawm kev tshawb fawb microbial tam sim no, cov ntsiab lus tsom xam yog nplua nuj thiab ua tiav, thiab kev soj ntsuam cov txiaj ntsig tau nthuav tawm hauv daim ntawv qhia txog qhov project;Cov ntsiab lus ntawm kev txheeb xyuas tus kheej muaj ntau yam.Cov qauv tuaj yeem xaiv tau thiab tsis tuaj yeem teeb tsa tau yooj yim raws li daim ntawv qhia kev txheeb xyuas thiab kev tshawb fawb lub hom phiaj, kom paub txog cov kev xav tau ntawm tus kheej.Windows operating system, yooj yim thiab ceev.
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Evolutionary noob caj noob ces
Evolutionary noob caj noob ces yog ib qho kev pab cuam sib luag tsim los muab kev txhais lus dav dav ntawm cov ntaub ntawv hloov pauv ntawm cov khoom siv raws li kev hloov pauv caj ces, suav nrog SNPs, InDels, SVs thiab CNVs.Nws muab tag nrho cov kev txheeb xyuas tseem ceeb uas yuav tsum tau piav qhia txog cov kev hloov pauv hloov pauv thiab cov yam ntxwv ntawm cov noob caj noob ces, xws li cov qauv pej xeem, kev sib txawv ntawm caj ces, kev sib raug zoo ntawm phylogeny, thiab lwm yam.
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Sib piv Genomics
Kev sib piv genomics txhais tau hais tias sib piv cov genome ua tiav thiab cov qauv ntawm ntau hom.Qhov kev qhuab qhia no yog tsom rau kev nthuav tawm hom kev hloov pauv, kev ua haujlwm ntawm cov noob caj noob ces, cov txheej txheem tswj cov txheej txheem ntawm qib genome los ntawm kev txheeb xyuas cov qauv kab ke thiab cov ntsiab lus uas khaws cia lossis sib txawv ntawm ntau hom.Kev sib piv genomics txoj kev tshawb fawb suav nrog kev txheeb xyuas hauv tsev neeg gene, evolutionary txoj kev loj hlob, tag nrho genome duplication, xaiv lub siab, thiab lwm yam.
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Evolutionary noob caj noob ces
Cov pej xeem thiab evolutionary genetic tsom xam platform yog tsim los ntawm lub hauv paus ntawm loj heev kev sau nyob rau hauv BMK R & D pab neeg rau xyoo.Nws yog cov cuab yeej siv tus phooj ywg tshwj xeeb tshaj yog rau cov kws tshawb fawb uas tsis loj hauv bioinformatics.Lub platform no tso cai rau cov kev hloov pauv ntawm cov noob caj noob ces cuam tshuam txog kev txheeb xyuas qhov tseem ceeb suav nrog kev tsim kho tsob ntoo phylogenetic, kev sib txuas tsis sib xws, kev ntsuam xyuas kev sib txawv ntawm caj ces, xaiv cheb tsom xam, kev txheeb xyuas kev txheeb ze, PCA, kev txheeb xyuas pej xeem cov qauv, thiab lwm yam.
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Hi-C raws li Genome Assembly
Hi-C yog ib txoj hauv kev tsim los ntes chromosome configuration los ntawm kev sib txuas kev soj ntsuam raws li kev sib cuam tshuam thiab kev sib tw siab.Qhov kev siv ntawm cov kev sib cuam tshuam no ntseeg tau tias muaj kev cuam tshuam tsis zoo nrog lub cev nyob deb ntawm chromosomes.Yog li, Hi-C cov ntaub ntawv tuaj yeem coj cov pawg, kev txiav txim thiab kev taw qhia ntawm cov kab ke sib dhos hauv cov txheej txheem genome thiab khi rau cov uas mus rau qee qhov chromosomes.Cov thev naus laus zis no txhawb nqa lub chromosome-theem genome los ua ke thaum tsis muaj cov pej xeem raws li daim ntawv qhia caj ces.Txhua lub genome xav tau Hi-C.
Platform: Illumina NovaSeq Platform / DNBSEQ
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Nroj / Tsiaj De Novo Genome Sequencing
De Novosequencing yog hais txog kev tsim kho ntawm ib hom genome tag nrho siv cov thev naus laus zis sib txuas, xws li PacBio, Nanopore, NGS, thiab lwm yam, thaum tsis muaj kev siv genome.Qhov kev txhim kho zoo kawg nkaus hauv kev nyeem ntawv ntev ntawm peb tiam thib peb sequencing technologies tau coj lub cib fim tshiab hauv kev sib sau ua ke ntawm cov genomes, xws li cov neeg uas muaj heterozygosity siab, siab piv ntawm cov cheeb tsam rov ua dua, polyploids, thiab lwm yam. Nrog rau kev nyeem ntev ntawm kaum ntawm kilobases qib, cov kev sib txuas lus no tau pab. daws cov ntsiab lus rov ua dua, thaj chaw uas muaj cov ntsiab lus GC txawv txav thiab lwm thaj chaw nyuaj heev.
Platform: PacBio Sequel II / Nanopore PromethION P48 / Illumina NovaSeq Platform
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Human Whole Exome Sequencing
Tag nrho exome sequencing (WES) yog suav hais tias yog ib qho txiaj ntsim sequencing zoo rau kev txheeb xyuas cov kab mob uas ua rau muaj kev hloov pauv.Txawm hais tias exons tsuas yog siv li ntawm 1.7% ntawm tag nrho genome, nws sawv cev rau qhov profile ntawm tag nrho cov protein ua haujlwm ncaj qha.Hauv tib neeg genome, nws tau raug tshaj tawm tias ntau dua 85% ntawm cov kab mob cuam tshuam txog kev hloov pauv tshwm sim hauv cheeb tsam protein coding.
BMKGENE muab cov kev pabcuam dav dav thiab hloov tau yooj yim tib neeg tag nrho cov kev pabcuam exome sequencing nrog txawv exon capturing tswv yim muaj kom tau raws li ntau yam kev tshawb fawb lub hom phiaj.
Platform: Illumina NovaSeq Platform
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Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)
High-throughput genotyping, tshwj xeeb tshaj yog nyob rau hauv loj-scale pej xeem, yog ib tug tseem ceeb kauj ruam nyob rau hauv cov kev tshawb fawb ntawm genetic koom haum, uas muab genetic hauv paus rau functional gene discovery, evolutionary tsom xam, thiab lwm yam ) yog qhia kom txo qis tus nqi sequencing ib tus qauv, thaum tswj kev ua haujlwm tsim nyog ntawm kev tshawb nrhiav caj ces.Qhov no feem ntau ua tiav los ntawm kev rho tawm qhov txwv tsis pub dhau ntawm qhov loj me, uas yog lub npe txo qis cov tsev qiv ntawv (RRL).Specific-locus amplified fragment sequencing (SLAF-Seq) yog ib lub tswv yim tsim kho tus kheej rau SNP genotyping nrog lossis tsis muaj kev siv genome.
Platform: Illumina NovaSeq Platform -
Illumina thiab BGI
Illumina sequencing thev naus laus zis, raws li Sequencing los ntawm Synthesis (SBS), yog lub ntiaj teb embraced NGS innovation, lub luag hauj lwm rau generating tshaj 90% ntawm lub ntiaj teb no sequencing cov ntaub ntawv.Lub hauv paus ntsiab lus ntawm SBS suav nrog cov duab fluorescently sau cov ntawv thim rov qab raws li txhua qhov dNTP tau ntxiv, thiab tom qab ntawd tau txiav kom tso cai rau kev koom ua ke ntawm lub hauv paus tom ntej.Nrog rau tag nrho plaub qhov thim rov qab-txhim kho dNTPs tam sim no nyob rau hauv txhua lub voj voog sib luag, kev sib tw ntuj txo qis kev sib koom ua ke.Qhov kev siv thev naus laus zis no txhawb nqa ob qho tib si nyeem ib leeg thiab ua ke-kawg cov tsev qiv ntawv, ua rau ntau yam kev siv genomic.Illumina sequencing's high-throughput peev xwm thiab precision qhov chaw nws yog lub hauv paus hauv kev tshawb fawb genomics, txhawb cov kws tshawb fawb los daws qhov tsis sib xws ntawm cov genomes nrog cov ntsiab lus tsis sib xws thiab kev ua haujlwm zoo.
DNBSEQ, tsim los ntawm BGI, yog lwm qhov tshiab NGS thev naus laus zis uas tau tswj hwm kom txo qis cov nqi sib txuas ntxiv thiab nce kev nce qib.Kev npaj ntawm DNBSEQ cov tsev qiv ntawv suav nrog DNA fragmentation, kev npaj ntawm ssDNA thiab dov lub voj voog kom tau txais cov DNA nanoballs (DNB).Cov no yog tom qab ntawd thauj mus rau qhov chaw ruaj khov thiab tom qab sib txuas los ntawm combinatorial Probe-Anchor Synthesis (cPAS).
Peb lub tsev qiv ntawv ua ntej ua tiav cov kev pabcuam pab txhawb cov neeg siv khoom hauv kev npaj lawv cov tsev qiv ntawv sib txuas los ntawm ntau qhov chaw (mRNA, tag nrho genome, amplicon, thiab lwm yam).Tom qab ntawd, cov tsev qiv ntawv no tuaj yeem raug xa mus rau peb cov chaw sib txuas ua ke rau kev tswj xyuas zoo thiab sib txuas hauv Illumina lossis BGI platforms.
