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Cov tsev qiv ntawv DNBSEQ uas tau ua tiav lawm
DNBSEQ, tsim los ntawm MGI, yog ib qho thev naus laus zis NGS tshiab uas tau tswj kom txo qis tus nqi sequencing thiab ua kom muaj txiaj ntsig ntau dua. Kev npaj cov tsev qiv ntawv DNBSEQ suav nrog DNA fragmentation, kev npaj ssDNA, thiab dov lub voj voog amplification kom tau txais DNA nanoballs (DNB). Cov no tom qab ntawd raug thauj mus rau ntawm qhov chaw khov kho thiab tom qab ntawd sequenced los ntawm combinatorial Probe-Anchor Synthesis (cPAS). DNBSEQ thev naus laus zis sib xyaw ua ke cov txiaj ntsig ntawm kev muaj qhov yuam kev amplification qis nrog kev siv cov qauv yuam kev ceev ceev nrog nanoballs, ua rau sequencing nrog throughput siab dua thiab raug.
Peb qhov kev pabcuam sequencing uas tau ua tiav lawm ua rau cov neeg siv khoom tuaj yeem npaj Illumina sequencing libraries los ntawm ntau qhov chaw sib txawv (mRNA, tag nrho genome, amplicon, 10x libraries, thiab lwm yam), uas tau hloov mus rau MGI libraries hauv peb lub chaw soj nstuam kom raug sequenced hauv DNBSEQ-T7, ua rau muaj cov ntaub ntawv ntau ntawm cov nqi qis dua.
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Cov tsev qiv ntawv Illumina uas tau ua tiav lawm
Cov thev naus laus zis Illumina sequencing, raws li Sequencing by Synthesis (SBS), yog ib qho kev tsim kho tshiab NGS thoob ntiaj teb, uas yog lub luag haujlwm rau kev tsim ntau dua 90% ntawm cov ntaub ntawv sequencing thoob ntiaj teb. Lub hauv paus ntsiab lus ntawm SBS suav nrog kev thaij duab fluorescently labeled reversible terminators thaum txhua dNTP raug ntxiv, thiab tom qab ntawd cleaved kom tso cai rau kev koom ua ke ntawm lub hauv paus tom ntej. Nrog rau tag nrho plaub reversible terminator-bound dNTPs uas muaj nyob rau hauv txhua lub voj voog sequencing, kev sib tw ntuj tsim txo qis kev sib xyaw ua ke. Cov thev naus laus zis ntau yam no txhawb nqa ob qho tib si nyeem ib leeg thiab khub-kawg tsev qiv ntawv, ua kom haum rau ntau yam kev siv genomic. Illumina sequencing lub peev xwm siab-throughput thiab qhov tseeb tso nws ua lub hauv paus tseem ceeb hauv kev tshawb fawb genomics, txhawb nqa cov kws tshawb fawb kom daws qhov nyuaj ntawm genomes nrog cov ntsiab lus tsis sib xws thiab kev ua haujlwm zoo.
Peb qhov kev pabcuam sequencing uas tau ua tiav lawm ua rau cov neeg siv khoom tuaj yeem npaj cov tsev qiv ntawv sequencing los ntawm ntau qhov chaw (mRNA, tag nrho cov genome, amplicon, 10x cov tsev qiv ntawv, thiab lwm yam). Tom qab ntawd, cov tsev qiv ntawv no tuaj yeem xa mus rau peb lub chaw sequencing rau kev tswj xyuas zoo thiab sequencing hauv Illumina platforms.
