Cov khoom

Prokaryotic RNA sequencing

Prokaryotic RNA sequencing siv cov tiam tom ntej sequencing (NGS) los qhia qhov muaj thiab ntau ntawm RNA ntawm ib lub sijhawm, los ntawm kev tshuaj xyuas qhov hloov pauv ntawm cov ntaub ntawv ntawm tes.Peb lub tuam txhab prokaryotic RNA sequencing, tshwj xeeb yog lub hom phiaj ntawm prokaryotes nrog kev siv genomes, muab koj cov ntaub ntawv sau tseg, kev txheeb xyuas cov qauv noob, thiab lwm yam.

Platform: Illumina NovaSeq Platform

Tiv tauj peb

Cov ntsiab lus kev pabcuam

Service Specifications

Platform: Illumina NovaSeq platform, PE150

Cov tsev qiv ntawv hom: 350bp ntxig cDNA lub tsev qiv ntawv (nyob ntawm qhov kev faib tawm siab tshaj)

Sequencing lub tswv yim: Paired-End 150 bp

Pom zoo cov ntaub ntawv tus nqi: 2 Gb raw cov ntaub ntawv / qauv

Bioinformatics

(1) Sequencing data quality control: Nucleotide distribution, Base zoo distribution, rRNA ratio assessment;

(2) Kev txheeb xyuas qhov sib npaug: Kev txheeb xyuas qhov tshwm sim, Saturation ntawm sequencing, Kev pab them nqi sequencing;

(3) Reference genome annotation (NR, Swiss-Prot, Pfam, COG, GO, KEGG);

(4) Kev tsom xam kev nthuav qhia: Kev nthuav qhia kev nthuav qhia (Nrog ob lossis ntau qhov qauv), Kev ntsuam xyuas kev qhia ntawm cov qauv (Venn tsom xam, Kev txheeb xyuas kev sib txheeb, kev txheeb xyuas PCA);

(5) Kev txheeb xyuas qhov sib txawv (Nrog ob lossis ntau qhov qauv);

(6) Gene set analysis: Venn analysis, Cluster analysis, Function annotation analysis (COG, GO, KEGG), Function enrichment analysis (GO, KEGG), Function enrichment chordal graph, Ipath analysis;

(7) sRNA tsom xam: sRNA twv ua ntej, sRNA annotation (Blast, sRNAMap, sRNATarBase, SIPHT, Rfam), sRNA theem nrab qauv kwv yees, sRNA lub hom phiaj gene kwv yees;

(8) Transcript structure analysis: Operaon analysis, Transcription start and end sites twv twv, UTR annotation thiab tsom xam, Promoter sequence prediction, SNP/InDel analysis (SNP/InDel annotation, SNP/InDel regional distribution, SNP statistics).