Kev kawm txog Proteomics
Cov yam ntxwv
●Cov Proteomics Zoo: Siv LC-MS/MS los txheeb xyuas cov protein sib xyaw hauv cov qauv sib xyaw (SDS-PAGE gel strips, IP, Co-IP, Pull-down). Cov Zoo: Tsis muaj kev txwv tus lej qauv, nrhiav tau sai, ua cov qauv yooj yim, muaj peev xwm ua haujlwm tau zoo, thiab muaj peev xwm nrhiav tau cov protein tsawg.
● Kev Siv Cov Proteomics Uas Tsis Muaj Daim Ntawv Cim: Cov thev naus laus zis uas tsis muaj daim ntawv cim nrog kev kuaj pom cov qauv ib tus zuj zus. Ntsuas cov protein los ntawm kev sib piv cov teeb liab peptide hauv cov ntaub ntawv mass spectrometry. Cov Zoo: Kev ua haujlwm yooj yim, kev xa khoom sai (tsis muaj kev txwv tus lej qauv), thiab kev siv tau dav (haum rau "muaj/tsis muaj" kev sib piv cov protein sib txawv ntawm ntau hom).
● DIA Quantitative Proteomics: Siv hom kev nrhiav cov ntaub ntawv (DIA), luam theej tag nrho cov ions hauv cov qhov rai segmented kom ntes tau cov ntaub ntawv ion tag nrho. Cov Zoo: Rov ua dua tau zoo dua, kev them cov protein ntau dua, thiab kev ntsuas ntau dua li hom DDA (TMT/Label Free), zoo tagnrho rau kev tshawb fawb loj.
● 4D-Label Free Quantitative Proteomics/4D-DIA Quantitative Proteomics: Raws li Bruker timsTOF mass spectrometer, ntxiv ion mobility (collision cross-section) rau kev sib cais 3D ib txwm muaj. Cov txiaj ntsig: Kev siv ion thiab kev raug zoo dua, kev txhim kho kom zoo dua hauv qhov tob ntawm kev npog, kev nkag siab thiab kev xa tawm; qhov tob ntawm kev txheeb xyuas ntau dua li txoj kev 3D ib txwm muaj.
● Astral Label Free Quantitative Proteomics/Astral DIA Quantitative Proteomics: Raws li OrbitrapTM AstralTM high-resolution mass spectrometer. Cov Zoo: Kev ua haujlwm ntau dua (100+ qauv/hnub nrog 8-feeb gradient), kev npog tob dua (8,000+ cov protein pom hauv Hela hlwb hauv 8 feeb), thiab kev rhiab heev dua (tsis tas yuav kuaj ntau).
● TMT Quantitative Proteomics: Siv 18 daim ntawv cim isotope los sau cov peptides rau kev nrhiav tau 18 tus qauv tib lub sijhawm. Cov Zoo: Kev ruaj khov siab (kev cuam tshuam tsawg kawg nkaus ntawm cov cuab yeej ruaj khov, qhov yuam kev me me ntawm lub kaw lus) thiab kev nkag siab siab (kev faib ua feem txo qhov nyuaj ntawm cov qauv, txhim kho kev nrhiav tau cov protein tsawg).
● PRM Targeted Proteomics: Cov thev naus laus zis siab rau kev nrhiav cov protein/peptides uas xav tau. Cov Zoo: Ua kom muaj kev ntsuas thiab kev txheeb xyuas cov txiaj ntsig ntawm cov protein; tsis muaj kev txwv rau cov tshuaj tiv thaiv kab mob, siv tau dav dua li Western Blot thiab ELISA.
Cov txiaj ntsig
● Cov Khoom Siv Siab Tshaj Plaws: Nruab nrog ob qho tib si cov platform proteomics ib txwm muaj thiab cov spectrometers siab heev (piv txwv li, 4D, Astral).
● Kev Tshawb Pom Ruaj Kho: Cov txheej txheem tswj xyuas zoo kom ntseeg tau tias cov txheej txheem kuaj mob sib xws thiab ruaj khov.
● Cov Txiaj Ntsig Txhim Khu Kev: Kev tshuaj xyuas zoo thiab ntau yam tib lub sijhawm muab cov lus qhia sib piv, qhov hnyav ntawm cov molecular, qhov muaj ntau thiab lwm cov ntaub ntawv tseem ceeb rau txhua pawg.
● Kev Siv Tshuab Siab: Cov txheej txheem LC-MS uas siv tshuab ua rau muaj kev tshuaj xyuas sai thiab kev sib cais zoo heev.
Cov Lus Qhia Txog Kev Pabcuam
| Kev Sib Piv ntawm Cov Txheej Txheem Proteomics Tsis Yog Lub Hom Phiaj | ||||
| Tsis muaj daim ntawv lo | DIA | TMT | ||
| Kev sau ntawv lo | NO | NO | YOG | |
| Hom Kev Ntsuas Cov Ntaub Ntawv | DDA | DDA | DIA | DDA |
| Kev ua dua tshiab | Qis dua | Siab | Siab | |
| Kev rhiab heev | Qis dua | Siab | Siab | |
| Kev sib txuas ntau yam | NO | YOG | NO | YOG |
| Daim Ntawv Thov | Kev sib piv ntawm cov protein muaj / tsis muaj | Cov qauv loj | Kev tshuaj xyuas ntawm cov pawg sib txawv ntawm cov qauv | Kev sib piv ntawm cov protein sib txawv nyob rau hauv tib hom thiab cov ntaub so ntswg |
| Kev raug | ★ | ★★(4D/Astral DlA ★★★) | ★★ | |
| Kev suav ntawm kev kuaj pom | ★ | ★★(4D/Astral D|A★★★) | ★★ | |
Cov Qauv Yuav Tsum Tau Ua
Koj puas xav paub seb koj cov qauv puas ua tau raws li peb cov qauv? Nyem qhov no kom tau txais peb cov qauvcov qauv xav tau tshiab tshaj plaws.
Kev Ua Haujlwm Pabcuam
Cov Qauv Sau
Kev rho tawm cov protein
Kev zom zaub mov enzymatic
Kev sib cais
Kev Tau Txais Cov Ntaub Ntawv
Kev Tshawb Fawb Txog Cov Ntaub Ntawv
Kev Xa Cov Ntaub Ntawv
Kev Tshawb Fawb Txog Proteomics Zoo
1. Cov kua protein/gel: cov lus qhia txog cov txiaj ntsig zoo
2. Cov Txiaj Ntsig Tshawb Nrhiav Hauv Database
2.1 Daim ntawv teev cov peptide segments sib phim hauv kev tshawb nrhiav database
2.2 Daim ntawv teev cov protein sib phim hauv kev tshawb nrhiav hauv database
2.3 Daim ntawv teev cov kev hloov kho uas phim hauv kev tshawb nrhiav hauv database (phosphorylation, ubiquitination, thiab lwm yam)
3. Cov ntaub ntawv raw
Kev ntsuas Proteomics(Tsis Muaj Daim Ntawv/DIA/TMT)
1. Kev Ua Ntej Cov Ntaub Ntawv
1.1 Kev Tshawb Nrhiav Cov Ntaub Ntawv Protein
2. Kev Tshawb Fawb Txog Cov Protein
2.1 Kev Tshawb Fawb Txog Cov Cheebtsam Tseem Ceeb (PCA)
2.2 Kev Sib Txawv Tus Qauv (RSD)
2.3 K-txhais tau tias Kev Faib Tawm Qauv
2.4 Kev Ntsuam Xyuas Qhov Rov Ua Dua Tau
2.5 Daim Ntawv Qhia Kub ntawm Cov Protein Qhia Tawm
3. Cov Lus Qhia Txog Kev Ua Haujlwm
3.1 GO Cov Lus Qhia Txog Kev Ua Haujlwm
3.2 KEGG Cov Lus Qhia Txog Kev Ua Haujlwm
3.3 COG Cov Lus Qhia Txog Kev Ua Haujlwm
3.4 GOslim Cov Lus Qhia Txog Kev Ua Haujlwm
3.5 Pfam Cov Qauv Protein Domain Annotation
4. Kev Tshawb Fawb Txog Qhov Txawv ntawm Cov Protein (cov qauv tshuaj lom neeg ≥ 3)
4.1 Cov txiaj ntsig ntawm kev tshuaj xyuas qhov sib txawv ntawm cov protein
4.2 Kev Faib Tawm ntawm Cov Kev Hloov Pauv Protein Sib Txawv (FC)
4.3 Kev Tshawb Fawb Txog Qhov Muaj Protein Sib Txawv
4.4 Daim Duab Qhia Txog Qhov Sib Txawv ntawm Cov Protein Volcano
4.5 Daim Ntawv Qhia Kub ntawm Cov Protein Sib Txawv
4.6 Cov Lus Cim Ua Haujlwm ntawm Cov Protein GO thiab Kev Tshawb Fawb Txog Kev Nce Qib
4.7 Cov Lus Cim Ua Haujlwm ntawm Cov Protein Sib Txawv KEGG thiab Kev Tshawb Fawb Txog Kev Txhim Kho
4.8 Cov Lus Qhia Txog Kev Ua Haujlwm ntawm Cov Protein COG Sib Txawv
4.9 Cov Lus Cim Qhia Txog Cov Protein Sib Txawv GOslim thiab Kev Tshawb Fawb Txog Kev Txhim Kho
4.10 Cov Lus Cim Qhia Txog Cov Qauv Protein Sib Txawv thiab Kev Tshawb Fawb Txog Kev Nce Qib
5. Kev Tshawb Fawb Txog Kev Sib Txuas Lus ntawm Cov Protein Network
6. Cov Lus Qhia Txog Txoj Kev Ua Protein Reactome
7. Kev Kwv Yees Peptide Teeb Liab
8. Kev Tshawb Fawb Txog Cov Protein Hauv Cov Cell
Kev ntsuas Proteomics(PRM)
1. Cov Txiaj Ntsig ntawm Kev Ntsuas Cov Protein PRM
1.1 Kev Txheeb Xyuas Txog Cov Txiaj Ntsig ntawm Kev Ntsuas
1.2 Kev Faib Tawm ntawm Cov Cheeb Tsam Fragment Ion Peak rau Peptide Segments
Kev ntsuas Proteomics
1.Kev Tshawb Fawb Txog Cov Protein
↑Kev Tshawb Fawb Txog Cheebtsam Tseem Ceeb (PCA)↑Kev Sib Txawv Tus Qauv (RSD)
↑K-txhais tau tias Kev Faib Tawm Qauv ↑Kev Sib Raug ZooAkev tshuaj xyuas ntawmProtineEkev qhia tawmLcov evels
↑ Daim Ntawv Qhia Kub ntawm Cov Protein Qhia
2. Cov Lus Qhia Txog Kev Ua Haujlwm
↑GO Cov Lus Qhia Txog Kev Ua Haujlwm↑KEGG Cov Lus Qhia Txog Kev Ua Haujlwm
↑ COG Cov Lus Cim Ua Haujlwm ↑ GOslim Cov Lus Cim Ua Haujlwm
↑ Cov Lus Qhia Txog Lub Npe ntawm Pfam Protein Structure
3.Kev Tshawb Fawb Txog Kev Sib Txuas Lus ntawm Protein Network
↑ Kev Tshawb Fawb Txog Kev Sib Cuam Tshuam ntawm Cov Protein Network
4.Kev Kwv Yees Peptide Teeb Liab
↑ Kev Kwv Yees Peptide Teeb Liab
5. Kev Tshawb Fawb Txog Cov Proteins Hauv Cov Cell
↑Kev Tshawb Fawb Txog Cov Protein Hauv Cov Cell
| 2025 | Cov Vesicles Apoptotic Uas Tau Los Ntawm Mesenchymal Stem Cells Ua Rau Cov Lus Teb Hypersensitivity Zoo Dua ntawm Kev Ua Rau CD8+ T Cells Apoptosis Nrog Calcium Overload Thiab Mitochondrial Dysfunction | Kev Tshawb Fawb Qib Siab |
| 2024 | Kev sib xyaw ua ke ntawm ntau yam tshuaj ua rau pom tias muaj txiaj ntsig zoo ntawm kev tiv thaiv qog nqaij hlav | Kev Kho Mob Oncology Txhais Lus |
| 2024 | Kev Tshuaj Xyuas Multi-Omics Qhia Txog Cov Yam Ntxim Saib Sib Txawv ntawm Cov Kev Ua Haujlwm Metabolism Txhawb Nqa Qhov Loj thiab Xim ntawm Cov Txiv Hmab Txiv Ntoo thiab Taub Dag Loj | Int. J. Mol. Sci. |
| 2024 | Kev Tshawb Fawb Txog Kev Sau Ntawv thiab Kev Tshawb Fawb Txog Metabolome Muab Kev Nkag Siab Tshiab rau Kev Tsis Siv Cov Nqaij Hlav Atrophy hauv Nqaij Qaib: Lub Luag Haujlwm ntawm Cov Nqaij Hnyuv Ceev Ceev | Int. J. Mol. Sci. |
| 2023 | Kev tshuaj xyuas ntau yam qhia txog kev cuam tshuam ntawm tetracycline rau kev loj hlob ntawm cov hauv paus ryegrass. | Phau ntawv xov xwm txog cov khoom phom sij |
