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Cov khoom

Ib leeg- nucleus RNA Sequencing

Kev nce qib hauv tib lub xov tooj ntawm tes tuav thiab ib tus neeg siv tsev qiv ntawv tsim cov txheej txheem sib txuas nrog kev sib txuas ua haujlwm siab tso cai rau cov kev tshawb fawb gene ntawm cov xov tooj ntawm tes.Nws ua kom muaj kev sib sib zog nqus thiab ua tiav kev tshuaj xyuas ntawm cov cell complex, nyob rau hauv uas nws feem ntau zam qhov npog ntawm lawv cov heterogeneity los ntawm kev noj nruab nrab ntawm tag nrho cov hlwb.

Txawm li cas los xij, qee lub hlwb tsis tsim nyog los ua rau hauv ib leeg-cell ncua kev kawm, yog li lwm cov qauv kev npaj - xav tau kev rho tawm nucleus los ntawm cov ntaub so ntswg, uas yog, nucleus raug rho tawm ncaj qha los ntawm cov ntaub so ntswg lossis cell thiab npaj rau hauv ib qho kev ncua rau ib leeg- cell sequencing.

BMK muab 10 × Genomics ChromiumTM raws li ib leeg-cell RNA sequencing kev pabcuam.Cov kev pabcuam no tau siv dav hauv kev tshawb fawb txog kev tshawb fawb txog kab mob, xws li kev tiv thaiv kab mob sib txawv, qog heterogeneity, kev loj hlob ntawm cov ntaub so ntswg, thiab lwm yam.

Spatial transcriptome nti: 10 × Genomics

Platform: Illumina NovaSeq Platform


Cov ntsiab lus kev pabcuam

Bioinformatics

Cov txiaj ntsig Demo

Technical txoj cai

Kev sib cais ntawm nuclei yog ua tiav los ntawm 10 × Genomics ChromiumTM, uas muaj yim-channel microfluidics system nrog ob txoj kev hla.Hauv qhov system no, gel hlaws nrog barcodes thiab primer, enzymes thiab ib lub nucleus yog encapsulated nyob rau hauv nanoliter-loj roj poob, generating Gel Bead-in-Emulsion (GEM).Thaum GEM raug tsim, cell lysis thiab tso tawm barcodes tau ua nyob rau hauv txhua GEM.mRNA yog thim rov qab rau hauv cDNA molecules nrog 10 × barcodes thiab UMI, uas yog ntxiv rau cov qauv sib txuas hauv tsev qiv ntawv.

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Cov ntaub so ntswg tsis haum rau kev npaj ib leeg ntawm tes

Cell / daim ntaub

Yog vim li cas

Unfresh khov cov ntaub so ntswg

Tsis tuaj yeem tau txais cov koom haum tshiab lossis kev txuag ntev

Muscle cell, Megakaryocyte, Fat…

Cell txoj kab uas hla yog loj dhau mus rau hauv lub ntsuas

Lub siab…

Yooj yim heev rau tawg, tsis tuaj yeem paub qhov txawv ntawm ib leeg

Neuron cell, Lub hlwb…

Ntau rhiab heev, yooj yim rau kev ntxhov siab, yuav hloov cov txiaj ntsig sib luag

Pancreas, thyroid…

nplua nuj nyob rau hauv endogenous enzymes, cuam tshuam rau zus tau tej cov cell suspension

Single-nucleus vs Single-cell

Ib leeg-nucleus

Ib leeg xwb

Unlimited cell diam

Cell txoj kab uas hla: 10-40 μm

Cov khoom tuaj yeem yog cov ntaub so ntswg khov

Cov khoom yuav tsum yog cov ntaub so ntswg tshiab

Tsawg kev ntxhov siab ntawm cov cell khov

Kev kho enzyme tuaj yeem ua rau muaj kev ntxhov siab ntawm tes

Tsis muaj cov qe ntshav liab yuav tsum tau muab tshem tawm

Cov qe ntshav liab yuav tsum tau muab tshem tawm

Nuclear qhia txog bioinformation

Tag nrho cov cell qhia txog bioinformation

Service Specifications

Lub tsev qiv ntawv

Sequencing tswv yim

Cov ntaub ntawv ntim

Cov Qauv Xav Tau

Cov ntaub so ntswg

10 × Genomics single-nuclei tsev qiv ntawv

10x Genomics -Illumina PE150

100,000 nyeem / cell approx.100-200 Gb

Cell naj npawb:> 2 × 105

Cell conc.ntawm 700-1,200 cell / μL

≥ 200 mg

Yog xav paub ntxiv txog cov qauv qhia kev npaj thiab kev pabcuam kev ua haujlwm, thov koj tham nrog a

Kev Pabcuam Ua Haujlwm Flow

Qauv QC

Kev sim tsim

coj mus kuaj

Qauv xa tuaj

nuclear. ncua

Kev cais tawm ntawm Nuclei

Kev npaj tsev qiv ntawv

Kev tsim kho tsev qiv ntawv

Sequencing

Sequencing

Cov ntaub ntawv tsom xam

Cov ntaub ntawv tsom xam

Kev pab cuam tom qab muag

Kev pab cuam tom qab muag


  • Yav dhau los:
  • Tom ntej:

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    1.Spot clustering

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    2.Marker qhia abundance clustering heatmap

    wps_doc_113.Maker noob faib nyob rau hauv ntau pawg

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    4.Cell trajectory tsom xam/pseudotime

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    tau ib nqe lus

    Sau koj cov lus ntawm no thiab xa tuaj rau peb

    Xa koj cov lus rau peb: