Kev Txhawb Nqa Lub Sijhawm Txwv: Kev daws teeb meem sib dhos T2T Genome All-in-One
Nrog rau kev txhim kho sai ntawm cov thev naus laus zis genomic sequencing thiab cov txheej txheem sib dhos, Telomere-rau-Telomere (T2T) genome sib dhos tau dhau los ua tus qauv kub rau kev tshawb fawb genome zoo, daws qhov teeb meem sib txawv ntev hauv kev sib dhos genome ib txwm muaj (Cov cheeb tsam uas muaj cov rov ua dua siab thiab cov ntsiab lus GC siab, xws li telomeres thiab centromeres, xav tau cov ntaub ntawv sequencing ntev heev kom nthuav dav lawv kom ua tiav). Txhawm rau txhawb kev nrov npe ntawm T2T thev naus laus zis hauv kev tshawb fawb kev kawm, tau txais cov kab ke genome tiav ntau dua, thiab ua kom pom cov noob caj noob ces thiab cov qauv sib txawv uas tsis tau lees paub yav dhau los, peb tso tawm qhov kev txhawb nqa lub sijhawm txwv no, muab kev daws teeb meem sib dhos genome T2T uas pheej yig rau koj hom tsiaj, txhawb koj txoj kev tshawb fawb nrog kev siv tiav thiab raug.
Cov Lus Qhia Txog Pob Khoom Pabcuam
●Kev txheeb xyuas kab ke
● Cov ntawv nyeem luv luv ntawm Illumina rau Kev Tshawb Fawb: 50X
● PacBio HiFi nyeem rau kev sib dhos qauv: 50X
● Hi-C rau scaffolding: 100X
● Nanopore nyeem ntev heev rau kev sau qhov sib txawv: 2 lub hlwb
● Illumina mRNA-seq rau genome annotation: 12 Gb / 1 pool
●Kev Tshawb Fawb Txog Qib Siab
● Kev soj ntsuam rau kev ntsuam xyuas cov yam ntxwv ntawm genome
● Kev sib dhos rau contig theem genome
● Scaffolding rau chromosome theem genome
● Kev sau qhov sib txawv thiab kev txheeb xyuas telomere rau T2T genome
● Kev ntsuam xyuas qhov zoo ntawm T2T genome
● Cov lus piav qhia txog genome rau kev kwv yees cov ntsiab lus ntawm gene thiab genomics
● Daim ntawv qhia txog kev tshuaj xyuas tiav nrog kev txhais lus tshaj lij
Cov txiaj ntsig ntawm kev sim
Yuav kom nkag siab zoo dua txog qhov zoo ntawm peb cov kev pabcuam sib dhos genome T2T, peb muab cov txiaj ntsig ntawm qhov project ib txwm muaj:
•Lub genome T2T kawg muaj 19 chromosomes nrog tag nrho ntev ntawm 491.39Mb, thiab contig N50 ntawm 26.04Mb. Tag nrho ntawm 38 telomeres thiab 19 centromeres tau txheeb xyuas. 19 chromosomes yog cov chromosomes telomere-rau-telomere uas tsis muaj qhov sib txawv.
•Cov txiaj ntsig ntawm kev ntsuam xyuas kev sib dhos qhia tau tias muaj kev ua tiav thiab raug ntawm cov genome sib dhos: Kev ntsuam xyuas BUSCO siv cov ntaub ntawv embryophyta ntawm OrthoDB 10 qhia tau tias muaj kev ua tiav ntawm genome ntawm 97.09%; Tus nqi genome QV yog 52.36. Tus nqi genome LAI yog 18.93.
Kev siv tau
•Cov teb tshawb fawb siv tauKev tshawb fawb txog genomic yooj yim, kev txhim kho noob caj noob ces ntawm cov qoob loo, kev yug tsiaj, txhua qhov chaw cog qoob loo thiab tsiaj txhu uas xav tau kev sib sau ua ke ntawm genome zoo.
•Cov hom tsiaj siv tau: Qhov loj ntawm genome ≤ 1; Cov tsiaj txhu, cov qoob loo ib txwm muaj, cov txiv hmab txiv ntoo thiab cov txiv tsawb uas pom ntau, cov ntoo hav zoov, cov ntses dej qab zib, thiab lwm yam. Yog xav paub ntxiv txog lwm hom tsiaj, thov mus saib.
•Kev txhawb nqa siv tau: Cov ntawv cog lus tau kos npe ua ntej Lub Rau Hli 30.
•Cov lus qhia ntxivRau cov qauv tshwj xeeb (piv txwv li, cov uas muaj cov kab rov ua dua ntau zaus lossis cov DNA tsis zoo) lossis cov kev xav tau kev tshuaj xyuas tshwj xeeb, peb pab neeg ua haujlwm yuav muab kev daws teeb meem rau koj, nrog rau tus nqi hloov kho raws li qhov xav tau tiag tiag ntawm qhov project.
Pib Tam Sim No
Siv qhov kev tshaj tawm luv luv no thiab txaus siab rau qhov kev pabcuam sib dhos genome T2T zoo tshaj plaws ntawm tus nqi tshwj xeeb! Tiv tauj peb pab neeg tshaj lij kom tau txais cov ntaub ntawv ntxiv, xa cov ntaub ntawv piv txwv, thiab tau txais tus nqi tshwj xeeb.
