Kev txheeb xyuas tag nrho cov noob caj noob ces bisulfite (WGBS)
Cov txheej txheem no, raws li kev kho mob bisulfite ntawm DNA los ua kom muaj kev hloov pauv ntawm cov cytosines unmethylated mus rau uracil (C rau U), thaum tawm hauv cov cytosines methylated tsis hloov pauv, sawv cev rau txoj kev ntsuas kub rau kev tshawb nrhiav tob ntawm DNA methylation, tshwj xeeb tshaj yog qhov chaw thib tsib hauv cytosine (5-mC), tus tswj hwm tseem ceeb ntawm kev qhia tawm gene thiab kev ua haujlwm ntawm tes.
Cov txheej txheem no muab kev daws teeb meem ib leeg, uas tso cai rau cov kws tshawb fawb tshawb nrhiav cov methylome thiab nrhiav pom cov qauv methylation tsis zoo hauv cov qauv kuaj. Los ntawm kev siv WGBS, cov kws tshawb fawb tuaj yeem tau txais kev nkag siab tsis zoo txog cov toj roob hauv pes methylation thoob plaws genome, muab kev nkag siab zoo txog cov txheej txheem epigenetic uas yog lub hauv paus ntawm ntau yam txheej txheem biological thiab kab mob.
Cov yam ntxwv ntawm kev pabcuam
● Kev txheeb xyuas kab ke ntawm Illumina NovaSeq.
● Yuav tsum muaj ib qho genome siv ua piv txwv.
● Ntxiv Lambda DNA los saib xyuas kev hloov pauv bisulfite zoo.
Cov Kev Pabcuam Zoo
●Tus Qauv Kub rau Kev Tshawb Fawb Txog DNA Methylation: Cov thev naus laus zis no muaj kev raug siab thiab kev ua tau zoo.
●Kev Pab Dav Dav thiab Kev daws teeb meem ib leeg:Qhov kev tshawb pom ntawm cov chaw methylation yog nyob rau ntawm theem thoob plaws genome.
●Platform tiav:Peb muab kev pabcuam ib zaug xwb los ntawm kev ua cov qauv, kev tsim tsev qiv ntawv, kev txheeb xyuas cov ntaub ntawv mus rau kev tshuaj xyuas bioinformatics.
●Kev Paub Ntau YamPeb muaj ntau yam haujlwm ua tiav thoob plaws ntau hom tsiaj sib txawv. BMKGENE coj ntau tshaj kaum xyoo ntawm kev paub dhau los, pab pawg tshuaj xyuas kev txawj ntse, cov ntsiab lus tiav, thiab kev txhawb nqa tom qab muag zoo heev.
●Muaj peev xwm koom nrog Transcriptomics AnalysisTso cai rau kev tshuaj xyuas kev sib xyaw ua ke ntawm WGBS nrog lwm cov ntaub ntawv omics xws li RNA-seq.
Cov Qauv Qhia Txog
| Tsev qiv ntawv | Txoj Kev Npaj Txog Sequencing | Cov ntaub ntawv tso tawm pom zoo | Kev tswj xyuas zoo |
| Kho Bisulfite | Illumina PE150 | 30x qhov tob | Q30 ≥ 85% Kev hloov pauv ntawm Bisulfite > 99% |
Cov Qauv Yuav Tsum Tau Ua
| Kev Ntsuas (ng/µL) | Tag nrho cov nqi (µg) | Cov kev cai ntxiv | |
| DNA genomic | ≥ 5 | ≥ 400 ng | Kev puas tsuaj lossis kev ua qias tuaj tsawg |
Kev Ua Haujlwm Pabcuam
Kev xa khoom qauv
Kev rho tawm DNA
Kev tsim kho tsev qiv ntawv
Kev txheeb xyuas kab ke
Kev tshuaj xyuas cov ntaub ntawv
Kev xa cov ntaub ntawv
Cov kev pabcuam suav nrog cov kev tshuaj xyuas hauv qab no:
● Kev tswj xyuas qhov zoo ntawm cov ntaub ntawv raw;
● Kev kos duab rau cov genome siv;
● Kev nrhiav pom 5mC methylated bases;
● Kev tshuaj xyuas ntawm kev faib tawm methylation thiab kev piav qhia;
● Kev Tshawb Fawb Txog Cov Cheeb Tsam Methylated Sib Txawv (DMRs);
● Kev piav qhia txog kev ua haujlwm ntawm cov noob caj noob ces uas cuam tshuam nrog DMRs.
Kev kuaj pom methylation 5mC: hom methylated sites
Daim ntawv qhia methylation. 5mC methylation genome-thoob plaws kev faib tawm
Cov lus piav qhia ntawm cov cheeb tsam methylated heev
Cov Cheeb Tsam Methylated Sib Txawv: cov noob caj noob ces cuam tshuam
Cov Cheeb Tsam Methylated Sib Txawv: kev piav qhia txog cov noob caj noob ces cuam tshuam (Gene Ontology)
Tshawb nrhiav kev nce qib ntawm kev tshawb fawb uas tau pab txhawb los ntawm BMKGene cov kev pabcuam sequencing genome bisulfite tag nrho los ntawm kev sau cov ntawv tshaj tawm.
Fan, Y. et al. (2020) 'Kev tshuaj xyuas cov qauv DNA methylation thaum lub sijhawm txhim kho cov leeg nqaij ntawm cov yaj siv cov txheej txheem bisulfite tag nrho',BMC Genomics, 21(1), pp. 1–15. doi: 10.1186/S12864-020-6751-5.
Zhao, X. et al. (2022) 'Kev cuam tshuam tshiab ntawm deoxyribonucleic acid methylation hauv cov neeg ua haujlwm raug rau vinyl chloride',Kev Tshawb Fawb Txog Tshuaj Lom thiab Kev Noj Qab Haus Huv Hauv Kev Lag Luam, 38(7), pp. 377–388. Ib: 10.1177/07482337221098600
Zuo, J. et al. (2020) 'Kev sib raug zoo ntawm genome methylation, theem ntawm non-coding RNAs, mRNAs thiab metabolites hauv cov txiv lws suav siav',Phau Ntawv Xov Xwm Txog Cov Nroj Tsuag, 103(3), pp. 980–994. doi: 10.1111/TPJ.14778.
