Tag nrho genome bisulfite sequencing
Kev Pabcuam Zoo
● Ua kom tiav platform: muab ib qho kev pabcuam zoo tshaj plaws los ntawm kev ua qauv, kev tsim tsev qiv ntawv, sib txuas rau bioinformatics tsom xam.
● Cov neeg raug siab: Mature methylation conversion processing thev naus laus zis tuaj yeem txheeb xyuas qhov xwm txheej methylation ntawm ib qho C hauv paus.
● Kev pab dav dav: nrhiav pom cov chaw methylation ntawm qib genome-wide.
● Zoo rov ua tau zoo: kev ua haujlwm ruaj khov, haum rau kev sib piv ntawm ntau cov qauv.
Qauv Specifications
|
Platform
|
Nyeem Length
|
Sequence Strategy
|
| Illumina | PEB 150 | 30X, peb cov khoom siv roj ntsha, nrog cov khoom siv zoo genome |
Cov Qauv Xav Tau
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Hom qauv
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Tus nqi (μg)
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Conc.(nq/μl)
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Ntim (μl)
|
Purity
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| gDNA ua | ≥ 2 | ≥ 20
| ≥ 20
| Tsawg degradation thiab paug
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Kev Pabcuam Ua Haujlwm Flow
Qauv xa tuaj
DNA rho tawm
Kev tsim kho tsev qiv ntawv
Sequencing
Cov ntaub ntawv tsom xam
Kev xa cov ntaub ntawv
Bioinformatics
1.Genome-wide DNA methylation faib
2.Cov lus piav qhia ntawm thaj tsam CGI siab heev
