Tag nrho cov ntawv sau ua ke - Illumina
Nta
● Lub tsev qiv ntawv dual los ua kom tiav cov ntawv sau tseg: rRNA depletion tom qab los ntawm PE150 lub tsev qiv ntawv npaj thiab xaiv qhov loj raws li SE50 npaj tsev qiv ntawv
● Ua tiav bioinformatics tsom xam ntawm mRNA, lncRNA, circRNA thiab miRNA nyob rau hauv cais bioinformatics qhia
● Kev tshawb xyuas sib koom ua ke ntawm txhua qhov RNA qhia hauv kev qhia ua ke, suav nrog ceRNA kev txheeb xyuas cov tes hauj lwm.
Kev Pabcuam Zoo
●In-depth tsom xam ntawm kev tswj xyuas network: ceRNA network tsom xam yog qhib los ntawm kev sib koom ua ke ntawm mRNA, lncRNA, circRNA thiab miRNA thiab los ntawm kev ua haujlwm ntawm bioinformatic tag nrho.
●Kev piav qhia dav: peb siv ntau lub databases los ua haujlwm piav qhia qhov txawv ntawm Cov Genes (DEGs) thiab ua qhov kev txheeb xyuas qhov zoo sib xws, muab kev nkag siab ntawm cov txheej txheem cellular thiab molecular hauv qab cov lus teb.
●Kev txawj ntse: nrog cov ntaub ntawv teev tseg ntawm kev ua tiav kaw dhau 2000 tag nrho cov ntaub ntawv sau tseg hauv ntau qhov kev tshawb fawb sau npe, peb pab neeg coj ntau yam kev paub rau txhua qhov haujlwm.
●Rigorous Quality Control: peb siv cov ntsiab lus tseem ceeb hauv txhua theem, los ntawm cov qauv thiab kev npaj tsev qiv ntawv mus rau kev sib txuas lus thiab bioinformatics.Qhov kev soj ntsuam nruj me ntsis ua kom cov khoom xa tuaj raws li cov txiaj ntsig zoo.
● Cov lus piav qhia dav dav: peb siv ntau lub databases los ua haujlwm piav qhia qhov txawv ntawm Cov Genes (DEGs) thiab ua qhov kev txheeb xyuas qhov zoo sib xws, muab kev nkag siab ntawm cov txheej txheem cellular thiab molecular hauv qab cov lus teb.
●Kev them nyiaj yug tom qab muag: Peb qhov kev cog lus txuas ntxiv dhau qhov kev ua tiav nrog rau 3 lub hlis tom qab kev muag khoom.Nyob rau lub sijhawm no, peb muab cov phiaj xwm rov qab, kev pab daws teeb meem, thiab Q&A ntu los daws cov lus nug ntsig txog cov txiaj ntsig
Sample Requirements thiab Delivery
| Lub tsev qiv ntawv | Sequencing tswv yim | Cov ntaub ntawv pom zoo | Kev Tswj Xyuas Zoo |
| rRNA depleted | Illumina PE 150 | 16gb ib | Q30≥85% |
| Xaiv qhov loj me | Illumina SE 50 | 10-20M nyeem |
Cov Qauv Xav Tau:
Nucleotides:
| Conc.(ng/μl) | Tus nqi (μg) | Purity | Kev ncaj ncees |
| ≥100 | ≥ 1 | OD 260/280 = 1.7-2.5 OD 260/230 = 0.5-2.5 Txwv tsis pub lossis tsis muaj protein ntau lossis DNA kis tau pom ntawm gel. | Nroj tsuag: RIN ≥6.5 Tsiaj: RIN≥7.0 5.0 ≥28S / 18S≥1.0; txwv los yog tsis muaj qhov nce siab |
Pom zoo coj mus kuaj
Ntim:
2 ml centrifuge raj (Tin foil tsis pom zoo)
Sample labeling: Pab pawg + replicate xws li A1, A2, A3;B1, B2, B3....
Kev xa khoom:
1.Dry-ice: Cov qauv yuav tsum tau ntim rau hauv hnab thiab muab faus rau hauv cov dej khov qhuav.
2.RNAstable raj: RNA cov qauv tuaj yeem qhuav hauv RNA stabilization raj (xws li RNAstable®) thiab xa mus rau hauv chav tsev kub.
Kev Pabcuam Ua Haujlwm Flow
Kev sim tsim
Qauv xa tuaj
RNA rho tawm
Kev tsim kho tsev qiv ntawv
Sequencing
Cov ntaub ntawv tsom xam
Kev pab cuam tom qab muag
Bioinformatics
RNA kev nthuav qhia nthuav dav
Differentially nthuav qhia cov noob
kev soj ntsuam ceRNA
Sib txawv qhia miRNAs thiab lwm yam RNAs
Tshawb nrhiav cov kev tshawb fawb nce qib uas tau pab los ntawm BMKGene' tag nrho cov kev pabcuam sau tseg los ntawm kev sau cov ntawv tshaj tawm.
Dai, Y. et al.(2022) 'Comprehensive expression profiles of mRNAs, lncRNAs and miRNAs in Kashin-Beck disease identified by RNA-sequencing', Molecular Omics, 18(2), pp. 154–166.doi: 10.1039/D1MO00370D.
Liu, N. Nan et al.(2022) 'Txoj kev tshawb xyuas tag nrho ntev ntawm kev tiv thaiv khaub thuas ntawm Apis cerana hauv Changbai Roob thaum lub caij ntuj sov.', Gene, 830, pp. 146503–146503.doi: 10.1016/J.GENE.2022.146503.
Wang, XJ et al.(2022) 'Multi-Omics Integration-Based Prioritization of Competing Endogenous RNA Regulation Networks in Small Cell Lung Cancer: Molecular Characteristics and Drug Candidates', Frontiers in Oncology, 12, p.904865. doi: 10.3389/FONC.2022.904865/BIBTEX.
Xu, P. et al.(2022) 'Integrated analysis of the lncRNA/circRNA-miRNA-mRNA expression profiles nthuav tawm cov kev nkag siab tshiab rau cov txheej txheem muaj peev xwm teb rau cov hauv paus-knot nematodes hauv txiv laum huab xeeb', BMC Genomics, 23(1), pp. 1-12.ib: 10.1186/S12864-022-08470-3/FIGURES/7.
Yan, Z. et al.(2022) 'Txhua-transcriptome RNA sequencing qhia txog cov txheej txheem molecular cuam tshuam nrog kev saib xyuas tom qab sau qoob loo zoo hauv broccoli los ntawm liab LED irradiation', Postharvest Biology thiab Technology, 188, p.111878. doi: 10.1016/J.POSTHARVBIO.2022.111878.
