Usoro njegharị nje na fungal dum Genome
Bacterial na fungal dum-genome re-sequencing oru ngo dị mkpa maka ịga n'ihu microbial genomics site na-enyere mmecha na ntụnyere nke microbial genomes. Nke a na-eme ka injinia sie ike, njikarịcha usoro mmepụta ihe, yana nyocha nke ụzọ metabolism nke abụọ. Ọzọkwa, usoro nke fungal na nje bacteria dị oke mkpa maka ịghọta mgbanwe gburugburu ebe obibi, na-eme ka nsogbu dị mma, na ikpughe mgbanwe mkpụrụ ndụ ihe nketa, na-enwe mmetụta sara mbara na nkà mmụta ọgwụ, ọrụ ugbo, na sayensị gburugburu ebe obibi.
Uru ọrụ
●Nyocha bioinformatic gụnyere ọkpụkpọ dị iche iche:Inye nghọta na-arụ ọrụ n'ime genome ndị emegharịrị ọzọ.
● Ọkachamara dị ukwuu: Site na puku kwuru puku microbial re-sequencing oru ngo a na-eme kwa afọ, anyị na-eweta ihe karịrị afọ iri nke ahụmahụ, a ukwuu nkà analysis otu, keukwu ọdịnaya, na magburu onwe post-ire nkwado.
●Nkwado mgbe ọrịre ahịa:Nkwenye anyị ga-agafe karịa mmecha ọrụ yana oge ọrụ ọnwa 3 gachara. N'ime oge a, anyị na-enye nlebanya ọrụ, enyemaka nchọpụta nsogbu, yana oge ajụjụ & A iji zaa ajụjụ ọ bụla metụtara nsonaazụ ya.
Nkọwapụta ọrụ
| Usoro ikpo okwu | Usoro usoro | akwadoro data | njikwa mma |
| Illumina NovaSeq | PE150 | Omimi nke 100x | Q30≥85% |
Ihe atụ chọrọ
| Ntinye uche (ng/µL) | Ngụkọta ego (ng) | Olu (µL) |
| ≥1 | ≥60 | ≥20 |
Ihe nje bacteria: ≥1x107 mkpụrụ ndụ
Fungi Unicellular: ≥5x106-1 x107 mkpụrụ ndụ
Micro fungi: ≥4g
Usoro ọrụ ọrụ
Nbufe ihe atụ
Ịrụ ụlọ akwụkwọ
Usoro
Nyocha data
Mgbe-ire ọrụ
Gụnyere nyocha ndị a:
- Usoro njikwa ogo data
- Ndozi na genome ntụaka
- Oku dị iche iche: SNP na InDel
- Nkọwa dị iche iche
Oku dị iche iche: Ụdị SNP
Oku dị iche iche: Nkesa ogologo InDel
Nyochaa ọganihu ndị BMKGene's microbial genome re-sequencing ọrụ na-akwado site na nchịkọta mbipụta echekwabara.
Jia, Y. et al. (2023) 'Ịjikọta transcriptome na dum genome re-sequencing na ihuenyo ọrịa mgbochi mkpụrụ ndụ maka Wheat Dwarf Bunt',Akwụkwọ akụkọ ụwa nke sayensị molecular, 24 (24). doi: 10.3390/IJMS242417356.
Jiang, M. et al. (2023) 'Metabolism nke glucose na-achịkwa Ampicillin na-emegharị mgbanwe site na ndidi ruo nguzogide nje bacteria',Ọganihu Sayensị, 9 (10). doi: 10.1126/SCIADV.ADE8582/SUPPL_FILE/SCIADV.ADE8582_SM.PDF.
Yang, M. et al. (2022) 'Aliidiomarina halalaliphila sp. nov., nje nje haloalkaliphilic dịpụrụ adịpụ na ọdọ mmiri soda na Inner Mongolia Autonomous Region, China', Int. J. Sistemụ Evol.Microbiol, 72, p. 5263. doi: 10.1099/ijsem.0.005263.
Zhu, Z., Wu, R. na Wang, G.-H. (2024) 'usoro genome nke Staphylococcus nepalensis ZZ-2023a, dịpụrụ adịpụ na Nasonia vitripennis',Nkwuputa akụrụngwa Microbiology. doi: 10.1128/MRA.00802-23.
