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ọkọlọtọ-03

Ngwaahịa

Illumina na BGI

Teknụzụ usoro nke Illumina, dabere na Sequencing site Synthesis (SBS), bụ ihe ọhụrụ NGS nabatara n'ụwa niile, na-ahụ maka imepụta ihe karịrị 90% nke data usoro usoro ụwa.Ụkpụrụ nke SBS na-agụnye ịse foto n'ụdị fluorescent nke a na-akpọ reversible terminators ka agbakwunyere dNTP ọ bụla, ma mesịa kewaa iji kwe ka ntinye nke ntọala na-esote.N'ebe dNTP anọ niile na-atụgharị akwụsị akwụsị dị na usoro usoro nke ọ bụla, asọmpi eke na-ebelata mbuso agha.Teknụzụ a na-agbanwe agbanwe na-akwado ma ụlọ akwụkwọ ọbá akwụkwọ na-agụ otu na nke jikọtara ọnụ, na-enye ọtụtụ ngwa genomic.Ikike dị elu nke Illumina sequencing na idobe ya dị ka isi nkuku na nyocha nke genomics, na-enye ndị ọkà mmụta sayensị ike ka ha kpughee mgbagwoju anya nke genome nwere nkọwa na arụmọrụ enweghị atụ.

DNBSEQ, nke BGI mebere, bụ teknụzụ NGS ọzọ na-emepe emepe nke jisiri ike belata n'ihu ọnụ ahịa usoro na ịbawanye mmepụta.Nkwadebe nke ọba akwụkwọ DNBSEQ gụnyere nkewa DNA, nkwadebe nke ssDNA na mmụba okirikiri iji nweta DNA nanoballs (DNB).A na-ebunye ihe ndị a n'elu elu siri ike ma mechaa soro ya site na ngwakọta Probe-Anchor Synthesis (cPAS).

Ọrụ usoro ọba akwụkwọ anyị emeburu na-enyere ndị ahịa aka ịkwado ọba akwụkwọ ha si n'ụdị dị iche iche (mRNA, genome dum, amplicon, na ndị ọzọ).N'ikpeazụ, enwere ike ibuga ọba akwụkwọ ndị a gaa na ebe a na-eme usoro anyị maka njikwa mma na usoro n'usoro Illumina ma ọ bụ BGI.


Nkọwa ọrụ

Nsonaazụ ngosi

Atụmatụ

Platform:Illumina NovaSeq 6000, NovaSeq, HiSeq X iri na BGI-DNB-T7

Ụdị usoro:PE50, PE100, PE150, PE250

Njikwa ogo nke ọba akwụkwọ tupu usoro

Usoro nnyefe data na QC:nnyefe nke akụkọ QC na data raw na usoro fastq mgbe demultiplexing na nzacha Q30 na-agụ.

 

Uru ọrụ

Ọdịiche nke ọrụ usoro:onye ahịa nwere ike họrọ usoro site n'okporo ụzọ, cell flow ma ọ bụ ọnụọgụ data.

Mgbanwe nke nyiwe:Enwere ike ibufe ọba akwụkwọ DNB na nyiwe Illumina

Ahụmahụ dị ukwuu na ikpo okwu usoro usoro Illumina:na ọtụtụ puku ọrụ mechiri emechi nwere ụdị dị iche iche. 

Mbufe nke usoro QC akụkọ:ya na metrik dị mma, izi ezi data na ịrụ ọrụ n'ozuzu nke usoro nhazi.

Usoro usoro nke tozuru oke:na obere oge mgbanwe.

Njikwa ogo siri ike: anyị na-emejuputa iwu QC siri ike iji kwe nkwa ịnye nsonaazụ dị elu mgbe niile.

Ihe atụ chọrọ*

Usoro akụkụ akụkụ

Ọnụ ego data (X)

Ntinye uche (qPCR/nM)

Olu

X ≤ 50 Gb

≥2 nM

≥ 20 μl

50 Gb ≤ X <100 Gb

≥3 nM

≥ 20 μl

X ≥ 100 Gb

≥4 nM

≥ 20 μl

Otu ụzọ (Ilumina)

Platform

Ntinye uche (qPCR/nM)

Olu

HiSeq X iri

≥2 nM

≥ 20 μl

NovaSeq 6000 SP

≥1 nM

≥ 25 μl

NovaSeq 6000 S4

≥ 1.5 nM

≥ 25 μl

NovaSeq X

≥ 1.5 nM

≥ 25 μl

BGI-DNBSEQ-T7

≥ 1.5 nM

≥ 25 μl

 Na mgbakwunye na itinye uche na mkpokọta ego, a chọkwara ụkpụrụ kacha elu kwesịrị ekwesị.

 

Biko kpọtụrụ anyị ma ọ bụrụ na nlele gị emezughị ihe mmalite ihe achọrọ.

Usoro ọrụ ọrụ

nkwadebe sample

Njikwa ogo ụlọ akwụkwọ

Usoro

Usoro

Nyocha data

Njikwa ogo data

Ihe atụ QC

Nnyefe oru ngo


  • Nke gara aga:
  • Osote:

  • Akụkọ ụlọ akwụkwọ QC

    A na-enye akụkọ maka ogo ụlọ akwụkwọ ahụ tupu usoro nhazi, nyochaa ego ọbá akwụkwọ, na nkewa.

     

    Usoro nke akụkọ QC

     

    Tebụl 1. Statistics na usoro data.

    NJ nlele

    BMKID

    Raw na-agụ

    Data raw (bp)

    Ọgụgụ dị ọcha (%)

    Q20(%)

    Q30(%)

    GC(%)

    C_01

    BMK_01

    22,870,120

    6,861,036,000

    96.48

    99.14

    94.85

    36.67

    C_02

    BMK_02

    14,717,867

    4,415,360,100

    96.00

    98.95

    93.89

    37.08

    Ọgụgụ 1. Ogo nkesa tinyere na-agụ na nke ọ bụla sample

    A9

    Ọgụgụ 2. Isi nkesa ọdịnaya

    A10

    Ọgụgụ 3. Nkesa nke ọdịnaya agụ na usoro data

    A11

     

    nweta ntinye okwu

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