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Usoro mgbochi mgbochi Chromatin (ChiP-seq)
ChIP-Seq na-enye nkọwapụta genome-obosara nke ebumnuche DNA maka mgbanwe histone, ihe odide ederede, na protein ndị ọzọ metụtara DNA.Ọ na-ejikọta nhọrọ nke chromatin immuno-precipitation (ChiIP) iji nwetaghachi ogige protein-DNA kpọmkwem, na ike nke ọgbọ na-esote (NGS) maka usoro ntinye dị elu nke DNA enwetara.Na mgbakwunye, n'ihi na a na-anata ogige protein-DNA site na mkpụrụ ndụ dị ndụ, enwere ike iji saịtị njikọ n'ụdị cell na anụ ahụ dị iche iche, ma ọ bụ n'okpuru ọnọdụ dị iche iche.Ngwa sitere na ụkpụrụ ntụgharị gaa na ụzọ mmepe ruo na usoro ọrịa na karịa.
Platform: Illumina NovaSeq 6000
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Usoro genome bisulfite dum
DNA methylation na ọnọdụ nke ise na cytosine (5-mC) nwere mmetụta dị mkpa na mkpụrụ ndụ ihe nketa na ọrụ cellular.Ejikọtala ụkpụrụ methylation na-adịghị mma na ọnọdụ dị iche iche na ọrịa, dị ka ọrịa kansa.WGBS abụrụla ọkọlọtọ ọla edo maka ịmụ methylation genome-wide n'otu mkpebi ntọala.
Platform: Illumina NovaSeq6000
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Nyocha maka ntụgharị-Chromatin nwere ike ịnweta ya na usoro ntinye dị elu (ATAC-seq)
ATAC-seq bụ usoro nhazi nke dị elu maka nyocha nke nnweta chromatin genome, nke dị mkpa maka njikwa epigenetic zuru ụwa ọnụ nke mkpụrụ ndụ ihe nketa.A na-etinye ihe nkwụnye usoro n'ime mpaghara chromatin mepere emepe site na transposase Tn5 hyperactive.Mgbe nkwalite PCR gasịrị, a na-arụ ọba akwụkwọ nke usoro.Enwere ike nweta mpaghara chromatin niile mepere emepe n'okpuru ọnọdụ oge-oge, ọ bụghị naanị na saịtị njikọ nke ihe odide ederede, ma ọ bụ mpaghara ụfọdụ agbanwegharịrị histone.
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Mbelata Nnọchiteanya Bisulfite Usoro (RRBS)
Nchọpụta methylation DNA na-abụkarị isiokwu na-ekpo ọkụ na nyocha ọrịa, yana njikọ chiri anya na okwu mkpụrụ ndụ ihe nketa na ụdị pheno.RRBS bụ usoro ziri ezi, dị mma na nke akụ na ụba maka nyocha DNA methylation.Ịba ụba nke mpaghara ndị na-akwalite na CpG agwaetiti site na enzymatic cleavage (Msp I), jikọtara ya na usoro Bisulfite, na-enye nchọpụta DNA methylation dị elu.
Platform: Illumina NovaSeq 6000
