TGuide Smart Blood/Sell/Tissue RNA Kit
Nọmba katalọgụ / nkwakọ ngwaahịa
| Nwamba. mba | ID | Mba nke preps |
| 4995043 (efere) | DP661-E | 96 |
| 4993551 (katrij) | DP661-DE | 48 |
Ntuziaka nhọrọ reagent maka TGuide S16
| Nuclei acid | Ụdị Nlereanya | Aha ngwaahịa | Nha mbukota (preps) Katrij / efere | Nwamba mba. Katrij / efere |
| DNA | Anụ anụ | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Osisi & Mkpụrụ | TGuide Smart Igwe Ọdụdọ Igwe DNA | 48 | 4993548 |
| DNA | Ala/Stool | TGuide Smart Ala / Stool DNA Kit | 48 | 4993549 |
| DNA | Gel & PCR ngwaahịa | Kit DNA dị ọcha TGuide | 48 | 4993550 |
| DNA | Ọbara dum | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Cell/Swab/Ebe akọrọ, wdg | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Ọbara/Mkpụrụ ndụ/anụ ahụ | TGuide Smart Blood/Sell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Osisi & Mkpụrụ | TGuide Smart Igwe Ọdụdọ Igwe Ọdụdọ RNA | 48 | 4993552 |
| DNA/RNA | Fluids efu cell (Serum, wdg) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Ntinye ngwaahịa
Sachapụ mkpụrụ ndụ dị elu, ịdị ọcha dị elu, mkpokọta RNA dị elu site na ọbara/sel/anụ ahụ.
Enweghị reagents na-egbu egbu dị ka phenol/chloroform nwere
RNA enwetara nwere ịdị ọcha dị elu ma enwere ike iji ya ozugbo maka nchọpụta mgbawa, usoro ntinye dị elu na nnwale ndị ọzọ.
Atụmatụ
Dị mfe iji
Enweghị ọrụ pipụ ọzọ.
Enwere ike gbanwee olu elution dị ka achọrọ.
Nwụnye na ọrụ chọrọ obere ọzụzụ. Site na mmemme etinyegoro mbụ, bupụ katrijị ahụ, họrọ protocol wee mee nnwale gị. Mmemme dị njikere iji, yana enwere ike ịhazi ya.
Ebugoro reagents yana ngwa mkpofu dabara adaba.
A na-ekpochapụ njikwa kemịkalụ ruo oke kachasị iji hụ na nchekwa ọrụ ahụ.
Nsonaazụ ngosi
Ejiri Agilent 2100 mee nchọpụta iguzosi ike n'ezi ihe.
Isi mmalite: CD-1 ụmụ oke
Ọnọdụ nlele: ọbara zuru oke
Nlele nha: 200 μl
Nsonaazụ nnwale: mkpokọta RNA ewepụtara na ọbara nke TGuide S16 nwere reagent 4993551 nwere mkpụrụ dị mma na ịdị ọcha dị elu, ma nwee ike gboo mkpa nke ala.
usoro ihe dị elu.
Isi mmalite: CD-1 ụmụ oke
Ibu ibu: 1 μl. A na-agbaze imeju ugboro 5 ma buru ibu.
Ntinye uche Agarose gel: 1%. Electrophoresis maka 20 min na 6 V / cm;
M:TIANGEN Marker III 1-4: obi 5-8:
imeju 9-12: akpa ume 13-16: akụrụ
E weputara ihe nlele abụọ mbụ nke anụ ahụ ọ bụla site na ngwa mwepụta nke dabere na kọlụm, na TGuide S16 weputara abụọ ikpeazụ na-akpaghị aka.
Nsonaazụ nnwale: mkpokọta anụ ahụ RNA nke TGuide S16 weputara na-akpaghị aka nke nwere reagent 4993551 nwere mkpụrụ dị elu na ịdị ọcha, nke dabara na RNA nke usoro mmịpụta dabere na kọlụm weputara. Ịdị ọcha bụ otu ihe ahụ, yana OD260 / OD280 gburugburu 2.0 ~ 2.1 na OD260 / OD230> 2.0. Na mmechi, enwere ike iji TGuide S16 wepụta mkpokọta RNA site na anụ ahụ anụmanụ iji dochie ngwọta mmịpụta dabere na kọlụm.
