Ogologo usoro enweghị koodu-Ilumina
Uru ọrụ
●Nyocha nkwonkwo nke mRNA na lncRNA: site na ijikọta ọnụọgụ nke mRNA transcript na ọmụmụ nke lncRNA na ebumnuche ha, ọ ga-ekwe omume ịnweta nkọwa miri emi nke usoro nhazi nke na-akpata nzaghachi cellular.
●Ọkachamara sara mbara: Ndị otu anyị na-eweta ahụmịhe bara ụba na ọrụ ọ bụla, yana ndekọ ndekọ nke nhazi ihe karịrị 23,000 sample na BMK na-agbasa ụdị nlele dị iche iche na ọrụ lncRNA.
●Njikwa ogo siri ike: Anyị na-emejuputa isi ihe nchịkwa n'ofe niile, site na nlele na nkwadebe ọbá akwụkwọ ruo usoro na bioinformatics. Nleba anya nke ọma a na-eme ka o doo anya na nnyefe nke nsonaazụ dị elu mgbe niile.
●Nkwado maka ire ere: Nkwenye anyị na-agafe karịa mmecha ọrụ yana oge ọrụ ọnwa 3 gachara. N'ime oge a, anyị na-enye nlebanya ọrụ, enyemaka nchọpụta nsogbu, yana oge ajụjụ & A iji zaa ajụjụ ọ bụla metụtara nsonaazụ ya.
Ihe nlele chọrọ na nnyefe
| Ụlọ akwụkwọ | Platform | Data akwadoro | Data QC |
| ọba akwụkwọ ntụzịaka rRNA mebiela | Ilumina PE150 | 10-16 Gb | Q30≥85% |
Nucleotides:
| Conc.(ng/μl) | Ọnụ ego (μg) | Ịdị ọcha | Iguzosi ike n'ezi ihe |
| ≥ 80 | 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Enwere oke ma ọ bụ enweghị protein ma ọ bụ mmetọ DNA nke egosiri na gel. | RIN≥6.0; 5.0≥28S/18S≥1.0; nwere oke ma ọ bụ enweghị mgbago elu |
● Osisi:
Mgbọrọgwụ, azuokokoosisi ma ọ bụ petal: 450 mg
Akwụkwọ ma ọ bụ mkpụrụ: 300 mg
Mkpụrụ: 1.2 g
● Anụmanụ:
Obi ma ọ bụ eriri afọ: 450 mg
Viscera ma ọ bụ ụbụrụ: 240 mg
Akwara: 600 mg
Ọkpụkpụ, ntutu ma ọ bụ akpụkpọ: 1.5g
● Arthropods:
Ụmụ ahụhụ: 9g
Crustacea: 450 mg
● Ọbara dum:2 ọkpọkọ
● Selụ: 106 mkpụrụ ndụ
● Serum na Plasmammiri: 6 ml
Nnyefe nlele akwadoro
Akpa: 2 ml centrifuge tube (Tin foil adịghị akwadoro)
Ịkpọ aha nlele: Otu+ megharịa dịka A1, A2, A3; B1, B2, B3.
Mbupu:
1. Dry-ice: A ga-etinye ihe nlele n'ime akpa ma lie ya na ice-akọrọ.
2. tubes RNAstable: Enwere ike ịkpọsa ihe nlele RNA na tube stabilization RNA (dịka ọmụmaatụ RNAstable®) ma bufee ya n'ime ụlọ okpomọkụ.
Usoro ọrụ ọrụ
Nnwale imewe
Nbufe ihe atụ
mmịpụta RNA
Ịrụ ụlọ akwụkwọ
Usoro
Nyocha data
Mgbe-ire ọrụ
Bioinformatics
Nkọwapụta Gene dị iche iche (DEGs) nyocha
Nkọwa okwu lncRNA - nchịkọta
Nkwalite mkpụrụ ndụ ihe nketa lncRNA
MRNA nkwonkwo na nyocha ọnọdụ lncRNA - atụmatụ Circos (okirikiri etiti bụ mRNA na ime ime bụ lncRNA)
Nyochaa ọganihu ndị BMKGene's lncRNA equencing na-akwado site na nchịkọta mbipụta.
Ji, H. et al. (2020) 'Nchọpụta, amụma arụ ọrụ, na isi nncRNA nkwenye nke lncRNA nwere nsogbu oyi na imeju oke', Akụkọ Sayensị 2020 10:1, 10(1), p. 1-14. doi: 10.1038/s41598-020-57451-7.
Jia, Z. et al. (2021) 'Ntụle ntụgharị ederede jikọtara ọnụ na-ekpughe usoro mgbochi ọrịa maka eriri Carp nkịtị nke CyHV-3', Frontiers in Immunology, 12, p. 687151. doi: 10.3389/FIMMU.2021.687151/BIBTEX.
Wang, XJ, et al. (2022) 'Multi-Omics Integration-based Prioritization of Enogenous RNA Regulation Networks in Small Cell Lung Cancer: Molecular Characteristics and Drug Candidates', Frontiers in Oncology, 12, p. 904865. doi: 10.3389/FONC.2022.904865/BIBTEX.
Xiao, L. et al. (2020) 'Genetic dissection nke gene coexpression netwọk n'okpuru photosynthesis na Populus', Plant Biotechnology Journal, 18(4), p. 1015-1026. doi: 10.1111/PBI.13270.
Zheng, H. et al. (2022) 'Network Regulatory Network for Dysregulated Gene Expression and Abnormal Metabolic Signaling in Immune Cells in Micro Environment of Graves' Disease and Hashimoto's Thyroiditis', Frontiers in Immunology, 13, p. 879824. doi: 10.3389/FIMMU.2022.879824/BIBTEX.
