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ọkọlọtọ-03

Ngwaahịa

Metabolomics

Metabolome bụ ihe dị n'akụkụ ala nke genome na mejupụtara mkpokọta mkpokọta ụmụ irighiri ihe dị obere (metabolites) dị na cell, anụ ahụ, ma ọ bụ organism.Metabolomics bu n'obi tụọ obosara obosara nke obere ụmụ irighiri ihe n'ihe gbasara mkpali physiological ma ọ bụ steeti ọrịa.Usoro metabolomics na-adaba n'otu abụọ dị iche iche: metabolomics na-abụghị nke ezubere iche, nyocha zuru oke nke nyocha niile enwere ike ime na nlele gụnyere kemịkalụ amaghi ama site na iji GC-MS/LC-MS, yana metabolomics ezubere iche, nha nke akọwapụtara otu kemịkalụ. metabolites biochemically annotated.


Nkọwa ọrụ

Ihe atụ chọrọ

Metabolism na-abụghị nke ezubere icheỤdị ihe atụ

Nlereanya akwadoroego

Nkwugharị ndu

LC-MS/GC-MSmetabolomics na-enweghị atụ

Anụ ahụ

200mg

Osisi ≥6;

Anụ anụmanụ ≥10;

Mmadụ ≥30;

Plasma/serum

200ul

Ọdịnaya Fecal/Intestinal

150mg

Selụ

1*106

mmamịrị

500ul

Mmiri mmiri

1 ml

Metabolomics ezubere icheỤdị ihe atụ

Ọnụ ego nlele akwadoro

Nkwugharị ndu

Phytohormone/Typtophane/Metabolism ike / Oxidized lipids / Carotenoid;carotinoid

Anụ ahụ

500mg

Osisi ≥3 ;

Anụ anụmanụ ≥6;

Plasma/serum

500ul

Fekal

1000mg

Selụ

1*107

Nkọwapụta ọrụ

Metabolomics na-abụghị nke ezubere iche:
1. Tozuru okè na ike technology, elu mkpebi na ezi selectivity
2. Ebe nchekwa data zuru ezu;
3. Kwesịrị ekwesị maka nyocha nke matrices dị mgbagwoju anya, na-enye ozi zuru ezu n'otu nyocha;

Metabolomics ezubere iche:
1.Jiri nkwenye ọkọlọtọ dị n'ime nke isotope iji meziwanye qualitative na quantitative nke ihe;
2.Quality njikwa usoro iji hụ na a pụrụ ịdabere na data.

Usoro ọrụ ọrụ

1. Metabolomics na-abụghị nke ezubere iche

11

2.Targeted metabolomics

22

3.Widely ezubere iche metabolomics

33

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