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Usoro Metatranscriptome

Metatranscriptome Sequencing egosipụtara

Iji teknụzụ usoro usoro Illumina, BMKGENE's metatranscriptome sequencing ọrụ na-ekpughere mkpụrụ ndụ ike dị ike nke ụdị dị iche iche nke ụmụ nje, na-agbasa eukaryotes na prokaryotes na nje, n'ime gburugburu eke dị ka ala, mmiri, oké osimiri, stool, na eriri afọ. Ọrụ zuru oke anyị na-enye ndị nyocha ike ịbanye n'ime profaịlụ mkpụrụ ndụ ihe nketa zuru oke nke obodo microbial. Na-agafe nyocha taxonomic, ọrụ usoro usoro metatranscriptome anyị na-eme ka nyocha n'ime ịba ụba ọrụ, na-eme ka ìhè dị na mkpụrụ ndụ ihe nketa egosipụtara dị iche iche na ọrụ ha. Choputa otutu ihe nleba anya nke ndu ka ị na-agagharị n'ọdịdị mgbagwoju anya nke okwu mkpụrụ ndụ ihe nketa, ụdị taxonomic dị iche iche, na arụrụ ọrụ n'ime niche gburugburu ebe obibi ndị a dị iche iche.

Kpọtụrụ anyị

Nkọwa ọrụ

Bioinformatics

Nsonaazụ ngosi

Akwụkwọ ndị egosipụtara

Atụmatụ ọrụ

● Mbelata rRNA na-esochi nkwadebe ọbá akwụkwọ mRNA ntụziaka.

● Usoro na Illumina NovaSeq.

Uru ọrụ

●Mụta mgbanwe nke obodo Microbial mgbagwoju anya:Nke a na-eme na ọkwa ederede wee chọpụta mkpụrụ ndụ ihe nketa ọhụrụ nwere ike ime.

●Na-akọwa mmekọrịta Microbial Community na onye ọbịa ma ọ bụ gburugburu.

●Nyocha bioinformatic zuru oke: Nke a na-enye nghọta na obodo taxonomic na arụ ọrụ mejupụtara, yana dị iche iche mkpụrụ ndụ okwu analysis.

●Nkọwa mkpụrụ ndụ dị ukwuu:Iji nchekwa data arụ ọrụ mkpụrụ ndụ ihe ọhụrụ maka ozi nkwupụta mkpụrụ ndụ ihe nketa nke obodo microbial.

●Nkwado mgbe ọrịre ahịa:Nkwenye anyị ga-agafe karịa mmecha ọrụ yana oge ọrụ ọnwa 3 gachara. N'ime oge a, anyị na-enye nlebanya ọrụ, enyemaka nchọpụta nsogbu, yana oge ajụjụ & A iji zaa ajụjụ ọ bụla metụtara nsonaazụ ya.

Nkọwapụta ọrụ

Usoro ikpo okwu	Usoro usoro	akwadoro data	Njikwa ogo data
Illumina NovaSeq	PE150	12Gb	Q30≥85%

Ihe atụ chọrọ

Ntinye uche (ng/µL)	Ngụkọta ego (µg)	Olu (µL)	OD260/280	OD260/230	RIN
≥50	≥1.0	≥20	1.8-2.0	1.0-2.5	≥6.5

Usoro ọrụ ọrụ

Nbufe ihe atụ

Ịrụ ụlọ akwụkwọ

Usoro

Nyocha data

Mgbe-ire ọrụ

Nke gara aga: De novo Fungal Genome Assembly

Osote: Usoro prokaryotic RNA

Ọ gụnyere nyocha ndị a:

● Usoro njikwa ogo data

● Mgbakọ transcript

● Nkọwa Taxonomic na ụbara

● Nkọwa ọrụ na ụbara

● Nkọwapụta okwu na nyocha dị iche iche

Nkesa taxonomic nke nlele ọ bụla:

Nyocha ụdị dị iche iche beta: UPGMA

Nkọwa ọrụ - Gaa n'ụba

Ụba taxonomy dị iche iche - LEFSE

Nyochaa ọganihu ndị BMKGene meta transcriptomics sequencing ọrụ na-akwado site na nchịkọta mbipụta echekwabara.

Lu, Z. et al. (2023) 'Nnagide acid nke nje bacteria na-eji lactate n'usoro Bacteroidales na-enye aka na mgbochi nke ruminal acidosis n'ime ewu na-eme ka nri na-etinye uche dị elu',Nri anụmanụ, 14, peeji 130–140 . doi: 10.1016/J.ANINU.2023.05.006.

Abụ, Z. et al. (2017) 'Na-ewepụta microbiota na-arụ ọrụ nke ọma na gbaa ụka steeti siri ike site na amplicon dị elu na usoro metatranscriptomics',Ọganihu na Microbiology, 8 (Jul). doi: 10.3389/FMICB.2017.01294/FULL.

Wang, W. et al. (2022) 'Achọpụtara Mycoviruses Novel site na nyocha Metatranscriptomics nke Phytopathogenic Alternaria Fungus',Nje Virus, 14 (11), p. 2552. doi: 10.3390/V14112552/S1.

Wei, J. et al. (2022) 'Ntụle metatranscriptome yiri ya na-ekpughe mmebi nke metabolites nke abụọ nke osisi site na beet na eriri afọ ha',Molecular Ecology, 31 (15), p. 3999–4016. doi: 10.1111/MEC.16557.