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ọkọlọtọ-03

Ngwaahịa

Usoro Metatranscriptome

Metatranscriptome sequencing na-achọpụta mkpụrụ ndụ ihe nketa nke ụmụ nje (ma eukaryotes na prokaryotes) n'ime gburugburu ebe obibi (ya bụ ala, mmiri, oke osimiri, nsị, na eriri afọ) .Kpọmkwem, ọrụ a na-enye gị ohere ịnweta nkọwapụta mkpụrụ ndụ dum nke obodo microbial dị mgbagwoju anya, nyocha taxonomic. ụdị dị iche iche, nyocha nkwalite ọrụ nke mkpụrụ ndụ ihe nketa dị iche iche, na ndị ọzọ.

Platform: Illumina NovaSeq Platform


Nkọwa ọrụ

Ihe atụ chọrọ

Ụdị ihe atụ: mkpokọta RNA;

Ọnụ ego: 2.5ug;

Ntinye uche:50ng/ul;

Atụmatụ ọrụ

Ụdị ọba akwụkwọ: 250-300bp tinye cDNA ọba akwụkwọ;

Usoro nhazi usoro na atụmatụ: illumina NovaSeq Platform;paired-end 150bp;

Mmepụta data akwadoro: 12G raw data/ sample

Usoro ọrụ ọrụ

nnyefe sample

Nbufe ihe atụ

Nkwadebe ụlọ akwụkwọ

Ịrụ ụlọ akwụkwọ

Usoro

Usoro

Nyocha data

Nyocha data

Mgbe ire Ọrụ

Mgbe-ire ọrụ


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