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Usoro metagenomic (NGS)
Metagenome na-ezo aka mkpokọta ihe mkpụrụ ndụ ihe nketa nke obodo agwakọta dị ka metagenome gburugburu ebe obibi, metagenome mmadụ, wdg.Usoro metagenomic bụ ngwá ọrụ molekụla eji enyocha ihe agwakọta genomic nke ewepụtara site na nlele gburugburu ebe obibi, nke na-enye ozi zuru ezu na ụdị dị iche iche na ụbara, nhazi ọnụ ọgụgụ mmadụ, mmekọrịta phylogenetic, mkpụrụ ndụ ihe nketa na-arụ ọrụ na netwọk mmekọrịta yana ihe gburugburu ebe obibi.
Platform:Illumina NovaSeq6000
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Usoro Metagenomic-Nanopore
Metagenomics bụ ngwá ọrụ molekụla eji nyochaa ihe ngwakọta genomic nke ewepụtara site na ihe atụ gburugburu ebe obibi, nke na-enye ozi zuru ezu na ụdị dị iche iche na ụbara, nhazi ọnụ ọgụgụ mmadụ, mmekọrịta phylogenetic, mkpụrụ ndụ ihe nketa na-arụ ọrụ na netwọk mmekọrịta na ihe gburugburu ebe obibi, wdg. Nanopore usoro nyiwe ka ewebatala nso nso a. na ọmụmụ metagenomic.Ọrụ ya pụtara ìhè n'ogologo ọgụgụ na-akwalite nyocha nke metagenomic iyi, ọkachasị mgbakọ metagenome.N'iji uru nke ogologo ọgụgụ ọgụgụ, ọmụmụ metagenomic dabere na Nanopore na-enwe ike nweta mgbakọ na-aga n'ihu na-atụnyere metagenomics nke egbe.Ebipụtala ya na metagenomics dabere na Nanopore rụpụtara nke ọma na mkpụrụ ndụ nje mechiri emechi site na microbiomes (Moss, EL, et. al,Nature Biotech, 2020)
Platform:Nanopore PromethION P48
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16S/18S/ITS Amplicon Sequencing-PacBio
Nke a dị na 16S na 18S rRNA nwere mpaghara echekwara nke ukwuu yana hyper-variable bụ akara mkpisiaka mkpụrụ ndụ zuru oke maka njirimara prokaryotic na eukaryotic.Na-ewere uru nke sequencing, ndị a amplicons nwere ike ezubere iche dabere na chekwara akụkụ na hyper-variable mpaghara nwere ike n'ụzọ zuru ezu ji maka microbial njirimara na-enye aka ọmụmụ ekpuchi microbial diversity analysis, taxonomy, phylogeny, wdg Single-molekul ozugbo (SMRT) ) usoro nke PacBio ikpo okwu na-enyere aka inweta nke ukwuu ziri ezi ogologo agụ, nke nwere ike ikpuchi zuru-ogologo amplicon (ihe dị ka 1.5 Kb).Echiche sara mbara nke mkpụrụ ndụ ihe nketa mere ka mkpebi siri ike dị na nkọwapụta ụdịdị na nje bacteria ma ọ bụ fungi.
Platform:PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
16S/18S/ITS amplicon sequencing bu n'obi ikpughe phylogeny, taxonomy, na ụdị dị ukwuu n'ime obodo microbial site na nyocha ngwaahịa PCR nke ihe nrịbama mkpụrụ ndụ ihe nketa nke nwere ma akụkụ ndị nwere mkparịta ụka na hypervariable.Iwebata akara mkpisi aka molikula zuru oke site na Woeses et al, (1977) na-enye ikike profaịlụ microbiome na-enweghị ikewapụ.Usoro nke 16S (bacteria), 18S (fungi) na Internal transcripted spacer(ITS, fungi) na-enye ohere ka a mata ụdị ma ụdị dị ukwuu yana ụdị dị ụkọ na nke a na-amaghị ama.Teknụzụ a aghọwo ihe a na-etinye n'ọtụtụ ebe na ngwá ọrụ bụ isi n'ịchọpụta ihe mejupụtara microbial dị iche iche na gburugburu ebe dị iche iche, dị ka ọnụ mmadụ, eriri afọ, nsị, wdg.
Platform:Illumina NovaSeq6000
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Nhazigharị nje bacteria na fungal dum Genome
Bacterial na fungal dum genome re-sequencing bụ ngwá ọrụ dị oke mkpa iji mechaa genome nke nje bacteria na fungi a ma ama, yana iji tụnyere ọtụtụ genome ma ọ bụ iji map genomes nke ihe ọhụrụ dị ndụ.Ọ dị oke mkpa ịhazi usoro genome niile nke nje bacteria na fungi iji mepụta mkpụrụ ndụ ihe nketa ziri ezi, ime nchọpụta microbial na ọmụmụ ihe ọmụmụ genome ndị ọzọ.
Platform: Illumina NovaSeq 6000
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Fungal Genome
Teknụzụ Biomarker na-enye nyocha genome, ezigbo genome na genome zuru oke nke fungal dabere na ebumnuche nyocha akọwapụtara.Enwere ike nweta usoro genome, mgbakọ na nkọwa ọrụ site na ijikọta usoro nke ọgbọ na-abịa + usoro ọgbọ nke atọ iji nweta mgbakọ genome dị elu.Enwere ike iji teknụzụ Hi-C rụọ ọrụ iji kwado mgbakọ genome na ọkwa chromosome.
Platform:PacBio Sequel II
Nanopore PromethION P48
Illumina NovaSeq 6000
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Nje bacteria zuru ezu Genome
Teknụzụ Biomarker na-enye ọrụ nhazi usoro maka iwulite genome nke nje bacteria na enweghị oghere efu.Isi ọrụ nje bacteria na-arụ ọrụ genome zuru oke gụnyere usoro ọgbọ nke atọ, mgbakọ, nkọwa ọrụ na nyocha bioinformatic dị elu na-emezu ebumnuche nyocha.Nkọwapụta zuru oke nke genome nje bacteria na-enye ike ikpughe usoro ndị bụ isi na-adabere na usoro ndu ha, nke nwekwara ike inye nkọwa bara uru maka nchọpụta mkpụrụ ndụ ihe nketa na ụdị eukaryotic dị elu.
Platform:Nanopore PromethION P48 + Illumina NovaSeq 6000
PacBio Sequel II
