Ngwaahịa

Prokaryotic RNA usoro

Ihe onyonyo egosipụtara na usoro Prokaryotic RNA

Usoro prokaryotic RNA na-eji usoro ọgbọ na-abịa (NGS) iji kpughee ọnụnọ na ọnụọgụ RNA n'otu oge, site na nyocha transcriptome cellular na-agbanwe agbanwe.Usoro prokaryotic RNA nke ụlọ ọrụ anyị, ebumnuche kpọmkwem na prokaryotes nwere mkpụrụ ndụ ihe nketa, na-enye gị profaịlụ transcriptome, nyocha usoro mkpụrụ ndụ ihe nketa, wdg. etinyere ya n'ọtụtụ ebe na nyocha sayensị bụ isi, nyocha ọgwụ na mmepe, yana ndị ọzọ.

Platform: Illumina NovaSeq Platform

Kpọtụrụ anyị

Nkọwa ọrụ

Nkọwapụta ọrụ

Platform: Illumina NovaSeq ikpo okwu, PE150

Ụdị ọba akwụkwọ: 350bp tinye cDNA ọba akwụkwọ (dabere na nkesa kacha elu)

Usoro nhazi usoro: Ejikọtara ọnụ- Ọgwụgwụ 150 bp

Ọnụ ego data kwadoro: 2 Gb raw data/ sample

Bioinformatics

(1) Ịhazi njikwa njikwa data: Nkesa Nucleotide, Nkesa ogo ntọala, ntule nha rRNA;

(2) Ntụle nhazi usoro: Ndekọ ọnụ ọgụgụ nsonaazụ, saturation nke usoro, Mkpuchi nke usoro;

(3) Ntụaka genome nkọwa (NR, Swiss-Prot, Pfam, COG, GO, KEGG);

(4) Ntụle okwu: nkesa okwu (Na abụọ ma ọ bụ karịa sample), Nyocha nke okwu n'etiti sample (Venn analysis, Correlation analysis, PCA analysis);

(5) Nyocha okwu dị iche iche (Na ihe atụ abụọ ma ọ bụ karịa);

(6) Ntụle nhazi nke mkpụrụ ndụ: nyocha Venn, nyocha ụyọkọ, nyocha nkọwa ọrụ (COG, GO, KEGG), nyocha nkwalite ọrụ (GO, KEGG), eserese chordal na-arụ ọrụ, nyocha Ipath;

(7) nyocha sRNA: amụma sRNA, nkọwa sRNA (Blast, sRNAMap, sRNATarBase, SIPHT, Rfam), amụma nhazi nke abụọ sRNA, amụma mkpụrụ ndụ sRNA lekwasịrị anya;

(8) Ntụle nhazi ederede: nyocha nke operon, mmalite ederede na njedebe amụma saịtị, nkọwa UTR na nyocha, amụma usoro nkwalite, SNP/InDel nyocha (SNP/InDel annotation, SNP/InDel nkesa mpaghara, ọnụ ọgụgụ SNP).