Mbelata Nnọchiteanya Bisulfite Sequencing (RRBS)
Nkọwapụta ọrụ
Platform: NovaSeq ikpo okwu, PE150
Ụdị ọba akwụkwọ: 200-400bp tinye bisulfite mesoo ọbá akwụkwọ DNA
Usoro nhazi usoro: Ejikọtara ọnụ- Ọgwụgwụ 150 bp
Na-atụ aro mmepụta data: 10 Gb raw data/ sample
Ihe atụ chọrọ
Ọnụ ọgụgụ DNA: ≥ 2ug
Ntinye DNA:≥ 20ng/μl.
Ịdị ọcha: OD260/280 = 1.8 ruo 2.0 na-enweghị mmebi ma ọ bụ mmetọ RNA
Nyocha bioinformatics
Usoro ọrụ ọrụ
Nbufe ihe atụ
Ịrụ ụlọ akwụkwọ
Usoro
Nyocha data
Mgbe-ire ọrụ
a.Ntụleaka genome alignment statistics
Ọnụọgụ nke maapụ agụ
Sọnụ ọgụgụ nke usoro nhazi omimi na mkpuchi nke saịtị C
Akpokọtara mkpuchi
Digestion arụmọrụ nke ọ bụla sample
b.Nchọpụta saịtị methylation
Ndepụta nke ọkwa methylation na saịtị C
BNtughari S
Mcytosine ethylated
Nkesa ọkwa methylation
c.Map methylation
Genome-obosara
Gihe mmewere
d.Tụnyere ọkwa methylation n'etiti ihe atụ
PNjikọ ikuku nke CG methylation
PCA-CG methylation
Cmmetụta nke CG methylation
d.Nyocha methylation dị iche iche
SỌnụ ego nke DMR
Nkesa ogologo DMR
Hrie map
DNkesa MR na mpaghara ezubere iche
