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ọkọlọtọ-03

Ngwaahịa

Obere usoro RNA-Ilumina

Obere ụmụ irighiri RNA (sRNA), nke na-adịkarị n'okpuru 200 nucleotides n'ogologo, gụnyere microRNAs (miRNAs), obere RNA (siRNAs), na RNA ndị na-emekọrịta ihe (piRNAs).N'ime ndị a, miRNA, gburugburu 20-24 nucleotides ogologo, bụ ihe kwesịrị ịrịba ama maka ọrụ nchịkwa ha dị mkpa na usoro cellular dị iche iche.Site na usoro ngosipụta nke anụ ahụ na nke akọwapụtara nke ọma, miRNA na-egosipụta nchekwa dị elu n'ofe ụdị dị iche iche.

Platform: Illumina NovaSeq


Nkọwa ọrụ

Bioinformatics

Nsonaazụ ngosi

Akwụkwọ ndị egosipụtara

Atụmatụ

● Nha nhọrọ nke RNA tupu nkwadebe ọbá akwụkwọ

● Nyocha bioinformatic gbadoro ụkwụ na amụma miRNA na ebumnuche ha

Uru ọrụ

Nyocha bioinformatics zuru oke:na-eme ka njirimara nke miRNA ndị ama ama na nke ọhụrụ, njirimara nke ebumnuche miRNA na nkọwa ọrụ kwekọrọ na ịba ụba na ọtụtụ ọdụ data (KEGG, GO)

Njikwa ogo siri ike: anyị na-emejuputa isi ihe nchịkwa n'ofe niile, site na nlele na nkwadebe ọbá akwụkwọ ruo usoro na bioinformatics.Nleba anya nke ọma a na-eme ka o doo anya na nnyefe nke nsonaazụ dị elu mgbe niile.

Nkwado maka ire ere: Nkwenye anyị na-agafe karịa mmecha ọrụ yana oge ọrụ ọnwa 3 gachara.N'ime oge a, anyị na-enye nlebanya ọrụ, enyemaka nchọpụta nsogbu, yana oge ajụjụ & A iji zaa ajụjụ ọ bụla metụtara nsonaazụ ya.

Ọkachamara sara mbara: na ndekọ egwu nke imechi nke ọma n'ọtụtụ ọrụ sRNA na-ekpuchi ụdị 100 na ngalaba nyocha dị iche iche, ndị otu anyị na-eweta ahụmahụ bara ụba na ọrụ ọ bụla.

Ihe nlele chọrọ na nnyefe

Ụlọ akwụkwọ

Platform

Data akwadoro

Data QC

ahọpụtara nha

Illumina SE50

10M-20M na-agụ

Q30≥85%

Ihe atụ chọrọ:

Nucleotides:

Conc.(ng/μl)

Ọnụ ego (μg)

Ịdị ọcha

Iguzosi ike n'ezi ihe

≥ 80

≥ 0.5

OD260/280=1.7-2.5

OD260/230=0.5-2.5

Enwere oke ma ọ bụ enweghị protein ma ọ bụ mmetọ DNA nke egosiri na gel.

RIN≥6.5;

5.0≥28S/18S≥1.0;

nwere oke ma ọ bụ enweghị mgbago elu

● Osisi:

Mgbọrọgwụ, azuokokoosisi ma ọ bụ petal: 450 mg

Akwụkwọ ma ọ bụ mkpụrụ: 300 mg

Mkpụrụ: 1.2 g

● Anụmanụ:

Obi ma ọ bụ eriri afọ: 450 mg

Viscera ma ọ bụ ụbụrụ: 240 mg

Akwara: 600 mg

Ọkpụkpụ, ntutu ma ọ bụ akpụkpọ: 1.5g

● Arthropods:

Ụmụ ahụhụ: 9g

Crustacea: 450 mg

● Ọbara dum: 2 tube

● Selụ: 106 mkpụrụ ndụ

● Ọbara na Plasma:6 ml

Nnyefe nlele akwadoro

akpa:
2 ml nke centrifuge tube (anaghị akwado foil tin)
Ịkpọ aha nlele: Otu+ megharịa dịka A1, A2, A3;B1, B2, B3......

Mbupu:
1.Dry-ice: A ga-etinye ihe nlele n'ime akpa ma lie ya na akọrọ-ice.
2.RNAstable tubes: Enwere ike ihichapụ ihe nlele RNA na tube stabilization RNA (dịka RNAstable®) ma bufee ya na okpomọkụ.

Usoro ọrụ ọrụ

Ihe atụ QC

Nnwale imewe

nnyefe sample

Nbufe ihe atụ

Nnwale pilot

mmịpụta RNA

Nkwadebe ụlọ akwụkwọ

Ịrụ ụlọ akwụkwọ

Usoro

Usoro

Nyocha data

Nyocha data

Mgbe ire Ọrụ

Mgbe-ire ọrụ


  • Nke gara aga:
  • Osote:

  • Bioinformatics

    wps_doc_14

    Nchọpụta nke miRNA: nhazi na omimi

     

     

     miRNA-precursor-nhazi-na-usoro-omimi

     

    Okwu dị iche iche nke miRNA - nchịkọta ọkwa

    图片34

     

    Nkọwa ọrụ nke ebumnuche nke miRNA ndị egosipụtara iche

    图片35

    Nyochaa ọganihu nyocha nke ọrụ nhazi usoro BMKGene'sRNA na-akwado site na nchịkọta mbipụta.

      

    Chen, H. et al.(2023) 'ọrịa nje na-egbochi saponin biosynthesis na photosynthesis na Panax notoginseng', Plant Physiology and Biochemistry, 203, p.108038. doi: 10.1016/J.PLAPHY.2023.108038.

    Li, H. et al.(2023)' Osisi FYVE ngalaba-nwere protein FREE1 na-ejikọta ya na microprocessor components iji mebie miRNA biogenesis', EMBO na-akọ, 24(1).doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.

    Yu, J. et al.(2023) 'MicroRNA Ame-Bantam-3p na-achịkwa Larval Pupal Development site n'ịtụle Multiple Epidermal Growth Factor-like Domains 8 Gene (megf8) na Honeybee, Apis mellifera', International Journal of Molecular Sciences, 24(6), p. .5726. doi: 10.3390/IJMS24065726/S1.

    Zhang, M. et al.(2018) 'Ntụle agbakwunyere nke MiRNA na mkpụrụ ndụ ihe nketa jikọtara ya na ịdị mma anụ na-ekpughe na Gga-MiR-140-5p na-emetụta abụba anụ ahụ intramuscular na ọkụkọ', Physiology Cellular and Biochemistry, 46(6), p. 2421-2433.doi: 10.1159/000489649.

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