-
Usoro mRNA zuru oke-Nanopore
Usoro RNA abụrụla ngwa bara uru maka nyocha transcriptome zuru oke.Obi abụọ adịghị ya, usoro ọgụgụ dị mkpirikpi ọdịnala nwetara ọtụtụ mmepe dị mkpa na ebe a.Ka o sina dị, ọ na-ezutekarị mmachi na njirimara isoform ogologo zuru oke, nha, echiche PCR.
Usoro Nanopore na-ekewa onwe ya na usoro nhazi usoro ndị ọzọ, n'ihi na a na-agụ nucleotides ozugbo na-enweghị njikọ DNA ma na-emepụta ogologo oge na iri puku kilobases.Nke a na-enye ike ịgụ akwụkwọ ozugbo na-agafe transcript zuru oke yana imeri ihe ịma aka ndị dị na ọmụmụ ọkwa-isoform.
Platform:Ọnụ ego nke Nanopore PromethION
Ụlọ akwụkwọ:cDNA-PCR
-
De novo Usoro transcriptome ogologo zuru oke -PacBio
De novousoro transcriptome zuru oke, nke a makwaara dị kaDe novoIso-Seq na-ewere uru nke PacBio sequencer n'ogologo ọgụgụ, nke na-enyere usoro nke cDNA molekul zuru ezu na-enweghị nkwụsịtụ ọ bụla.Nke a na-ezere kpam kpam njehie ọ bụla ewepụtara na usoro mgbakọ transcript wee wulite unigene sets na mkpebi ọkwa isoform.Usoro unigene a na-enye ozi mkpụrụ ndụ ihe nketa siri ike dị ka “genome ntụaka” na ọkwa transcriptome.Na mgbakwunye, ijikọ na data usoro usoro ọgbọ na-abịa, ọrụ a na-enye ike nleba anya nke nkwupụta ọkwa isoform.
Platform: PacBio Sequel IIỌbá akwụkwọ: SMRT mgbịrịgba ọba akwụkwọ -
Eukaryotic mRNA usoro-Ilumina
usoro mRNA na-enyere aka ịkọwapụta mRNA niile edere site na sel n'okpuru ọnọdụ akọwapụtara.Ọ bụ teknụzụ siri ike maka ikpughe profaịlụ mkpụrụ ndụ ihe nketa, usoro mkpụrụ ndụ ihe nketa na usoro mkpụrụ ndụ nke ụfọdụ usoro ndu.Ruo ugbu a, usoro mRNA ejirila n'ọrụ n'ọtụtụ ebe na nyocha nyocha, nyocha ụlọ ọgwụ, mmepe ọgwụ, wdg.
Platform: Illumina NovaSeq 6000
-
Usoro mRNA na-abụghị nke nrụtụ aka-Ilumina
usoro mRNA na-anabata usoro usoro usoro ọgbọ na-abịa (NGS) iji weghara onye ozi RNA (mRNA) na-etolite Eukaryote n'oge akọwapụtara nke ụfọdụ ọrụ pụrụ iche na-arụ ọrụ.A na-akpọ transcript kachasị ogologo nke spliced 'Unigene' wee jiri ya dị ka usoro ntụaka maka nyocha na-esote, nke bụ ụzọ dị irè iji mụọ usoro molekụla na netwọk nchịkwa nke ụdị na-enweghị ntụle.
Mgbe mgbakọ data transcriptome na nkọwa ọrụ unigene gasịrị
(1) A ga-ahazi nyocha SNP, nyocha SSR, amụma CDS na usoro mkpụrụ ndụ ihe nketa.
(2) A ga-eme ọnụọgụ nke okwu unigene na nlele ọ bụla.
(3) A ga-achọpụta unigenes egosipụtara dị iche iche n'etiti nlele (ma ọ bụ otu) dabere na nkwupụta unigene.
(4) A ga-eme nchịkọta, nkọwa ọrụ na nyocha ịba ụba nke unigenes dị iche iche.
-
Ogologo usoro enweghị koodu-Ilumina
Ogologo RNA ndị na-abụghị koodu (lncRNAs) bụ ụdị mkpụrụ ndụ RNA nwere ogologo karịa 200 nt, bụ nke ejiri ikike nzuzo dị ala mara.LncRNA, dị ka onye bụ isi na RNA ndị na-abụghị koodu, bụ nke a na-ahụ ya na oghere na plasma.Mmepe nke usoro teknụzụ na bioinfortics na-enyere aka ịchọpụta ọtụtụ lncRNA ọhụrụ ma jikọta ndị nwere ọrụ ndu.Ihe akaebe gbasara mkpokọta na-egosi na lncRNA na-etinye aka na nhazi epigenetic, ụkpụrụ ndegharị na ụkpụrụ post-transcription.
-
Obere usoro RNA-Ilumina
Obere RNA na-ezo aka na klaasị nke ụmụ irighiri ihe RNA na-anaghị etinye koodu nke na-adịkarị obere karịa 200nt n'ogologo, gụnyere micro RNA (miRNA), obere nnyonye anya RNA (siRNA), na RNA (piRNA) na-emekọrịta ihe.
MicroRNA (miRNA) bụ klaasị nke obere RNA endogenous nwere ogologo ihe dịka 20-24nt, nke na-arụ ọrụ nhazi dị iche iche dị mkpa na sel.miRNA na-etinye aka n'ọtụtụ usoro ndụ nke na-ekpughe anụ ahụ - kpọmkwem na ogbo - okwu akọwapụtara yana echekwara nke ukwuu n'ụdị dị iche iche.
-
usoro nke circRNA-Ilumina
Emebere usoro transcriptome niile iji kọwaa ụdị mkpụrụ ndụ RNA niile, gụnyere koodu (mRNA) na RNA ndị na-abụghị koodu (gụnyere lncRNA, circRNA na miRNA) nke mkpụrụ ndụ akọwapụtara n'otu oge na-edegharị ya n'otu oge.Usoro transcriptome zuru ezu, nke a makwaara dị ka “nchịkọta RNA sequencing” na-achọ ikpughe netwọkụ usoro nhazi n'ọkwa transcriptome.N'iji teknụzụ NGS mee ihe, usoro nke ngwaahịa transcriptome niile dị maka nyocha ceRNA na nyocha RNA nkwonkwo, nke na-enye nzọụkwụ mbụ maka njirimara ọrụ.Na-ekpughe netwọk nchịkwa nke circRNA-miRNA-mRNA ceRNA dabere.
-
Usoro transcriptome niile - Illumina
Emebere usoro transcriptome niile iji kọwaa ụdị mkpụrụ ndụ RNA niile, gụnyere koodu (mRNA) na RNA ndị na-abụghị koodu (gụnyere lncRNA, circRNA na miRNA) nke mkpụrụ ndụ akọwapụtara n'otu oge na-edegharị ya n'otu oge.Usoro transcriptome zuru ezu, nke a makwaara dị ka “nchịkọta RNA sequencing” na-achọ ikpughe netwọkụ usoro nhazi n'ọkwa transcriptome.N'iji teknụzụ NGS mee ihe, usoro nke ngwaahịa transcriptome niile dị maka nyocha ceRNA na nyocha RNA nkwonkwo, nke na-enye nzọụkwụ mbụ maka njirimara ọrụ.Na-ekpughe netwọk nchịkwa nke circRNA-miRNA-mRNA ceRNA dabere.
-
Prokaryotic RNA usoro
Usoro prokaryotic RNA na-eji usoro ọgbọ na-abịa (NGS) kpughee ọnụnọ na ọnụọgụ RNA n'otu oge, site n'ịtụle transcriptome cellular na-agbanwe agbanwe.Usoro prokaryotic RNA nke ụlọ ọrụ anyị, ebumnuche kpọmkwem na prokaryotes nwere mkpụrụ ndụ ihe nketa, na-enye gị profaịlụ transcriptome, nyocha usoro mkpụrụ ndụ ihe nketa, wdg. etinyere ya n'ọtụtụ ebe na nyocha sayensị bụ isi, nyocha ọgwụ na mmepe, yana ndị ọzọ.
Platform: Illumina NovaSeq 6000
-
Usoro Metatranscriptome
Metatranscriptome sequencing na-achọpụta mkpụrụ ndụ ihe nketa nke ụmụ nje (ma eukaryotes na prokaryotes) n'ime gburugburu ebe obibi (ya bụ ala, mmiri, oke osimiri, nsị, na eriri afọ) .Kpọmkwem, ọrụ a na-enye gị ohere ịnweta nkọwapụta mkpụrụ ndụ dum nke obodo microbial dị mgbagwoju anya, nyocha taxonomic. ụdị dị iche iche, nyocha nkwalite ọrụ nke mkpụrụ ndụ ihe nketa dị iche iche, na ndị ọzọ.
Platform: Illumina NovaSeq 6000
