Usoro genome bisulfite dum
Uru ọrụ
● Ikpo okwu zuru ezu: nye otu nkwụsị ọrụ magburu onwe ya site na nhazi nlele, iwu ụlọ akwụkwọ, usoro na nyocha bioinformatics.
● Ezi izi ezi: Teknụzụ nhazi mgbanwe methylation tozuru oke nwere ike nyochaa ọnọdụ methylation nke otu isi C.
● Mkpuchi sara mbara: nchọpụta nke saịtị methylation na ọkwa genome-obosara.
● Ezi repeatability: kwụsiri ike nhazi, adabara ntụle ntụle n'etiti otutu samples.
Nkọwapụta sample
|
Platform
|
Gụọ Ogologo
|
Usoro usoro
|
| Illumina | PE 150 | 30X, ụdị mkpụrụ ndụ ihe nketa atọ, nwere genome ntụaka dị elu |
Ihe atụ chọrọ
|
Ụdị Nlereanya
|
Ọnụ ego (μg)
|
Conc.(ng/μl)
|
Olu (μl)
|
Ịdị ọcha
|
| gDNA | ≥ 2 | ≥ 20
| ≥ 20
| Mmebi na mmetọ nwere oke
|
Usoro ọrụ ọrụ
Nbufe ihe atụ
Mwepụta DNA
Ịrụ ụlọ akwụkwọ
Usoro
Nyocha data
Nnyefe data
Bioinformatics
1.Genome-obosara DNA methylation nkesa
2.Annotation na ukwuu-methylated CGI mpaghara
nweta ntinye okwu
Dee ozi gị ebe a ziga anyị ya
