Produk

Urutan DNA/RNA -PacBio Sequencer

Platform urutan PacBio minangka platform urutan sing wis suwe diwaca, sing uga dikenal minangka salah sawijining teknologi Urutan Generasi Katelu (TGS).Teknologi inti, siji-molekul wektu nyata (SMRT), nguatake generasi maca kanthi dawane puluhan kilo.Ing basis saka "Sequencing-by-Synthesis", resolusi nukleotida tunggal digayuh dening Zero-mode waveguide (ZMW), ing ngendi mung volume winates ing sisih ngisor (situs sintesis molekul), dipadhangi.Kajaba iku, urutan SMRT umume ngindhari bias spesifik urutan ing sistem NGS, amarga umume langkah amplifikasi PCR ora dibutuhake ing proses konstruksi perpustakaan.

Platform: Sekuel II, Revio

Hubungi Kita

Rincian Layanan

Asil Demo

Fitur

Rong mode urutan ing PacBio sequencer: Continuous Long Read (CLR) lan Circular Consensus Read (CCS)

Mode Sequencing

Ukuran Pustaka

Data TeoritisNgasilake (Per Sel)

Basis tunggalAkurasi

Aplikasi

CLR

20 Kb, 30 Kb, lsp.

80 Gb nganti 130 Gb

Kira-kira.85%

Saiki, SV nelpon, etc.

CCS

15-20 Kb

14 nganti 40 Gb/Sel (Sekuel II)

70 nganti 110 Gb/Sel (Revio)

Gumantung ing sampel

Kira-kira.99%

Saiki, Panggilan SNP/Indel/SV, Iso-Seq,

Perbandingan kinerja lan fitur Revio lan Sekuel II

Syarat	Sistem Sekuel II	Sistem Revo	Mundakake
Kapadhetan sing luwih dhuwur	8 yuta ZMWs	25 yuta ZMWs	3x
tataran independen	1	4	4x
Wektu mlaku luwih cendhek	30 jam	24 jam	1,25x
30X genom manungsa HiFi / taun	88	1.300	15x sakabèhé

Kaluwihan Service

● Swara 8 taun pengalaman ing platform urutan PacBio karo ewu proyek ditutup karo macem-macem spesies.

● Dilengkapi kanthi platform PacBio Sequencing paling anyar, Revio kanggo njamin throughput urutan sing cukup.

● Wektu giliran sing luwih cepet, ngasilake data sing luwih dhuwur lan data sing luwih akurat.

● Kontribusi ing atusan publikasi basis PacBio impact dhuwur.

Syarat Sampel

Tipe Sampel

Jumlah

Konsentrasi (Qubit®)

Volume

Kemurnian

liyane

DNA genomik

Gumantung ing Kebutuhan Data

≥50 ng/μl

≥15μl

OD260/280=1.7-2.2;
OD260/230 = 1.8-2.5;
Puncak bening ing 260 nm,ora ana kontaminasi

Konsentrasi kudu diukur kanthi Qubit lan Qubit/Nanopore = 0.8-2.5

RNA total

≥1,2 μg

≥120 ng/μl

≥15μl

OD260/280=1.7-2.5;
OD260/230=0.5-2.5;ora ana kontaminasi

Nilai RIN ≥7.5

5≥28S/18S≥1

Alur Kerja Layanan

Persiapan sampel

Konstruksi perpustakaan

Sequencing

Analisis data

Pangiriman proyek

Sadurunge: Produsen OEM Mrna Sequencing Service - Long non-coding sequencing-Illumina - Biomarker

Sabanjure: Illumina lan BGI

1. Hasil Data In-house

Data diasilake saka 63 sel CCS (saka 26 spesies)

DATA-PacBio-CCS-15 Kb	Rata-rata	Maks	Min	Median
Ngasilake - subreads (Gb)	421.12	544.27	221.38	426.58
Ngasilake - CCS(Gb)	25.93	38.59	10.86	25.43
Polimerase N50	145.651	175.430	118,118	144,689
Subwaca N50	17.509	23.924	12.485	17.584
CCS N50	14.490	19.034	9.876	14.747
Rata-rata dawa-Polymerase	67,995	89,379	49,664	66,433
Rata-rata dawa-Subreads	15.866	21.036	11.657	16.012
Rata-rata dawa-CCS	14.489	19.074	8.575	14.655

Data diasilake saka 16 sel CLR (saka 76 spesies)

DATA-PacBio-CLR-30Kb	Rata-rata	Maks	Min	Median
Ngasilake - subreads (Gb)	142.20	291.40	50.55	142.49
Polimerase N50	39.456	121.191	15.389	35.231
Subwaca N50	28.490	41.012	14.430	29.063
Rata-rata dawa-Polymerase	22.063	48,886	8.747	21.555
Rata-rata dawa-Subreads	17.720	27.225	8.293	17.779

2. Data QC – DemoStatistik babagan asil data

Sampel	ccs Wacan Nomer	Total basis ccs (bp)	ccs Wacan N50 (bp)	ccs Rata-rata Length (bp)	ccs Wacan Paling dawa (bp)	Subreads Bases (bp)	tingkat ccs (%)
PB_BMKxxx	3.444.159	54.164.122.586	15.728	15.726	36.110	863.326.330.465	6.27