16S/18S/EJUS Amplicon Sequencing-NGS
Servitium Commoda
Solitudo libero et celeri identificatio microbial compositionis in environmental samples
High resolution in low-abunda components in environmental samples
● Ultimae QIIME2 analyses fluunt cum diversis analysibus in terminis datorum, annotationis, OTU/ASV.
High-throughput, accuratius altius
Lorem diversis studiis communitatis microbial
● BMK amplam experientiam habet cum supra 100,000 exemplaria / anni tegimentum soli, aquae, gas, pituitae, faeces, intestina, cutis, fermentationis sorbitionis, insectorum, plantarum, etc.
● BMKCloud faciliorem reddit datam interpretationem continens 45 personales analyses instrumenta
Service Specifications
| SequencingPlatform | Bibliotheca | Commendatur data cede | Aestimari rursus, circa tempus |
| Illumina NovaSeq Platform | PE250 | 50K/100K/300K Tags | XXX diebus |
Bioinformatics analyses
Rudis notitia qualis imperium
OTU pampineis/De-strepitu(ASV)
● OTU annotation
Alpha diversitatem
Beta diversitatem
inter coetus analysis
● Analysis Association contra factores experimentales
Function gene vaticinium
Sample Requisita et Delivery
Sample Requisita:
ForDNA excerpta:
| Sample Type | Moles | Coniunctis | Puritas |
| DNA excerpta | 30 ng | 1 ng/µl | OD260/280= 1.6-2.5 |
Ad exempla environmental:
| Sample type | Commendatur sampling procedure |
| Solum | Sampling amount: approx.5 g;Manens autem substantia arida debet a superficie removeri;Tere magna frusta et per 2 mm sparguntur;Aliquot specimina in sterili EP-tube vel cyrotube ad reservationem. |
| feces | Sampling amount: approx.5 g;Collecta et aliquot exempla in EP-tube vel cryotube sterili ad reservationem. |
| Intestina contenta | Exempla necesse est sub conditione aseptic discursum.Lava collecta cum PBS;Centrifuge PBS et praecipitantem in EP-tubes collige. |
| Sludge | Sampling amount: approx.5 g;Collecta et aliquot pituitae specimen in sterili ep-tube vel cryotube ad reservationem |
| Waterbody | Specimen pro limitata copia microbialium, ut sonum aquae, putei aquae, etc., collige saltem 1 L aquam et per 0.22 µm colum transi, ut microbialem in membrana locupletet.Membranam in tubo sterili repone. |
| Cutis | Superficiem cutem diligenter corradunt cum swab bombicis sterilibus vel chirurgicis mucrone et in tubo sterili pone. |
Sample Delivery commendatur
Exempla in nitrogenio liquido per 3—4 horas durata et in liquido nitrogenio vel -80 gradu ad longi temporis reservationem reponunt.Sample naviculas cum sicco-glacie requiritur.
Service Opus O
Sample traditio
Library construction
Sequencing
Analysis
Post-venditionis officia
1.Species distribution
2.Heat map
3.Rare factionis curvae
4.NMDS analysis
5.Lefse analysis
BMK Case
Individua obesa cum et sine Type 2 diabete diversam ventrem microbialem facultatem et compositionem functionis ostendunt
Published:Cell Host & Microbe, 2019
De consilio sequendo:
Lean non-diabetes (n=633);Obesae non-diabetae (n=494);Obese-Type 2 diabete (n=153);
Regio target: 16S rDNA V1-V2
Platform: Illumina Miseq (NGS-based amplicon sequencing)
Subsetae DNA excerpta metagenomica sequen- tio in Illumina Hisq subiecta sunt
Eventus key
Microbiales profilings morborum metabolicorum horum feliciter distinguuntur.
Comparando lineamenta microbiales ab 16S sequendo generatas, obesitas inventa est cum mutationibus in compositione microbiali, singularum notarum, praesertim diminutionis significantium in Akkermansia, Faecalibacterium, Oscillibacter, Alistipes, etc. Addendo, T2D inventa est cum incremento in Escherichia/shigella. .
Reference
Thingholm, LB, et al."Obesus Individua cum et sine Typus II diabete Monstrare diversae Viscera Microbial muneris capacitatem et compositionem."Cellula Hostiae & Microbe26.2(2019).
