DNA/RNA Sequencing - Nanopore Sequencer
Service Details Features
| Platform | Library Size | Theoretical Data Cedite (Per Cell) | Unius basis Accuracy | Applications |
| Nanopore | 8Kb, 10kb, 20kb, Ultralong, cDNA-PCR | 70-90Gb/Cell | 85-92% | SV vocatio, De novo, plena longitudo sequendi, Iso-Seq, Gene annotation, Detectio methylationis DNA. |
Servitium Commoda
● Plus 5 annorum experientia in PacBio sequens suggestum cum millibus inceptis clausis cum variis speciebus.
● BMKGENE socius officialis Nanopore Oxoniensis est, cum duplici suggestu RNA/DNA certificatione.
● Exemplaria amet sequentiarum cum apparatu perfecto et sufficienti sequela throughput.
● Ex suggestu Nanopore, plus quam 10 animal et planta Denovo investigationes in diariis celeberrimis internationalibus divulgatae sunt.
Sample Requisita
| Sample Type | Moles | Concentrtion (Qubit ®) | Magnitudo | Puritas | alii |
| Genomic DNA | Pendent Data opus est | ≥20ng/μl | ≥15μl | OD260/280=1.7-2.2; OD260/230≥1.5； Patet apicem 260 nm，no contaminationibus | Intentio metiri debet ab Qubit et Qubit/Nanopore ≤ 2 |
| Total RNA | ≥1.2μg | ≥100μg/μl | ≥15μl | OD260/280=1.7-2.5; OD260/230=0.5-2.5；no contaminationes | RIN valor ≥7.5 |
Service Workflow
Sample praeparatio
Library construction
Sequencing
Analysis
Project traditio
Data Quality aestimatione DNA Sample
Table 1. Statistics on clean data.
| BMKID | rawSeqNum | rawSumBase | cleanSeqNum | cleanSumBase | cleanN50Len | cleanN90Len | cleanMeanLen | cleanMaxLen | cleanMeanQual |
| DNA_BMK01 | 1,218,239 | 26.37 | 1,121,753 | 25.90 | 28,014 | 15,764 | 23,090 | 143,181 | 9 |
Data Quality aestimatione RNA Sample
Table 1. Statistics on clean data.
| File nomen | ClientID | ReadNum | BaseNum | N50 | MeanLength | MaxLength | MeanQscore |
| RNA_BMK001 | C2 | 8,947,708 | 4,047,236,840 | 398 | 452 | 129,227 | Q12 |
Figure 1. Read longitudinem distribution
Figure 2. Quality score distribution of clean data
Figure 3. Longitudo et qualitas score distributio mundi data
