Prokaryotic RNA sequencing

Prokaryoticus RNA sequens posterius utitur generationis sequence (NGS) ut praesentiam et quantitatem RNA dato momento patefaciat, analysi transumptom cellulosum mutatum.Societas nostra prokaryotica RNA sequencing, speciatim prokaryotes cum genomes referentibus intendit, tibi praebens transcriptum profilingum, structuram analysin gene, etc. Late applicatum est ad investigationes praecipuas scientiarum, inquisitionum medicamentorum et progressuum, et plura.

Platform: Illumina NovaSeq Platform

Nobis loquere

Service Details

Service Specifications

Rostra: Illumina NovaSeq suggestum, PE150

Bibliotheca type: 350bp inserta bibliotheca cDNA (pendet in apicem distributionis)

Sequentia belli: Paired-End 150 bp

Suadeo data copia: II Gb rudis notitia / sample

Bioinformatics

(1) Sequentia notitia qualitatis imperium: distributio nucleotidis, Base qualitas distributionis, rRNA ratio census;

(2) Sequentia alignment analysis: Alignment result statistics, Saturatio sequendi, Coverage sequendi;

(3) Relatio genome annotationis (NR, Helvetii-Prot, Pfam, COG, GO, KEGG);

(4) Analysis elocutio: elocutionis distributio (cum duobus vel pluribus exemplis), Analysis locutionis inter exempla (Analysis Venn, Correlation analysis, PCA analysis);

(5) Analysis differentialis expressionis (cum duobus vel pluribus exemplis);

(6) Gene analysis constituit: Venn analysis, analysis Cluster, Function analysis annotationis (COG, GO, KEGG), Function ditior analysis (GO, KEGG), Functio locupletatio graphi chordalis, Ipath analysis;

(7) sRNA analysis: sRNA praedictio, sRNA annotation (Blast, sRNAMap, sRNATarBase, SIPHT, Rfam), sRNA secundae compages praesagium, sRNA scopus gene praedictio;

(VIII) Analysis structurae transumptae: Operon analysis, transcriptio initium ac finis situs praedictionis, UTR annotationem et analysis, promotor series praedicationis, SNP/InDel analysis (SNP/InDel annotationem, SNP/InDel distributio regionalis, SNP statistics).