Repraesentatio Bisulfite Reducitur Sequencing (RRBS)
Service Specifications
Rostra: NovaSeq suggestum, PE150
Bibliotheca type: 200-400bp inserta bisulfite tractata DNA bibliotheca
Sequentia belli: Paired-End 150 bp
Suadeo data output: X Gb rudis notitia / sample
Sample Requisita
DNA quantitas: ≥ 2ug
DNA concentration: ≥ 20ng/μl.
Puritas: OD260/280 = 1.8 ad 2.0 sine deformitate vel RNA contagione
Bioinformatics analysis
Service Opus O
Sample traditio
Library construction
Sequencing
Analysis
Post-venditionis officia
a.Genome alignment Reference Statistics
Statistics mapping legit
Statistics of sequencing profunditatem et coverage de C site
Aexaggeratus coverage
Digestion efficientiam cuiusque sample
b.Methylation situs deprehensio
Index graduum methylationis in C situs
BS conversio
Methylated cytosine
Methylation gradu distributionis
c.Methylation map
Genome-wide
Genome elementum
d.Comparatio methylationis gradus inter exempla
Pairwise ratione CG methylation
PCA-CG methylation
Cillustrat CG methylation
d.Aliter methylationem analysis
Statistics de DMR
DMR longitudinem distribution
Hmanducare tabula
DMR distributio in targeted regione
