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Totum genome bisulte sequencing

DNA methylatio in quinta positione cytosine (5-mC) fundamentalem vim habet in expressione gene et actione cellularum.Abnormes methylationis exemplaria pluribus conditionibus et morbis consociata sunt, ut cancer.WGBS vexillum auri factum est ad methylationem genome dilatatam in una basi resolutionis investigandae.

Platform: Illumina NovaSeq Platform


Service Details

Bioinformatics

Demo Results

Servitium Commoda

● Perfice suggestum: praebent unum-statur egregium ministerium ab sample processui, constructione bibliothecae, sequelam ad analysin bioinformaticas.

● Maximum accurate: Methylation conversionis maturae processus technologiae potest accurate resolvere statum methylationis unius basis C.

● Lata coverage: detectio methylationis situs in plano genome-lato.

● Bonum repeatability: processui stabilis, comparativa analysi apta inter plura exempla.

 

Sample Specifications

 

Platform

 

 

Read Longitudo

 

 

Sequens Strategy

 

Illumina

PE 150

30X, tres replicat biologicas cum qualitate maxima referentia genome

Sample Requisita

 

Sample Type

 

 

Moles (μg)

 

 

Conc.(ng/μl)

 

 

Volumen (μl)

 

 

Puritas

 

gDNA

≥ 2

≥ 20

 

≥ 20

 

Limited degradatio et contagione

 

 

 

Service Opus O

Sample partus

Sample traditio

Gubernator experimentum

DNA extractionem

Bibliotheca Praeparatio

Library construction

Sequencing

Sequencing

Analysis

Analysis

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Data traditio


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  •  Bioinformatics

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    1.Genome-wide DNA methylation distribution

    3(1)

    2.Annotation in altus-mythylated regione CGI

    4(1)

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