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Low MOQ for Bacterial Genome - Non-Reference based mRNA sequencing – Illumina – Biomarker

Service Details

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abide by the contract", conforms on the market requirement, joins from the market competition by its good quality likewise as provides more comprehensive and superb support for customers to let them become large winner. The pursue of the company, is definitely the clients' pleasure for Transcriptome Sequencing Provider , Single Cell Sequencing Provider , Microsatellite , We wish to just take this opportunity to ascertain long-term enterprise relationships with clients from all over the earth.

Low MOQ for Bacterial Genome - Non-Reference based mRNA sequencing – Illumina – Biomarker Detail:

Features

Service Advantages

Sample Requirements and Delivery

Sample Requirements:

Nucleotides:

Purity	Integrity	Contamination	Amount
OD260/280≥1.7-2.5； OD260/230≥0.5-2.5；	For plants: RIN≥6.5; For animals: RIN≥7; 28S/18S≥1.0; limited or no baseline elevation	Limited or no protein or DNA contamination shown on gel.	Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg

Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×10⁷
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×10⁵, flash frozen in liquid nitrogen is recommended.

Blood samples:
PA×geneBloodRNATube;
6mLTRIzol and 2mL blood(TRIzol:Blood=3:1)

Recommended Sample Delivery

Container:
2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment:
1.Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2.RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services

Product detail pictures:

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Basic Bioinformatics

Competing Endogenous Rna

Magnetic Bead Dna Extraction

Mrna Analysis

Spatial Single Cell Rna Seq

Transcriptome Analysis Methods

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