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Manufactur standard Metabarcoding Service - Eukaryotic mRNA sequencing-Illumina – Biomarker

Service Details

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We have now sophisticated machines. Our solutions are exported to the USA, the UK and so on, enjoying a great reputation amid consumers for Nanopore Metagenomics , Shotgun Sequencing Cost , Sequencing Cost , As a leading manufacture and exporter, we appreciate a great status inside the international markets, especially in America and Europe, because of our top high-quality and sensible charges.

Manufactur standard Metabarcoding Service - Eukaryotic mRNA sequencing-Illumina – Biomarker Detail:

Advantages

Sample Requirements and Delivery

Library	Sequencing strategy	Data recommended	Quality Control
Poly A enriched	Illumina PE150	6 Gb	Q30≥85%

Sample Requirements:

Nucleotides:

Purity	Integrity	Amount
OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel.	For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation	Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg

Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×10⁶ - 1×10⁷
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.

Blood samples: Volume≥1 ml

Microorganism: Mass ≥ 1 g

Recommended Sample Delivery

Container: 2 ml centrifuge tube (Tin foil is not recommended)

Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment:

Dry-ice: Samples need to be packed in bags and buried in dry-ice.
RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services

Product detail pictures:

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The key to our success is "Good Products Good quality, Reasonable Value and Efficient Service" for Manufactur standard Metabarcoding Service - Eukaryotic mRNA sequencing-Illumina – Biomarker , The product will supply to all over the world, such as: Paraguay , Guatemala , Marseille , Our Company has professional engineers and technical staff to answer your questions about maintenance problems, some common failure. Our product quality assurance, price concessions, any questions about the products, Please feel free to contact us.

Circrna Database