Manufactur standard Metabarcoding Service - Nanopore-based metagenome sequencing – Biomarker
Manufactur standard Metabarcoding Service - Nanopore-based metagenome sequencing – Biomarker Detail:
Service Advantages
Service Specifications
Bioinformatics analyses
ü Raw data quality control
ü Metagenome assembly
ü Non-redundant gene set and annotation
ü Species diversity analysis
ü Genetic function diversity analysis
ü Inter-group analysis
ü Association analysis against experimental factors
Sample Requirements and Delivery
Sample requirements and delivery
Sample Requirements:
For DNA extracts:
|
Sample Type |
Amount |
Concentration |
Purity |
|
DNA extracts |
＞ 30 ng |
＞ 1 ng/μl |
OD260/280= 1.6-2.5 |
For environmental samples:
|
Sample type |
Recommended sampling procedure |
|
Soil |
Sampling amount: approx. 5 g; Remaining withered substance needs to be removed from surface; Grind large pieces and pass through 2 mm filter; Aliquot samples in sterile EP-tube or cyrotube for reservation. |
|
Feces |
Sampling amount: approx. 5 g; Collect and aliquot samples in sterile EP-tube or cryotube for reservation. |
|
Intestinal contents |
Samples need to be processed under aseptic condition. Wash collected tissue with PBS; Centrifuge the PBS and collect the precipitant in EP-tubes. |
|
Sludge |
Sampling amount: approx. 5 g; Collect and aliquot sludge sample in sterile EP-tube or cryotube for reservation |
|
Waterbody |
For sample with limited amount of microbial, such as tap water, well water, etc., Collect at least 1 L water and pass through 0.22 μm filter to enrich microbial on the membrane. Store the membrane in sterile tube. |
|
Skin |
Carefully scrape skin surface with sterile cotton swab or surgical blade and place it in sterile tube. |
Recommended Sample Delivery
Freeze the samples in liquid nitrogen for 3-4 hours and store in liquid nitrogen or -80 degree to long-term reservation. Sample shipping with dry-ice is required.
Service Work Flow
Sample delivery
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
Our firm aims to operating faithfully, serving to all of our consumers , and working in new technology and new machine continually for Manufactur standard Metabarcoding Service - Nanopore-based metagenome sequencing – Biomarker , The product will supply to all over the world, such as: Costa Rica , Miami , Palestine , We pay high attention to customer service, and cherish every customer. We've maintained a strong reputation in the industry for many years. We are honest and work on building a long-term relationship with our customers.
By Marjorie
from Czech Republic
- 2018.12.10 19:03
The company can keep up with the changes in this industry market, product updates fast and the price is cheap, this is our second cooperation, it's good.
By Elsie
from Palestine
- 2017.09.30 16:36
get a quote
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