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Manufacturer for Structure Variation -
De novo Full-length Transcriptome sequencing -PacBio – Biomarker Detail:
Service Advantages
Service Specifications
ü Raw data processing
ü Transcript identification
ü Sequence structure
ü Expression Quantification
ü Function Annotation

Sample Requirements and Delivery
Sample Requirements:
Nucleotides:
| Amount |
Total ≥ 3 μg; Conc. ≥ 300 ng/μl; Volumn ≥ 10 μl |
| Integrity |
For plants: RIN ≥ 7.5; For animals: RIN ≥ 8; 28S/18S ≥ 1.0; Limited or no baseline elevation |
| Purity |
OD260/280 ≥ 1.8; OD260/230 ≥ 1.0; Clear peak at 260 nm |
| Note |
Protein or DNA contamination is very likely to affect the measurements and the samples’ QC level. |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 – 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Microorganism: Mass ≥ 1 g
Recommended Sample Delivery
Container:
2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2.RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow

Experiment design

Sample delivery

RNA extraction

Library construction

Sequencing

Data analysis

After-sale services
Product detail pictures:
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The organization upholds the philosophy of "Be No.1 in good quality, be rooted on credit history and trustworthiness for growth", will keep on to provide previous and new customers from home and overseas whole-heatedly for
Manufacturer for Structure Variation -
De novo Full-length Transcriptome sequencing -PacBio – Biomarker , The product will supply to all over the world, such as: Luxemburg
, Algeria
, Bahamas
, Since always, we adhering to the "open and fair, share to get, the pursuit of excellence, and creation of value"values, adhere to the"integrity and efficient, trade-oriented, best way , best valve" business philosophy. Together with our all over the world have branches and partners to develop new business areas, maximum common values. We sincerely welcome and together we share in global resources, opening up new career together with the chapter.