Te Raupapa Metatranscriptome
Ngā Āhuatanga Ratonga
● te whakaiti i te rRNA, ā, ka whai muri ko te whakarite i te whare pukapuka mRNA aronga.
● Te raupapatanga i runga i te Illumina NovaSeq.
Ngā Painga Ratonga
●Akohia ngā Huringa o ngā Hapori Moroiti Matatini:Ka puta tēnei i te taumata whakawhitiwhiti kōrero, ā, ka tūhuratia ngā ira hou pea.
●Te Whakamārama i ngā Taunekeneke o te Hapori Moroiti ki te Manaaki, ki te Taiao rānei.
●Tātaritanga Pūtaiao Koiora WhānuiMā tēnei ka kitea ngā mōhiotanga mō ngā hanganga whakarōpūtanga me ngā mahi a te hapori, tae atu ki te tātaritanga rerekētanga o te whakaaturanga ira.
●He Whakarāpopototanga Ira Whānui:Te whakamahi i ngā pātengi raraunga mahi ira hou mō ngā mōhiohio whai kiko mō te whakaaturanga ira o ngā hapori moroiti.
●Tautoko Whai muri i te Hokonga:E toru marama te roa o tā mātou whakapau kaha ki te whakaoti i te kaupapa, ā, ka tukuna atu he ratonga whai muri i te hoko mō ngā marama e toru. I tēnei wā, ka tukuna atu e mātou he āwhina ki te rapu raruraru, me ngā wā pātai me ngā whakautu hei whakautu i ngā pātai e pā ana ki ngā hua.
Ngā Whakatakotoranga Ratonga
| Pūhara raupapatanga | Rautaki Raupapa | Ngā raraunga e taunakitia ana | Mana Whakahaere Kounga Raraunga |
| Illumina NovaSeq | PE150 | 12Gb | Q30≥85% |
Ngā Whakaritenga Tauira
| Te kukū (ng/µL) | Te tapeke o te moni (µg) | Rōrahi (µL) | OD260/280 | OD260/230 | RIN |
| ≥50 | ≥1.0 | ≥20 | 1.8-2.0 | 1.0-2.5 | ≥6.5 |
Rere Mahi Ratonga
Tukunga tauira
Te hanganga whare pukapuka
Te raupapatanga
Tātari raraunga
Ngā ratonga i muri i te hoko
Kei roto ko te tātaritanga e whai ake nei:
● Te Whakahaere Kounga Raraunga Raupapa
● Huinga Tuhinga
● He Whakarāpopototanga Whakarōpū me te Rahinga
● He Tuhipoka Mahi me te Rahinga
● Te Ine Whakapuakitanga me te Tātari Rerekētanga
Te tohatoha whakarōpū o ia tauira:
Tātaritanga kanorau beta: UPGMA
Tuhipoka mahi - te nui o te GO
Te nui o te whakarōpūtanga rerekētanga – LEFSE
Tūhurahia ngā whanaketanga i āwhinahia e ngā ratonga raupapatanga meta-tātaritanga a BMKGene mā roto i tētahi kohinga pukapuka kua whiriwhiria.
Lu, Z. et al. (2023) 'Ko te ātete waikawa o ngā huakita whakamahi lactate o te raupapa Bacteroidales ka whai wāhi ki te aukati i te waikawa ruminal i roto i ngā koati kua taunga ki te kai kukū-nui',Kai Kararehe, 14, api 130–140. doi: 10.1016/J.ANINU.2023.05.006.
Song, Z. et al. (2017) 'Te wetewete i ngā moroiti mahi matua i roto i te whakapūtanga totoka tuku iho mā te whakamahi i ngā amplicons tere-rahi me te raupapatanga metatranscriptomics',Ngā Rohe i roto i te Microbiology, 8(HŌNGONGO). doi: 10.3389/FMICB.2017.01294/FULL.
Wang, W. et al. (2022) 'Ngā Mycovirus Hou i Kitea mai i te Rangahau Metatranscriptomics o te Harore Alternaria Phytopathogenic',Ngā huaketo, 14(11), whārangi 2552. doi: 10.3390/V14112552/S1.
Wei, J. et al. (2022) 'E whakaatu ana te tātari metatranscriptome whakarara i te whakahekenga o ngā matū tuarua o te tipu e ngā pītara me ō rātou hoa puku',Ngā ngota Te Taiao, 31(15), wh.3999–4016. doi: 10.1111/MEC.16557.
