mRNA-seq (NGS) – Nō nā tata nei

Mā te raupapatanga mRNA ka taea te whakaahua i ngā tuhinga mRNA katoa i roto i ngā pūtau i raro i ngā āhuatanga motuhake, ā, he hangarau whānuitia tēnei e whakamahia ana i roto i ngā momo mara rangahau. Kua hangaia te paipa raupapatanga mRNA-de novo BMKCloud hei tātari i ngā whare pukapuka raupapatanga whai rawa poly-A ina kore he ira tohutoro e wātea ana. Ka tīmata te paipa me te whakahaere kounga, ā, ka whai ake konō nā tata neite huihuinga tuhinga me te kōwhiringa huinga ira kotahi. Ka matapae te tātari hanganga ira kotahi i te raupapatanga waehere (CDS) me ngā tukurua raupapatanga māmā (SSR). Muri iho, ka kitea e te tātari whakapuakanga rerekētanga ngā ira kua whakapuakanga rerekētanga (DEG) i roto i ngā āhuatanga kua whakamatautauria, ā, ka whai ake ko te tuhipoka mahi me te whakarei ake i ngā DEG hei tango i ngā māramatanga koiora.