Pacbio Te tuhinga roa-katoa

Mā te PacBio Full-Length Transcriptome Sequencing, Isoseq, ka taea te tautuhi tika i ngā momo tuhinga, e whakamārama ana i te polyadenylation me te splicing rerekē, ā, ka ārahi ki te tātaritanga whakaaturanga ira tika ake. Kua hangaia te paipa BMKCloud PacBio Full-Length Transcriptome hei tātari i ngā whare pukapuka cDNA kua raupapahia i roto i te aratau Circular Consensus Sequencing (CCS) me te tautuhi i ngā raupapatanga Full-Length Non-Chimeric (FLNC), ka whakarōpūhia ki roto i ngā tuhinga kore-taupoki. Ka aromatawaihia e te tātaritanga BUSCO i muri mai te otinga o te huihuinga transcriptome. Mai i te transcriptome kua huihuia, ka mahia ngā tātaritanga maha: splicing rerekē, te tukurua raupapatanga māmā (SSR), te matapae o te lncRNA me ngā ira ūnga e rite ana, te matapae o ngā ira hou, te tātaritanga whānau ira, te tātaritanga ira whakawhitiwhiti, me te tuhipoka mahi o ngā tuhinga.