Te raupapa RNA prokaryotic

Whakaraupapa RNA Prokaryotic Whakaahua Whakaatuhia

Ko te raupapa RNA Prokaryotic e whakamahi ana i te raupapa reanga o muri mai (NGS) ki te whakaatu i te aroaro me te nui o te RNA i tetahi wa, ma te tātari i te hurihanga o te whakawhitinga waea.Ko te raupapa RNA prokaryotic a to taatau kamupene, e whai ana ki nga prokaryotes me nga tohu tohutoro, e whakarato ana ki a koe he tuhinga tuhi, tātari hanganga ira, me etahi atu.

Paerewa: Illumina NovaSeq Platform

Whakapā mai

Nga Taipitopito Ratonga

Whakatakotoranga Ratonga

Paerewa: Illumina NovaSeq turanga, PE150

Momo whare pukapuka: 350bp te whakauru i te puna cDNA (e whakawhirinaki ana ki te tohatoha tihi)

Rautaki Whakaraupapa: Takirua-Whakamutunga 150 bp

Tautohu te nui o te raraunga: 2 Gb raw raraunga/tauira

Bioinformatics

(1) Te raupapa o te mana o te kounga raraunga: Te tohatoha nucleotide, te tohatoha kounga turanga, te aromatawai o te rRNA;

(2) Te wetewete i te raupapatanga o te raupapa: Nga tatauranga o nga hua o te whakatikatika, Te Whakanoho o te raupapa, Te kapi o te raupapa;

(3) Tohutoro tohu tohu ira (NR, Swiss-Prot, Pfam, COG, GO, KEGG);

(4) Te wetewete korero: Te tohatoha korero (Me nga tauira e rua, neke atu ranei), Te tātaritanga o te whakapuaki i waenga i nga tauira (Te wetewete Venn, Te whakamaarama hononga, te tātari PCA);

(5) Te tātari korero rerekee (Me nga tauira e rua, neke atu ranei);

(6) Te tātari huinga ira: Te tātari Venn, Te tātari Cluster, Te tātari tohu mahi (COG, GO, KEGG), Te tātaritanga whakarangatira Mahi (GO, KEGG), Te kauwhata whakarangatira mahi, te tātari Ipath;

(7) sRNA tātaritanga: sRNA matapae, sRNA annotation (Blast, sRNAMap, sRNATarBase, SIPHT, Rfam), sRNA matapae hanganga tuarua, sRNA matapae ira ira;

(8) Te tātaritanga hanganga tuhinga: Te tātari Operon, Te tiimata me te mutunga o nga waahi matapae, te tohu UTR me te tātaritanga, te matapae raupapa kaiwhakatairanga, te tātari SNP/InDel (SNP/InDel annotation, SNP/InDel tohatoha rohe, SNP tatauranga).