Whakaitihia te Whakaaturanga Bisulfite Sequencing (RRBS)
Whakatakotoranga Ratonga
Paerewa: NovaSeq turanga, PE150
Momo whare pukapuka: 200-400bp te whakauru i te whare pukapuka DNA kua tukinotia te bisulfite
Rautaki Whakaraupapa: Takirua-Whakamutunga 150 bp
Tutohu putanga raraunga: 10 Gb raw raraunga/tauira
Nga Whakaritenga Tauira
Te rahinga DNA: ≥ 2ug
Te kukū DNA: ≥ 20ng/μl.
Maamaa: OD260/280 = 1.8 ki te 2.0 kaore he paheketanga, he poke RNA ranei
Te tātari bioinformatics
Te Rerenga Mahi Ratonga
Te tuku tauira
Te hanga whare pukapuka
Whakaraupapa
Te tātari raraunga
Nga ratonga muri-hoko
a.Tohutoro tatauranga tirohanga ira ira
Nga tatauranga o nga panui mapi
Snga tatauranga mo te hohonu o te raupapa me te kapi o te waahi C
Akapi kua whakaemihia
Dte whai huatanga o ia tauira
b.Te kimi pae methylation
Te rarangi o nga taumata methylation i te waahi C
BS hurihanga
Mcytosine ethylated
Te tohatoha taumata methylation
c.Mapi methylation
Gtaitamariki-whanui
Ghuānga enome
d.Te whakataurite i nga taumata methylation i waenga i nga tauira
PAirwise Te hononga o te CG methylation
PCA-CG methylation
Cte whakamarama o te CG methylation
d.Te tātari methylation rereke
SKo nga waahanga mo te DMR
Te tohatoha roa DMR
Hkai mapi
DTe tohatoha MR ki te rohe kua tohua
