Te Raupapa RNA Iti-Illumina
Ngā Āhuatanga
● Te whakarite i te kōwhiringa rahi o te whare pukapuka.
● Ko te tātaritanga koiora-pūtaiao i aro ki te matapae me ngā whāinga o te miRNA.
Ngā Painga Ratonga
●Tohungatanga WhānuiNeke atu i te 300 ngā tauira kua tukatukahia e mātou, tae atu ki ngā momo tauira rerekē. Ka kawea mai e mātou te nui o ngā pūkenga ki ia kaupapa.
●Mana Kounga PūmauKa whakatinanahia e mātou ngā pūwāhi whakahaere matua puta noa i ngā wāhanga katoa, mai i te whakarite tauira ki te whakarite whare pukapuka, te raupapatanga me te hangarau koiora. Mā tā mātou aroturukitanga tino tupato ka puta he hua kounga teitei tonu.
●Tātaritanga whānui mō te koiora-pārongoā:Mā tēnei ka taea te tautuhi i ngā miRNA e mōhiotia ana me ngā miRNA hou, te tautuhi i ngā whāinga miRNA, me te tuhipoka mahi e rite ana me te whakarei ake mā te whakamahi i ngā pātengi raraunga maha (KEGG, GO).
●Tautoko Whai muri i te HokongaE mārama ana mātou ki te hiranga o te noho mai, koia mātou i whakapau kaha ai ki tua atu i te otinga o te kaupapa me te wā ratonga i muri i te hoko e toru marama te roa. I tēnei wā, ka tukuna e mātou he whai i te kaupapa, he āwhina rapurongoā, me ngā wā pātai me ngā whakautu hei whakautu i ngā pātai e pā ana ki ngā hua.
Ngā Whakaritenga Tauira me te Tukunga
| Whare Pukapuka | Papaaho | Ngā raraunga e taunakitia ana | Te Whakatau Raraunga |
| Rahi kua tīpakohia | Illumina SE50 | 10M-20M ngā pānui | Q30≥85% |
Ngā Whakaritenga Tauira:
Ngā nukleotida:
| Te nui o te konupūmā (ng/μl) | Te nui (μg) | Te parakore | Ngākau pono |
| ≥ 80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 He iti noa iho, kāore rānei he poke o te pūmua, o te DNA rānei e kitea ana i runga i te tiēre. | RIN≥6.0; 5.0≥28S/18S≥1.0; te pikinga turanga iti, kāore rānei |
● Ngā tipu:
Pūtake, Kākano, Putiputi rānei: 450 mg
Rau, Kākano rānei: 300 mg
Hua: 1.2 karamu
● Kararehe:
Ngākau, te Puku rānei: 450 mg
Ngā whekau, te roro rānei: 240 mg
Uaua: 600 mg
Ngā wheua, ngā makawe, te kiri rānei: 1.5g
● Ngā Angaanga:
Ngā pepeke: 9g
Ngā Kōura: 450 mg
● Toto katoa: 2 ngā ngongo
● Ngā pūtau: 106 ngā pūtau
● Te toto me te toto toto:6 mL
Tukunga Tauira i Manakohia
Ipu: ngongo whakapūkara 2 ml (Kāore i te tūtohutia te pepa konumohe)
Tauira tapanga: Rōpū+tāruatia hei tauira A1, A2, A3; B1, B2, B3.
Tukunga:
1. Hukapapa maroke: Me tākai ngā tauira ki roto i ngā putea ka tanumia ki roto i te hukapapa maroke.
2. Ngā ngongo RNA pumau: Ka taea te whakamaroke i ngā tauira RNA i roto i te ngongo pumau RNA (hei tauira, RNAstable®) ka tukuna i te pāmahana rūma.
Rere Mahi Ratonga
Te hoahoa whakamātautau
Tukunga tauira
Te tango RNA
Te hanganga whare pukapuka
Te raupapatanga
Tātari raraunga
Ngā ratonga i muri i te hoko
Pūtaiao Koiora
● Te whakahaere kounga raraunga mata
● Te whakarōpūtanga RNA iti
● te whakapuakanga miRNA me te tātaritanga whakapuakanga rerekētanga
● ira ūnga miRNA me te tuhipoka ira ūnga miRNA rerekē te whakaaturanga
● Te tuhipoka ira ūnga me te whakarei ake i te mahi ira miRNA rerekē te whakaaturanga
Te tautuhi i te miRNA: te hanganga me te hohonutanga
Te rerekētanga o te whakaaturanga o te miRNA – te whakarōpūtanga hiearchal
Te whakamārama mahi o te ūnga o ngā miRNA rerekē te whakaaturanga
Tūhurahia ngā whanaketanga rangahau i āwhinahia e ngā ratonga raupapatanga sRNA a BMKGene mā roto i tētahi kohinga pukapuka kua whiriwhiria.
Chen, H. et al. (2023) 'Ka aukati ngā mate huaketo i te hanga saponin me te whakaahua i roto i te Panax notoginseng',Te Ahupūngao me te Matū Koiora o ngā Tipu, 203, whārangi 108038. doi: 10.1016/J.PLAPHY.2023.108038.
Li, H. et al. (2023) 'Ka hono te pūmua FREE1 kei roto i te rohe FYVE o te tipu ki ngā wāhanga tukatuka iti hei pehi i te hanga koiora miRNA',Ngā pūrongo a te EMBO, 24(1). doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yu, J. et al. (2023) 'Ko te MicroRNA Ame-Bantam-3p e Whakahaere ana i te Whanaketanga o te Pupa Larval mā te Whai i te Ira 8 o te Epidermal Growth Factor-like Domains (megf8) i roto i te Pī Honī, Apis mellifera',Te Hautaka ā-Ao mō ngā Pūtaiao Ngota, 24(6), whārangi 5726. doi: 10.3390/IJMS24065726/S1.
Zhang, M. et al. (2018) 'Ko te Tātaritanga Whakauru o te MiRNA me ngā Ira e Pā Ana ki te Kounga Mīti e Whakaatu ana ko te Gga-MiR-140-5p te Pānga ki te Putunga Ngako I Roto i ngā Uaua i roto i ngā Heihei',Te Pūtaiao Pūtau me te Matū Koiora, 46(6), api 2421–2433. doi: 10.1159/000489649.
