Te raupapatanga bisulfite genome katoa
Painga Ratonga
● Papai oti: whakarato ratonga pai kotahi-mutu mai i te tukatuka tauira, te hanga whare pukapuka, te raupapatanga ki te tātari bioinformatics.
● Te tino tika: Ka taea e te hangarau tukatuka whakawhiti methylation pakeke te tātari tika i te mana methylation o te turanga C kotahi.
● Kapi whanui: te kimi i nga waahi methylation i te taumata ira-whanui.
● He pai te tukurua: te tukatuka pumau, e tika ana mo te tātari whakatairite i waenga i nga tauira maha.
Tauira Tauira
|
Paerewa
|
Pānui Te Roa
|
Rautaki Raupapa
|
| Maramarama | PE 150 | 30X, e toru nga tauira koiora, me te tohutoro tohutoro teitei |
Nga Whakaritenga Tauira
|
Momo Tauira
|
Rahi (μg)
|
Conc.(ng/μl)
|
Rōrahi (μl)
|
Maamaa
|
| gDNA | ≥ 2 | ≥ 20
| ≥ 20
| He iti te paheketanga me te poke
|
Te Rerenga Mahi Ratonga
Te tuku tauira
tangohanga DNA
Te hanga whare pukapuka
Whakaraupapa
Te tātari raraunga
Te tuku raraunga
Bioinformatics
1.Genome-wide DNA methylation tohatoha
2.Annotation i runga i te rohe CGI tino-methylated
