Bmkcloud Lowani

Malo

16s / 18s / ampricon sequening-pacbio

16s / 18s / ampricon squenic-pacbio yowonetsedwa

A 16S ndi 18s RRNA majini, limodzi ndi dera lomwe adalemba malo (ake), ndikupanga zikwangwani zam'manja zomwe zimapangidwa ndi zikwangwani zokhala ndi zida zomwe zimapangidwa ndi prokaryotic. Kukula kwa madera amenewa kumapereka njira yodzisankhira njira yofufuzira ma microbial pofufuza zachilengedwe komanso zosiyanasiyana zachilengedwe. Pomwe zingwe zimazungulira zigawo zazifupi ngati V3-v4 ya 16s ndi ik1, zawonetsedwa kuti zodziwika bwino za msonkho wopambana ndizotheka kutsata kutalika kwa 16s kutalika kwa 16s, 18s, ndi zake. Njira zokwanirazi zimapangitsa kuti magawo azigawo omwe amawerengedwa molondola, akukwaniritsa gawo la chisankho zomwe zimafikira kwa mitundu ya mitundu. Pulogalamu yopanda pake ya paccuo (SMRT) yomwe ikuchitika mwa kuchuluka kwa mabatani athunthu (HIFI) omwe amawerengera kutalika kwake, akumamenya mawu a Audina. Uwu uwu umalola ofufuza kuti apeze mwayi wosasunthika - mawonekedwe a chibadwa cha mawonekedwe amtunduwu. Kupeza kowonjezereka kumakweza malingaliro amitundu yosiyanasiyana, makamaka mkati mwa mabakiteriya kapena m'magulu a mabakiteriya, kumathandizira kuzindikira mwakuya anthu ambiri.

Lumikizanani nafe

Zambiri

Bioinfoctic

Zotsatira za Demo

Zolemba zolembedwa

Mawonekedwe antchito

● Pulatifomu Yabwino: Pacbio Revio

● Njira yoyeserera: CCS (HIFI imawerengera)

● Kukwezedwa kwa Dera la Ngongole

Ubwino Wantchito

●Kusintha kwa Taxnononoma: tHan Shir-Amminricn,kuthandizira otalika otha otha pamtundu wa mitundu.

●Kuyitanitsa Kolondola Kwambiri: Pacbio ccs mode (Hifi amawerengedwa).

●Kudzipatula: Kuzindikira mwachangu kwa microbial kapangidwe mu chilengedwe.

●Zofunika Kwambiri: Maphunziro osiyanasiyana osiyanasiyana.

●Kusanthula Kwambiri bioin: Phukusi laposachedwa la qiime2 (chidziwitso chokwanira mu chitukuko chaching'ono) ndi kusanthula kosiyanasiyana malingana ndi database, kusuta, Otu / ASV.

●Waluso kwambiri: Ndi ma projekiti zikwizikwi osindikizira, BMKGene amabweretsa zaka khumi, gulu laukadaulo waluso, lofananira, komanso chithandizo chabwino kwambiri.

Zolemba

Nkhokwe ya mabuku	Njira Yolowetsa	Zambiri Zotsimikiziridwa
Apomphon	Pacbio Revio	10/30/50 k tags (CCS)

Zofunikira Zitsanzo

Kukhazikika (zg / μl)	Kuchuluka kwathunthu (μg)	Voliyumu (μl)
Bi	≥0.3	≥20

Kulimbikitsidwa Kwambiri

Yatsani zitsanzozo m'madzi a nayitrogeni kwa maola 3-4 ndikusunga mu madzi a nayitrogeni kapena -80 degree kuti isungidwe kwa nthawi yayitali. Kutumiza kwachitsanzo ndi ma ayezi oundana ndikofunikira.

Ntchito Yogwira Ntchito

Kutumiza kwachitsanzo

Ntchito Yomanga Laibulale

Kusazidza

Kusanthula kwa data

Ntchito Zogulitsa Pambuyo

M'mbuyomu: 16s / 18s / zingwe za ampricon-ng

Ena: Gawani Chromatin-In Chromatin yopezeka ndi zigawenga zazitali (ATAC-seq)

Mulinso kuwunika:

● Zolemba zapamwamba

● Otut Cluster / de-noise (ASV)

● OTU chibadwa

● Kusanthula kwa Afa 10: Indexesties, kuphatikiza Shannon, Simpson ndi Ace.

● Kusanthula kwa Beta

● Kusanthula kwa magulu

● Kusanthula kwa Contration: Pakati pazinthu zachilengedwe ndi kuphatikizika ndi kusiyanasiyana

● 16

Histogram yogawika taximoni

Mtengo wa Phylogenetic Mtengo

ALPHA KUSINTHA KWA ALLA: Ace

mapeto

Kusanthula kwa beta ku Beta: PCAA

Kusanthula kwa Intergroup: Aniva

Funsani zomwe zikugwirizana ndi BMKGEne's Ampicon Apquenicy Services ndi Pacbio kudzera pa zofalitsa zofalitsa.

Gao, X. ndi Wang, H. (2023) ' 1), p. 16. Doi: 2.3390 / Fermentation9010016 / S1.

Li, S. et al. . Doi: 10.1038 / S41522-043-00439-8.

Mu, L. et al. . 1487. Doi: 10.1002 / JSFA.11482.

Yang, J. et al. . Mu oxazolone-insung inniade opaleshoni yovuta kwambiri mu rabrayfish wamkulu ', antioxidants 2023, Vol. 12, tsamba 192, 12 (1), p. 192. Doi: 2.3390 / Antiox1201192.