De Novo bacteria caccome
Timapereka msonkhano wathunthu wonse, kutsimikizira 0 mipata. Izi ndizotheka polumikiza matekinoloje olewerera owerenga nthawi yayitali, monga Nanopore ndi Pacbio ya msonkhano komanso kuwerengera pang'ono ndi kuwunikira kutsimikizika kwa att. Ntchito yathu imapereka ndalama zonse za bioinmafolotic. Ntchitoyi imathandizira kukulitsa ma geniomi otchulidwa kwa maphunziro osiyanasiyana a majini. Kuphatikiza apo, imapanga maziko a ntchito zongokhalira ndi kukhalitsa kwaukadaulo, kukhazikitsa magwiridwe azamitundu, ndikuwonetsetsa kuti ndi zodalirika komanso zopanda nzeru.
Mawonekedwe antchito
● Ndi njira ziwiri zomwe zingathe kusankha kutengera kuchuluka kwa genome kukwanira.
● Njira yokonzekera matenda: Kuwerengera mwachidule ndi matenda a Novaseq Pe150.
● Malizitsani kutentha 0 gap.
● Zingwe zowerenga nthawi yayitali pa nanopore promethion 48 kapena pacbio Revio wa msonkhano.
● Kusachedwa kuwerengera pang'ono pa Evaiva Novaseq kwa genome kutsimikizika ndi kukonza cholakwika (nanopore) kapena kupanga genome yokonzekera.
Ubwino Wantchito
●Zero-gap genome yotsimikizika: Izi zimachitika chifukwa chophatikiza madioni osindikizira ndi mawonekedwe owerengera (nanopore kapena pacbio).
●Malizitsani Bioinformaticts Graeflow:Izi zimaphatikizapo msonkhano wamisonkhano komanso kuneneratu za zinthu zingapo za mitundu ya zojambulajambula, ntchito zogwira ntchito, komanso zowoneka bwino monga chiwembu chozungulira.
●Waluso kwambiri: Ndili ndi matenda oposa 20,000 omwe asonkhana, BMKgene amabweretsa zaka khumi, gulu laukadaulo waluso, lofananira, komanso chithandizo chabwino kwambiri.
●Thandizo la Kugulitsa:Kudzipereka kwathu kumapitilira kumaliza ntchito ndi mwezi wachitatu - nthawi yogulitsa ntchito. Panthawi imeneyi, timapereka thandizo lotsatira, kuvutitsa, ndi magawo kuti athetse mafunso okhudzana ndi zotsatira zake.
●Njira zambiri zotsatizana zomwe zilipo:Zolinga zosiyanasiyana za kafukufuku ndi zofunikira zamphamvu.
Zolemba
| Kutumikila | Njira Yolowetsa |
| Genfter genome | Audina Pe150 100X |
| 0 GoP Genome | Nanopore 100x + Audina Pe150 100X Or Pacbio HiFi 30x + EUPININ TO150 100X (Posankha) |
Zofunikira:
| Kukhazikika (zg / μl) | Kuchuluka kwathunthu (μg) | Voliyumu (μl) | Ode260 / 280 | Ode260 / 230 | |
| Pacbio | ≥20 | ≥1.2 | ≥20 | 1.7-2.2 | ≥1.0 |
| Nanomore | ≥40 | ≥2 | ≥20 | 1.7-2.2 | 1.0-3.0 |
| Idzima | ≥1 | ≥0.06 | ≥20 | - | - |
Mabacteria: ≥3.5x1010 malulowa
Ntchito Yogwira Ntchito
Kutumiza kwachitsanzo
Ntchito Yomanga Laibulale
Kusazidza
Kusanthula kwa data
Ntchito Zogulitsa Pambuyo
Mulinso kuwunika:
● Kusinthanitsa kwa data
● Msonkhano wa Gen
● Kusanthula kwa Genel Enterome: Kuneneratu za CDS ndi zinthu zingapo za mitundu
● Zogwiritsa ntchito zogwirira ntchito ndi ma database angapo (Pitani, Kegg, etc) ndi databases (khadi, vfdb, etc)
● Mawonekedwe a Genome
Timapereka mtundu womwe umapezeka mosavuta komanso faidating'ono (gff).
Rus Insung - Pitani
Rus Insumetion - Cazy Carbohhhydrates
Mtundu Watsopano - Mabwalo Ozungulira
Funsani zomwe zikugwirizana ndi BMKGEE BWINO BWINO BWINO BWINO
Dai, W. et al. (2023) 'Kupezeka kwa ma briteriaides kh18-22 ngati bactericium yotetezeka komanso yatsopano kwambiri yothandizira zilonda zam'mimba zokongoletsa zamphamvu ",Mabungwe apadziko lonse lapansi a sayansi, 24 (19), p. 14669. Doi: 10.3390 / ijms241914669 / S1.
Kang, Q. et al. .Zoology, 16 (6), pp. 798-809. Doi: 10,11111 / 17491-48.12510.
Wang, tt et al. .Journal of Biotechnology, 260, mas. 38-41. Doi: 10,1016 / J.Jbiotec.2017.09.001.
Wang, x. et al. (2021) 'Kafukufuku Wosakanikizana ndi ma plasmid a klebiella amakaniza kwambiri kufalitsa majini a antimicrobial',Madera aku Microbiology, 12, p. 754931. Doi: 10.3389 / Fmicb.2021.754939311 / BIBULO.
