Mabakiteriya ndi fungal Yense ya Genome Yotsatizana
Ntchito zotsatizananso za mabakiteriya ndi mafangasi amtundu wonse ndizofunikira kwambiri pakupititsa patsogolo ma genomics a microbial genomics pothandizira kukwaniritsidwa ndi kufananiza ma genome a microbial. Izi zimathandizira uinjiniya wa fermentation, kukhathamiritsa kwa njira zamafakitale, komanso kufufuza njira zachiwiri za metabolism. Kuphatikiza apo, kutsatizananso kwa mafangasi ndi mabakiteriya ndikofunikira kuti timvetsetse kusintha kwa chilengedwe, kukhathamiritsa zovuta, ndikuwulula zakusintha kwa majini, zomwe zimakhudza kwambiri zamankhwala, ulimi, ndi sayansi ya chilengedwe.
Ubwino wa Utumiki
●Kusanthula kwa Bioinformatic Kumaphatikizapo Kuyimba Kosiyanasiyana:Kupereka zidziwitso zogwira ntchito mu ma genome otsatizananso.
● Luso Lambiri: Ndi masauzande ambiri a mapulojekiti obwerezabwereza omwe amachitidwa chaka chilichonse, timabweretsa zaka zoposa khumi, gulu lofufuza laluso kwambiri, zonse zomwe zilipo, komanso chithandizo chabwino kwambiri cha pambuyo pogulitsa.
●Thandizo Pambuyo Pakugulitsa:Kudzipereka kwathu kumapitilira kutha kwa projekiti ndi nthawi ya miyezi itatu mutagulitsa. Panthawiyi, timapereka zotsatila za polojekiti, chithandizo chazovuta, ndi magawo a Q&A kuti tiyankhe mafunso aliwonse okhudzana ndi zotsatira.
Mafotokozedwe a Utumiki
| Sequencing nsanja | Njira Yotsatirira | Deta yovomerezeka | Kuwongolera khalidwe |
| Illumina NovaSeq | Mtengo wa PE150 | Kuzama kwa 100x | Q30≥85% |
Zitsanzo Zofunika
| Kukhazikika (ng/µL) | Ndalama zonse (ng) | Voliyumu (µL) |
| ≥1 | ≥60 | ≥20 |
Bakiteriya: ≥1x107 maselo
Bowa Unicellular: ≥5x106-1x107 maselo
Bowa lalikulu: ≥4g
Kuyenda kwa Ntchito Yantchito
Kupereka zitsanzo
Kumanga laibulale
Kutsata
Kusanthula deta
Pambuyo pogulitsa ntchito
Mulinso Kusanthula Kotere:
- Kutsata Kuwongolera Ubwino wa Data
- Kugwirizana kwa Reference Genome
- Kuyimba Kosiyanasiyana: SNP ndi InDel
- Mawu Osiyanasiyana
Kuyimba kosiyana: Mitundu ya SNP
Kuyimba kosiyana: Kugawa kwautali wa InDel
Onani kupita patsogolo komwe kumayendetsedwa ndi BMKGene's microbial genome re-saquencing services kudzera m'magulu osiyanasiyana.
Jia, Y. et al. (2023) 'Kuphatikizira Transcriptome ndi Genome Yonse Yotsatizananso ndi Matenda a Screen Resistance Genes for Wheat Dwarf Bunt',International Journal of Molecular Sciences, 24(24). doi: 10.3390/IJMS242417356.
Jiang, M. et al. (2023) 'Ampicillin-controlled glucose metabolism imapangitsa kusintha kuchoka ku kulolerana kupita kukana mabakiteriya',Kupita Patsogolo kwa Sayansi, 9(10). doi: 10.1126/SCIADV.ADE8582/SUPPL_FILE/SCIADV.ADE8582_SM.PDF.
Yang, M. et al. (2022) 'Aliidiomarina halalkaliphila sp. nov., bakiteriya ya haloalkaliphilic yomwe ili kutali ndi nyanja ya soda ku Inner Mongolia Autonomous Region, China', Int. J. Syst. Evol.Microbiol,72 p. 5263. doi: 10.1099/ijsem.0.005263.
Zhu, Z., Wu, R. ndi Wang, G.-H. (2024) 'Genome sequence of Staphylococcus nepalensis ZZ-2023a, kutali ndi Nasonia vitripennis',Zilengezo za Microbiology Resource. doi: 10.1128/MRA.00802-23.
