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PacBio-Full-length Transcriptome (Non-Reference)
Pulatifomu ya Amplicon (16S/18S/ITS) imapangidwa ndi zaka zambiri zakusanthula kwama projekiti osiyanasiyana, omwe amakhala ndi kusanthula koyambira ndi kusanthula kwamunthu payekha: kusanthula koyambira kumakhudza kusanthula kwakukulu kwa kafukufuku waposachedwa wa tizilombo tating'onoting'ono, zowunikira ndizolemera komanso zomveka, ndi zotsatira za kusanthula zimaperekedwa mu mawonekedwe a malipoti a polojekiti;Zomwe zili pakuwunika kwamunthu ndizosiyanasiyana.Zitsanzo zitha kusankhidwa ndipo magawo atha kukhazikitsidwa mosinthika malinga ndi lipoti lowunikira komanso cholinga cha kafukufuku, kuti mukwaniritse zomwe mukufuna.Mawindo opangira makina, osavuta komanso ofulumira.
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PacBio-Full-length Transcriptome (Non-Reference)
Kutengera data ya Pacific Bioscience (PacBio) Isoform yotsatirira monga zolowetsa, Pulogalamuyi imatha kuzindikira masanjidwe aatali athunthu (popanda kuphatikiza).Popanga mapu a mautali athunthu motsutsana ndi ma genome, zolembedwa zimatha kukongoletsedwa ndi majini odziwika, zolembedwa, zigawo zolembera, ndi zina zotere. Pankhaniyi, chizindikiritso cholondola cha mapangidwe a mRNA, monga kuphatikizika kwina, ndi zina, zitha kukwaniritsidwa.Kusanthula kophatikizana ndi data ya NGS transcriptome sequencing kumathandizira kutanthauzira momveka bwino komanso kuchulukitsidwa kolondola kwambiri pofotokozera pamlingo wa zolembera, zomwe zimapindulitsa kwambiri kusiyanasiyana kwamitundu ndi kusanthula kwamachitidwe.
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Zida Zazida
BMKCloud ndi nsanja yotsogola ya bioinformatic yopereka njira imodzi yokha yamapulogalamu a genomic, omwe amadaliridwa kwambiri ndi ofufuza m'mabwalo osiyanasiyana kuphatikiza zamankhwala, zaulimi, zachilengedwe, ndi zina. BMKCloud yadzipereka kupereka ntchito zophatikizika, zodalirika komanso zogwira ntchito kuphatikiza nsanja ndi zida zowunikira bioinformatics. BMKCloud ili ndi zida zosiyanasiyana zomwe zimagwiritsidwa ntchito pafupipafupi monga gene annotation, evolutionary genetic zida, ncRNA, kuwongolera kwamtundu wa data, kusonkhanitsa, kulumikizitsa, kuchotsa deta, masinthidwe, ziwerengero, jenereta, masanjidwe. kusanthula, etc.
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RNA yaying'ono
Ma RNA ang'onoang'ono ndi mtundu wa RNA yaifupi yosalemba zilembo yokhala ndi kutalika kwa 18-30 nt, kuphatikiza miRNA, siRNA ndi piRNA.Ma RNA ang'onoang'ono awa akhala akufotokozedwa mochuluka kuti akugwira nawo ntchito zosiyanasiyana zamoyo monga kuwonongeka kwa mRNA, kulepheretsa kumasulira, kupanga heterochromatin, ndi zina zotero. Sequencing kusanthula nsanja imakhala ndi kusanthula kokhazikika komanso migodi yapa data yapamwamba.Pamaziko a RNA-seq data, kusanthula kokhazikika kumatha kukwaniritsa chizindikiritso ndi kulosera kwa miRNA, kulosera kwamtundu wa miRNA, kusanthula ndi kusanthula mawu.Kusanthula kwapamwamba kumathandizira kusaka ndi kutulutsa kwa miRNA, kupanga zojambula za Venn, miRNA ndi kumanga kwa ma gene network.
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NGS-WGS (Illumina/BGI)
NGS-WGS ndi nsanja yonse yowunikiranso ma genome, yomwe imapangidwa potengera luso lazochita mu Biomarker Technologies.Pulatifomu yosavuta kugwiritsa ntchito iyi imalola kutumizira mwachangu kusanthula kophatikizika kwa kachitidwe pongoyika magawo angapo oyambira, omwe amakwanira ma data a DNA opangidwa kuchokera ku nsanja ya Illumina ndi nsanja yotsatirira ya BGI.Pulatifomuyi imayikidwa pa seva yapakompyuta yogwira ntchito kwambiri, yomwe imapatsa mphamvu kusanthula kwachangu kwa data munthawi yochepa kwambiri.Kutsatsa kwamunthu payekha kumapezeka potengera kusanthula kokhazikika, kuphatikiza funso losinthika la jini, mapangidwe a PCR primer, ndi zina zambiri.
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mRNA(Reference)
Transcriptome ndi kulumikizana pakati pa chidziwitso cha ma genetic ndi proteome ya biological function.Kuwongolera mulingo wa transcriptal ndiye njira yofunikira kwambiri komanso yophunziridwa kwambiri pazamoyo.Kutsatizana kwa Transcriptome kumatha kutsatizana zolembedwazo nthawi iliyonse kapena munthawi iliyonse, ndi chigamulo cholondola ku nucleotide imodzi. Imatha kuwonetsa mozama kuchuluka kwa kulembedwa kwa jini, kuzindikira nthawi yomweyo ndikuwerengera zolembedwa zosawerengeka komanso zachilendo, ndikupereka chidziwitso chokhazikika cha zitsanzo zolembedwa zenizeni.
Pakalipano, teknoloji yotsatizana ya transcriptome yakhala ikugwiritsidwa ntchito kwambiri muzofukufuku, zamankhwala ndi zina zofufuza, kuphatikizapo malamulo okhudza chitukuko cha zinyama ndi zomera, kusintha kwa chilengedwe, kugwirizana kwa chitetezo cha mthupi, kusintha kwa jini, kusinthika kwamtundu wamtundu ndi chotupa ndi matenda a chibadwa.
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Metagenomics (NGS)
Pulatifomu yowunikirayi idapangidwa kuti iwunikenso data ya shotgun metagenomic pazaka zazaka zambiri.Zili ndi kayendetsedwe ka ntchito kophatikizana komwe kumakhala ndi kusanthula kwamitundu yosiyanasiyana komwe kumafunikira kwambiri kuphatikiza kusanthula kwa data, maphunziro amtundu wamitundu, maphunziro amtundu wa jini, binning ya metagenome, ndi zina zambiri. , kuyika magawo, kupanga kwamunthu payekha, ndi zina.
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LncRNA
Ma RNA aatali osalemba zilembo (lncRNA) ndi mtundu wa zolembedwa zotalika kuposa 200 nt, zomwe sizitha kulemba ma protein.Umboni wochuluka umasonyeza kuti ma lncRNA ambiri amatha kugwira ntchito.Ukadaulo wotsogola wotsogola kwambiri ndi zida zowunikira za bioinformatic zimatipatsa mphamvu kuti tiwulule masanjidwe a lncRNA ndikuyika zidziwitso moyenera komanso kutitsogolera kuti tipeze ma lncRNA okhala ndi ntchito zofunikira zowongolera.BMKCloud imanyadira kupatsa makasitomala athu lncRNA nsanja yowunikira kuti akwaniritse kusanthula kwachangu, kodalirika komanso kosinthika kwa lncRNA.
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GWAS
Genome-wide association study (GWAS) cholinga chake ndi kuzindikira mitundu yosiyanasiyana ya majini (genotype) yomwe imakhudzana ndi makhalidwe enaake (phenotype).Kafukufuku wa GWA amafufuza zolembera zamtundu wamtundu wonse wa anthu ambiri ndikulosera mayanjano a genotype-phenotype posanthula kuchuluka kwa anthu.Kubwereza kwamtundu wonse kumatha kupeza mitundu yonse ya ma genetic.Kuphatikizana ndi deta ya phenotypic, GWAS ikhoza kukonzedwa kuti izindikire ma SNP okhudzana ndi phenotype, ma QTL ndi majini osankhidwa, omwe amathandizira kwambiri kuswana nyama / zomera zamakono.SLAF ndi njira yodzipangira yokha yosavuta yotsatirira ma genome, yomwe imapeza zolembera zamtundu uliwonse, SNP.Ma SNP awa, monga ma genetic markers, amatha kusinthidwa kuti agwirizane ndi zomwe akutsata.Ndi njira yotsika mtengo pozindikira zovuta zomwe zimakhudzidwa ndi kusiyanasiyana kwa ma genetic.
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Nanopore Full-length transcriptomics
Ma isoform ovuta komanso osinthika m'zamoyo ndi njira zofunika kwambiri zoyendetsera ma jini komanso kusiyanasiyana kwa mapuloteni.Kuzindikiritsa kolondola kwa zolembedwa ndi maziko owerengera mozama machitidwe owongolera ma jini.Nanopore sequencing platform yabweretsa bwino transcriptomic study ku isoform-level.Pulatifomu yowunikirayi idapangidwa kuti iwunikenso data ya RNA-Seq yopangidwa pa nsanja ya Nanopore pamaziko a genome, yomwe imakwaniritsa kusanthula kwamtundu komanso kuchuluka kwamitundu yonse komanso mulingo wa zolembedwa.
