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BMKMANU S1000 Spatial Transcriptome

Kulinganiza kwa malo kwa maselo kumagwira ntchito yofunika kwambiri pazochitika zosiyanasiyana zamoyo, monga kulowetsedwa kwa chitetezo cha mthupi, kukula kwa mwana wosabadwayo, ndi zina zotero. Kutsatizana kwa malo, komwe kumasonyeza mbiri ya jini ndikusunga zambiri za malo, kwapereka chidziwitso chambiri mu kamangidwe ka minofu ya transcriptome.Ndi chitukuko chaukadaulo, mawonekedwe owoneka bwino a minofu ndi kusiyana kwenikweni kwamawonekedwe amtundu wa ma cell amayenera kuphunziridwa bwino kwambiri.BMKGENE imapereka ntchito yokwanira, yoyimitsa malo amodzi kuchokera ku zitsanzo kupita ku chidziwitso chachilengedwe.

Matekinoloje a Spatial transcriptomics adapatsa mphamvu malingaliro atsopano m'mabwalo osiyanasiyana ofufuza pothetsa mbiri ya jini yokhala ndi zopezeka m'magawo osiyanasiyana.

Spatial transcriptome chip: BMKMANU S1000

Pulatifomu: Illumina NovaSeq Platform


Tsatanetsatane wa Utumiki

Bioinformatics

Zotsatira za Demo

BMKMANU S1000 Spatial Transcriptome Technical Scheme

S1000.

Ubwino wa BMKMANU S1000

1) Kusintha kwa ma cell ang'onoang'ono: Malo aliwonse ojambulidwa amakhala ndi >2 miliyoni Mabala A Barcode okhala ndi mainchesi a 2.5 µm ndi motalikirana wa 5 µm pakati pa malo omwe ali ndi malo, zomwe zimathandiza kusanthula kwa malo okhala ndi ma sub-cellular resolution (5 µm).

s1000 (1)

2) Kusanthula kwamasinthidwe angapo: Kusanthula kosinthika kwamitundu ingapo kuyambira 100 μm mpaka 5 μm kuti athetse mitundu yosiyanasiyana ya minofu pakuwongolera koyenera.

s1000 (2)

3) Kufotokozera kwatsatanetsatane: Zolemba zomwe zatengedwa kuchokera pazithunzi zonse zimatha kufufuzidwa, popanda kuletsa kuchuluka kwa majini omwe mukufuna komanso malo omwe mukufuna.

s1000 (3)

Mafotokozedwe a Utumiki

Library

Njira yotsatirira

Data Output tikulimbikitsidwa

S1000 cDNA library

BMKMANU S1000-Illumina PE150

60Gb / chitsanzo

Zitsanzo Zofunika

Chitsanzo

Nambala

Kukula

Mtengo wa RNA

OCT yophatikizidwa ndi minofu

2-3 midadada / chitsanzo

Pafupifupi.6.8x6.8x6.8 mm3

RIN≥7

Kuti mumve zambiri zachitsogozo chokonzekera zitsanzo ndi kayendedwe ka ntchito, chonde omasuka kulankhula ndi a

Kuyenda kwa Ntchito Yantchito

Chitsanzo cha QC

Kuyesera kupanga

kutumiza chitsanzo

Kupereka zitsanzo

Kuyesera koyendetsa ndege

Yesetsanitu

Kuyika barcode

Kuyika barcode

Kukonzekera kwa Library

Kumanga laibulale

Kutsata

Kutsata

Kusanthula deta

Kusanthula deta

Pambuyo pogulitsa Services

Pambuyo pogulitsa ntchito


  • Zam'mbuyo:
  • Ena:

  • 10x (9)

    Deta yopangidwa ndi BMKMANU S1000 imawunikidwa pogwiritsa ntchito pulogalamu ya "BSTMatrix", yomwe idapangidwa palokha ndi BMKGENE, kuphatikiza:

    1) Gene expression matrix generation
    2) Kukonza zithunzi za HE
    3) N'zogwirizana ndi mtsinje wachitatu chipani mapulogalamu kusanthula
    4) Paintaneti "BSTViewer" imathandizira kupeza zotsatira zowonera pazosankha zosiyanasiyana.

    1.Spot clustering

    s1000 (4)

    2.Kugawa kwamalo

    s1000 (5)s1000 (6)

    Noti: Kusamvanamlingo = 13 (100 µm, kumanzere); 7 (50 µm, kumanja)

    3.Mawu a Marker kuchuluka komwe kumaphatikiza kutentha

    s1000 (7)

    4.Inter-sample data analysis

    s1000 (8)

     

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