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Zogulitsa

Chromatin Immunoprecipitation Sequencing (ChIP-seq)

ChIP-Seq imapereka mbiri ya genome-wide ya DNA zomwe zikufuna kusintha histone, transcript factor, ndi mapuloteni ena okhudzana ndi DNA.Imaphatikiza kusankha kwa chromatin immuno-precipitation (ChIP) kuti ipezenso ma protein-DNA complexes, ndi mphamvu ya m'badwo wotsatira (NGS) pakutsatizana kwapamwamba kwa DNA yobwezeretsedwa.Kuphatikiza apo, chifukwa ma protein-DNA complexes amatengedwa kuchokera ku maselo amoyo, malo omangira amatha kufananizidwa mumitundu yosiyanasiyana ya maselo ndi minofu, kapena pansi pamikhalidwe yosiyana.Mapulogalamuwa amachokera ku malamulo olembedwa mpaka njira zachitukuko kupita ku njira za matenda ndi kupitirira.

Pulatifomu: Illumina NovaSeq Platform


Tsatanetsatane wa Utumiki

Bioinformatics

Zotsatira za Demo

Ubwino wa Utumiki

● Platform: Illumina NovaSeq Platform, PE150

● Mtundu wa laibulale: 100-500bp lowetsani laibulale ya DNA (zimadalira pa kugawidwa kwakukulu)

● Njira yotsatirira: Paired-End 150 bp

● Limbikitsani zotsatira za deta: 6 Gb yaiwisi deta / chitsanzo.

Zitsanzo Zofunika

Kuchuluka kwa DNA: ≥ 70 ng, nsonga yayikulu ya 100 mpaka 500 bp

Voliyumu yachitsanzo: ≥ 10 μl

OD260/280 = 1.8 mpaka 2.0 popanda kuwonongeka kapena kuipitsidwa kwa RNA

Kuyenda koyeserera

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Kuyenda kwa Ntchito Yantchito

kutumiza chitsanzo

Kupereka zitsanzo

Kukonzekera kwa Library

Kumanga laibulale

Kutsata

Kutsata

Kusanthula deta

Kusanthula deta

Pambuyo pogulitsa Services

Pambuyo pogulitsa ntchito


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    1. Kugawa kwakukulu kwabwino

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    2. MA chiwembu chapamwamba chosiyana

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    3.Chithunzi cha KEGG

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    4. GO Gulu

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