Zogulitsa

Illumina ndi BGI

Tekinoloje yotsatizana ya Illumina, yozikidwa pa Sequencing by Synthesis (SBS), ndiukadaulo wapadziko lonse wa NGS, womwe umapangitsa kuti pakhale 90% yazotsatizana zapadziko lonse lapansi.Mfundo ya SBS imaphatikizapo kujambula zoziziritsa zotchedwa fulorosenti zotchedwa reversible terminators pamene dNTP iliyonse imawonjezedwa, ndipo kenako imadulidwa kuti ilole kuphatikizidwa kwa maziko otsatirawa.Ndi ma dNTP onse anayi osinthika omangika otsekera omwe amapezeka mumayendedwe aliwonse, mpikisano wachilengedwe umachepetsa kukondera.Ukadaulo wosunthikawu umathandizira malaibulale owerengeka okha komanso ophatikizidwa awiriawiri, popereka ma genomic applications.Illumina sequencing imatengera luso lapamwamba kwambiri komanso kulondola kwake, imayiyika ngati mwala wapangodya pa kafukufuku wa genomics, kupatsa mphamvu asayansi kuti avumbulutse zovuta za ma genomes mwatsatanetsatane komanso mosayerekezeka.

DNBSEQ, yopangidwa ndi BGI, ndiukadaulo winanso waukadaulo wa NGS womwe wakwanitsa kutsitsa mtengo wotsatizana ndikuwonjezera zotuluka.Kukonzekera kwa malaibulale a DNBSEQ kumaphatikizapo kugawikana kwa DNA, kukonzekera ssDNA ndi kukweza kozungulira kuti mupeze ma nanoballs a DNA (DNB).Izi zimayikidwa pamalo olimba ndikutsatiridwa ndi combinatorial Probe-Anchor Synthesis (cPAS).

Ntchito yathu yotsatirira laibulale yomwe idapangidwa kale imathandizira makasitomala kukonzekera malaibulale awo otsatizana kuchokera kumagwero osiyanasiyana (mRNA, genome yonse, amplicon, pakati pa ena).Pambuyo pake, malaibulalewa atha kutumizidwa kumalo athu otsatirira kuti azitha kuyang'anira bwino komanso kutsatizana pamapulatifomu a Illumina kapena BGI.

Lumikizanani nafe

Tsatanetsatane wa Utumiki

Zotsatira za Demo

Mawonekedwe

●Mapulatifomu:Illumina NovaSeq 6000, NovaSeq , HiSeq X Ten ndi BGI-DNB-T7

●Masanjidwe:PE50, PE100, PE150, PE250

●Kuwongolera kwabwino kwama library musanayambe kutsatizana

●Kutsata kutumizidwa kwa data ndi QC:kubweretsa lipoti la QC ndi data yaiwisi mumtundu wa fastq mutatha demultiplexing ndikusefa Q30 yowerengedwa.

Ubwino wa Utumiki

●Kusiyanasiyana kwa Sequencing Services:kasitomala angasankhe motsatana ndi kanjira, otaya selo kapena kuchuluka kwa deta.

●Kusiyanasiyana kwamapulatifomu:Ma library a DNB amatha kusamutsidwa ku nsanja za Illumina

●Zochitika zambiri pa nsanja ya Illumina:ndi masauzande ambiri otsekedwa ndi mitundu yosiyanasiyana.

●Kutumiza kwa sequencing lipoti la QC:ndi ma metrics abwino, kulondola kwa data komanso magwiridwe antchito onse a projekiti yotsatizana.

●Njira yotsatirira anthu okhwima:ndi nthawi yochepa yozungulira.

●Kuwongolera Kwabwino Kwambiri: timakhazikitsa zofunikira za QC kuti zitsimikizire kuperekedwa kwa zotsatira zapamwamba nthawi zonse.

Zitsanzo Zofunika *

Kutsatizana Mwapang'ono

Mtengo wa data (X)	Kukhazikika (qPCR/nM)	Voliyumu
X ≤ 50 Gb	≥ 2 nM	≥ 20 μl
50 Gb ≤ X <100 Gb	≥3 nM	≥ 20 μl
X ≥ 100 Gb	≥ 4 nM	≥ 20 μl

Single Lane (Illumina)

nsanja	Kukhazikika (qPCR/nM)	Voliyumu
HiSeq X Ten	≥ 2 nM	≥ 20 μl
NovaSeq 6000 SP	≥1 nM	≥ 25 μl
NovaSeq 6000 S4	≥ 1.5 nM	≥ 25 μl
NovaSeq X	≥ 1.5 nM	≥ 25 μl
BGI-DNBSEQ-T7	≥ 1.5 nM	≥ 25 μl

Kuphatikiza pa kukhazikika komanso kuchuluka kwake, pafunikanso patali yoyenera.

Chonde tithandizeni ngati zitsanzo zanu sizikukwaniritsa zofunikira zoyambira.

Lumikizanani nafe

Njira ya utumiki

Kuwongolera khalidwe la library

Kutsata

Kuwongolera khalidwe la deta

Kutumiza kwa polojekiti

Zam'mbuyo: DNA / RNA sequencing -PacBio Sequencer

Ena: DNA / RNA Sequencing - Nanopore Sequencer

Library QC lipoti

Lipoti laubwino wa laibulale imaperekedwa musanasande, kuwunika kuchuluka kwa laibulale, ndi kugawikana.

Kutsata lipoti la QC

Gulu 1. Ziwerengero za kusanja deta.

Chitsanzo cha ID	BMKID	Yaiwisi amawerenga	Raw Data (bp)	Zowerenga zoyera (%)	Q20(%)	Q30(%)	GC(%)
C_01	BMK_01	22,870,120	6,861,036,000	96.48	99.14	94.85	36.67
C_02	BMK_02	14,717,867	4,415,360,100	96.00	98.95	93.89	37.08