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DNA / RNA Sequening - nanopore Seersiter

DNA / RNA Sequening - Nanopore Seercer Wolemba Chithunzi

Kudula kwa ONT ndi njira imodzi yamagetsi yamagetsi yozungulira potengera ma nanopores, mfundo zotsekerera zapulatifomu iliyonse ndizofanana. Wosuntha wowongoka kawiri muyeso. Chifukwa chakusiyana kwa mankhwala a mabasi osiyanasiyana pa DNA / RNA Squand, pomwe molekyulu amodzi amadutsa njira ya nanomore, imasintha magetsi osiyanasiyana. Mwa kuzindikira ndikugwirizana ndi zizindikilo izi, mitundu yofananira imatha kuwerengedwa, ndipo kupezeka kwenikweni kwa nthawiyo kumatha.

Lumikizanani nafe

Zambiri

Zotsatira za Demo

Zambiri za ntchito

Nsanja	Kukula kwa library	Zokolola zam'madzi (pa selo)	Kulondola kwa maziko amodzi	Mapulogalamu
Nanomore	8kb, 10kb, 20kb, Umerlong, CDNA-PCR	70-90GB / cell	85-92%	SV imayimba, de Novo, Seat-Tizingle-Utumbo Wamtali, Iso-Seq, Gene, kuzindikiritsa DNA Methylation

Ubwino Wantchito

● Zomwe zakadutsa zaka zopitilira 5 papulatifomu yosindikizira pacbio yokhala ndi ma projekiti atsekedwa ndi mitundu yosiyanasiyana.
● BMKGene ndi mnzake wovomerezeka wa oxford nanopore, ndi gawo lambiri RNA / DNA.
● Pali mitundu yoyambira yotsatira ndi zida zokwanira komanso zigawo zokwanira.
● Kutengera pa nsanja ya nanopore, zopitilira 10 ndi zobzala denovo zasindikizidwa m'dziko lodziwika padziko lonse lapansi.

Zofunikira Zitsanzo

Mtundu wa zitsanzo

Ndalama

Hidrtion (Quit ®)

Phokoso

Kukhala Uliri

Ena

Gen DNA

Zimatengera zofunikira za deta

≥20ng / μl

≥15μL

Ode260 / 280 = 1.7-2.2;

Ode260 / 230≥1.5;

Chomveka chachikulu pa 260 nm, palibe chidetso

Zovuta zimafunikira kuyesedwa ndi Quibit ndi Quit / Nanopore ≤ 2

RNA yonse

≥1.2μg

≥100μg / μl

≥15μL

Ode260 / 280 = 1.7-2.5;

Ode260 / 230 = 0.5-2.5; palibe zodetsedwa

RIN Mtengo ≥7.5

Ntchito Yogwira Ntchito

Kukonzekera zitsanzo

Kukonzekera zitsanzo

Kukonzekera la laibule

Ntchito Yomanga Laibulale

Kusazidza

Kusazidza

Kusanthula kwa data

Kusanthula kwa data

Zitsanzo QC

Kutumiza kwa Project

M'mbuyomu: Audina Yoyambirira

Ena: Chingwe Chachikulu cha Chuma Chachikulu (Slaf-Seq)

Kuyeserera kwa data kwa DNA

Gome 1. Ziwerengero pa data yoyera.

Bmkid	rawseqnum	rawsuse	kuyeretsa	kuyeretsa	woyera	oyera	emoni	kuyerekezeraxn	solaansqualqual
DNA_bmk01	1,218,239	26.37	1,1211,736	25.90	28,014	15,764	23,090	143,181	9

Kuyeserera kwa data kwa RNA

Gome 1. Ziwerengero pa data yoyera.

Dzina lafayilo	Kazimayizi	Kuwerenga	Balanjim	N50	Maloto	Matala	Tanthauzo
Rna_bmk001	C2	8,947,708	4,047,230,083	398	452	129,227	Q12

Chithunzi 1. Werengani magawidwe ogawa kutalika

Chithunzi 2.

Chithunzi 3. Kutalika ndi Kugawa Kwambiri Kwambiri

Pezani mawu

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