Lowani mu BMKCloud

Zogulitsa

Kutsata kwa DNA/RNA - Nanopore Sequencer

Kutsata kwa ONT ndi ukadaulo umodzi wotsata zizindikiro zamagetsi nthawi yeniyeni kutengera ma nanopores, mfundo yotsatizana ya nsanja iliyonse ndi yofanana. DNA/RNA yokhala ndi zingwe ziwiri imalumikizana ndi mapuloteni a nanoporous omwe ali mu biofilm ndikumasuka pansi pa utsogoleri wa protein ya mota, pansi pa kusiyana kwa magetsi kuchokera mbali zonse ziwiri za biofilm, zingwe za DNA/RNA zimadutsa mu protein ya nanopore pamlingo winawake. Chifukwa cha kusiyana kwa mankhwala a maziko osiyanasiyana pa chingwe cha DNA/RNA, pamene maziko amodzi kapena molekyulu ya DNA imadutsa mu nanopore, imayambitsa kusintha kwa zizindikiro zamagetsi zosiyanasiyana. Pozindikira ndikugwirizana ndi zizindikirozi, mitundu yofanana ya maziko imatha kuwerengedwa, ndipo kuzindikira nthawi yeniyeni kwa mndandanda kumatha kumalizidwa.

Lumikizanani nafe

Tsatanetsatane wa Utumiki

Zotsatira za Chiwonetsero

Zambiri za Utumiki

Nsanja	Kukula kwa Laibulale	Kuchuluka kwa Deta Yongopeka (Pa Selo Iliyonse)	Kulondola Kokhazikika Pamodzi	Mapulogalamu
Nanopore	8Kb, 10kb, 20kb, Ultralong, cDNA-PCR	70-90Gb/Selo	85-92%	Kuyimba kwa SV, De novo, Kutsatana kwautali wonse, Iso-Seq, Kufotokozera za Gene, Kuzindikira methylation ya DNA

Ubwino wa Utumiki

● Zaka zoposa 5 za ntchito yanga pa nsanja ya PacBio yotsatizana ndi mapulojekiti ambirimbiri otsekedwa okhala ndi mitundu yosiyanasiyana ya zinthu.
● BMKGENE ndi mnzawo wovomerezeka wa Oxford Nanopore, wokhala ndi satifiketi ya RNA/DNA ya nsanja ziwiri.
● Pali mitundu yodziwika bwino ya ma sequencers okhala ndi zida zonse komanso njira yokwanira yotsatirira.
● Kutengera ndi nsanja ya Nanopore, kafukufuku wopitilira 10 wa zinyama ndi zomera ku Denovo wafalitsidwa m'magazini otchuka padziko lonse lapansi.

Zofunikira pa Zitsanzo

Mtundu wa Chitsanzo

Ndalama

Kukhazikika (Qubit ®)

Voliyumu

Chiyero

Ena

DNA ya majini

Kudalira pa Kufunika kwa Deta

≥20ng/μl

≥15μl

OD260/280=1.7-2.2;

OD260/230≥1.5；

Chotsani chivundikirocho pa 260 nm, palibe kuipitsidwa

Kuchuluka kwa madzi kuyenera kuyezedwa ndi Qubit ndi Qubit/Nanopore ≤ 2

RNA yonse

≥1.2μg

≥100μg/μl

≥15μl

OD260/280=1.7-2.5;

OD260/230=0.5-2.5； palibe kuipitsidwa

Mtengo wa RIN ≥7.5

Kayendedwe ka Ntchito

Kukonzekera chitsanzo

Kumanga laibulale

Kutsatana

Kusanthula deta

Kupereka ntchito

Yapitayi: Malaibulale opangidwa kale a Illumina

Ena: Kutsatana kwa Zidutswa Zodziwika Bwino (SLAF-Seq)

Kuwunika Ubwino wa Deta ya Chitsanzo cha DNA

Gome 1. Ziwerengero pa deta yoyera.

BMKID	rawSeqNum	rawSumBase	cleanSeqNum	cleanSumBase	cleanN50Len	cleanN90Len	cleanMeanLen	cleanMaxLen	cleanMeanQual
DNA_BMK01	1,218,239	26.37	1,121,736	25.90	28,014	15,764	23,090	143,181	9