Zogulitsa

DNA / RNA Sequencing - Nanopore Sequencer

Kutsatizana kwa ONT ndi molekyu imodzi yeniyeni yotsatizana ndi teknoloji yotsatizana ndi nanopores, ndondomeko yotsatizana ya nsanja iliyonse ndi yofanana.DNA/RNA yokhala ndi mizere iwiri imamanga ku mapuloteni a nanoporous ophatikizidwa mu biofilm ndikusungunula motsogozedwa ndi mapuloteni amoto, pansi pa kusintha kwa magetsi kuchokera mbali zonse za biofilm, zingwe za DNA/RNA zimadutsa mu protein ya nanopore panthawi inayake. mlingo.Chifukwa cha kusiyana kwa mankhwala azitsulo zosiyanasiyana pa DNA / RNA strand, pamene maziko amodzi kapena molekyulu ya DNA imadutsa mumsewu wa nanopore, zidzachititsa kusintha kwa zizindikiro zosiyanasiyana zamagetsi.Pozindikira ndi kufananiza ndi zizindikirozi, mitundu yofananira yoyambira imatha kuwerengedwa, ndipo kuzindikira nthawi yeniyeni yotsatizana kumatha kutha.

Lumikizanani nafe

Tsatanetsatane wa Utumiki

Zotsatira za Demo

Tsatanetsatane wa Utumiki Features

nsanja	Kukula kwa Library	Theoretical Data Yield (Per Cell)	Kulondola Pamodzi	Mapulogalamu
Nanopore	8Kb, 10kb, 20kb, Ultralong, cDNA-PCR	70-90Gb / Cell	85-92%	Kuitana kwa SV, De novo, Kutsatizana kwautali, Iso-Seq, ndemanga ya Gene, Kuzindikira kwa DNA methylation

Ubwino wa Utumiki

● Kupitilira zaka 5 papulatifomu yotsatizana ya PacBio yokhala ndi mapulojekiti masauzande ambiri otsekedwa okhala ndi mitundu yosiyanasiyana.
● BMKGENE ndi mnzawo wovomerezeka wa Oxford Nanopore, wokhala ndi ziphaso zapawiri za RNA/DNA.
● Pali mitundu yambiri ya sequencers yokhala ndi zida zonse ndi ndondomeko yokwanira yotsatizana.
● Malingana ndi nsanja ya Nanopore, kafukufuku woposa 10 wa zinyama ndi zomera za Denovo zasindikizidwa m'magazini otchuka padziko lonse lapansi.

Zitsanzo Zofunika

Mtundu Wachitsanzo

Ndalama

Concentrtion(Qubit ®)

Voliyumu

Chiyero

Ena

Genomic DNA

Zimatengera Zofunikira pa Data

≥20ng/μl

≥15μl

OD260/280=1.7-2.2;

OD260/230≥1.5;

Chotsani pachimake pa 260 nm, palibe kuipitsidwa

Kukhazikika kuyenera kuyezedwa ndi Qubit ndi Qubit/Nanopore ≤2

Mtengo wa RNA

≥1.2μg

≥100μg/μl

≥15μl

OD260/280=1.7-2.5;

OD260/230=0.5-2.5;palibe zoipitsidwa

Mtengo wa RIN ≥7.5

Njira ya utumiki

Kukonzekera kwachitsanzo

Kumanga laibulale

Kutsata

Kusanthula deta

Kutumiza kwa polojekiti

Zam'mbuyo: DNA / RNA Sequencing - Illumina Sequencer

Ena: Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)

Kuwunika Kwamtundu wa Deta wa Zitsanzo za DNA

Table 1. Ziwerengero za deta yoyera.

BMKID	rawSeqNum	rawSumBase	cleanSeqNum	cleanSumBase	cleanN50Len	cleanN90Len	cleanMeanLen	cleanMaxLen	cleanMeanQual
DNA_BMK01	1,218,239	26.37	1,121,736	25.90	28,014	15,764	23,090	143,181	9