Lowani mu BMKCloud

Zogulitsa

Malaibulale opangidwa kale a DNBSEQ

DNBSEQ, yopangidwa ndi MGI, ndi ukadaulo watsopano wa NGS womwe wachepetsa kwambiri mtengo wotsatizana ndi kuonjezera kuchuluka kwa zinthu zomwe zingagwiritsidwe ntchito. Kukonzekera malaibulale a DNBSEQ kumaphatikizapo kugawikana kwa DNA, kukonzekera ssDNA, ndi kukulitsa kuzungulira kuti mupeze ma nanoball a DNA (DNB). Izi zimayikidwa pamalo olimba kenako zimatsatiridwa ndi combinatorial Probe-Anchor Synthesis (cPAS). Ukadaulo wa DNBSEQ umaphatikiza zabwino zokhala ndi chiwopsezo chochepa cha kukulitsa ndi kugwiritsa ntchito njira zolakwitsa zapamwamba kwambiri ndi ma nanoball, zomwe zimapangitsa kuti kutsatizana kukhale ndi kuchuluka kwa zinthu zomwe zingagwiritsidwe ntchito komanso molondola.

Ntchito yathu yokonzekera ma laibulale imalola makasitomala kukonzekera ma laibulale owerengera a Illumina kuchokera kuzinthu zosiyanasiyana (mRNA, genome yonse, amplicon, ma laibulale 10x, pakati pa ena), omwe amasinthidwa kukhala ma laibulale a MGI m'ma laboratories athu kuti atsatidwe mu DNBSEQ-T7, zomwe zimathandiza kuti deta ikhale yambiri pamtengo wotsika.

Lumikizanani nafe

Tsatanetsatane wa Utumiki

Zotsatira za Chiwonetsero

Mawonekedwe

●Nsanja:MGI-DNBSEQ-T7

●Njira zotsatirira:PE150

●Kusamutsa malaibulale a Illumina kupita kuMGI:kulola kutsata kuchuluka kwa deta yambiri pamtengo wotsika.

●Kuwongolera khalidwe la malaibulale musanatsatire malamulo.

●Kutsata QC ya data ndi kutumiza:Kupereka lipoti la QC ndi deta yosaphika mu mtundu wa fastq pambuyo pochotsa zinthu zambiri ndikusefa ma Q30.

Ubwino

●Kusinthasintha kwa ntchito zotsatizana:kasitomala angasankhe kutsata njira ndi njira kapena kuchuluka kwa deta.

●Kutulutsa deta yambiri:1500 Gb/mzere

●Kupereka lipoti la QC lotsatizana:ndi miyeso ya khalidwe, kulondola kwa deta komanso momwe polojekiti yonse yotsatirira zinthu ikuyendera.

●Njira yotsatirira okhwima:ndi nthawi yochepa yobwerera.

●Kulamulira Kwabwino Kwambiri: Timagwiritsa ntchito zofunikira za QC kuti titsimikizire kuti zotsatira zabwino nthawi zonse zimaperekedwa.

Zofunikira pa Zitsanzo

	Kuchuluka kwa Deta (X)	Kuchuluka kwa madzi (qPCR/nM)	Voliyumu
Msewu Wochepa	X ≤ 10 Gb	≥ 1nM	≥ 25 μl
	10 Gb < X ≤ 50 Gb	≥ 2 nM	≥ 25 μl
	50 Gb < X ≤ 100 Gb	≥ 3 nM	≥ 25 μl
	X > 100 Gb	≥ 4 nM
Msewu Waung'ono	Pa Msewu uliwonse	≥ 1.5 nM / Dziwe la laibulale	≥ 25 μl / Dziwe la laibulale

Kuwonjezera pa kuchuluka kwa madzi ndi kuchuluka kwake, njira yoyenera yopezera madzi ndi yofunika.

Zindikirani: Kutsata njira za malaibulale okhala ndi mitundu yosiyanasiyana kumafuna kuti PhiX iwonjezereke kuti zitsimikizire kuti malo oyambira ali olimba.

Tikukulimbikitsani kutumiza malaibulale omwe adayikidwa kale ngati zitsanzo. Ngati mukufuna kuti BMKGENE achite ma laibulale ophatikizana, chonde onani

zofunikira za laibulale pakutsata njira pang'ono.

Kukula kwa Laibulale (Mapu a pamwamba)

Chimake chachikulu chiyenera kukhala mkati mwa 300-450 bp.

Malaibulale ayenera kukhala ndi chitoliro chimodzi chachikulu, osawononga ma adapter komanso opanda ma primer dimers.

Kayendedwe ka Ntchito

Yapitayi: Kuyanjana kwa Chromatin kochokera ku Hi-C

Ena: BMKMANU S3000_Spatial Transcriptome

Lipoti la QC la Library

Lipoti la ubwino wa laibulale limaperekedwa musanayang'ane mndandanda, kuwunika kuchuluka kwa laibulale, ndi kugawikana.

Lipoti la QC lotsatizana

Gome 1. Ziwerengero pa deta yotsatizana.

Chitsanzo cha ID	BMKID	Zowerenga zosaphika	Deta Yosaphika (bp)	Kuwerenga koyera (%)	Q20(%)	Q30(%)	GC(%)
C_01	BMK_01	22,870,120	6,861,036,000	96.48	99.14	94.85	36.67
C_02	BMK_02	14,717,867	4,415,360,100	96.00	98.95	93.89	37.08