DNBSEQ Pre-Creadle
Mawonekedwe
●Pulatifomu:Mgi-dnbseq-t7
●Kumata Modes:Pe150
●Kusamutsa ma risitino aMgi:Kulimbikitsa zingwe za kuchuluka kwa mapangidwe apamwamba pamtengo wotsika.
●Kuwongolera kwa malamulo ovomerezeka musanayambe kubera.
●Zosunga Zambiri QC ndi kutumiza:Kutumiza kwa lipoti la QC ndi tsatalowedwe chosaphika mu Flatq mtundu utatha kufooketsa ndi kusefa Q30.
Ubwino
●Kuthana ndi Ntchito Zoyeserera:Makasitomala amatha kusankha kutsatira njira kapena kuchuluka kwa deta.
●Zotulutsa zapamwamba:1500 GB / Lane
●Kutumiza QC Ilipoti QC:Ndi zitsulo zabwino, kulondola kwa data komanso kugwira ntchito kwambiri polojekiti.
●Njira Yoyeserera Yokhwima:nthawi yokhazikika.
●Chiwongolero cholimba: Timakhazikitsa zofunikira za QC kuti zitsimikizire kuti zopereka zapamwamba kwambiri.
Zofunikira Zitsanzo
| Kuchuluka kwa deta (x) | Kuzindikira (QPCR / NM) | Mabuku | |
| Msewu pang'ono
| X ≤ 10 gb | ≥ 1nm | ≥ 25 μl |
| 10 gb <x ≤ 50 gb | ≥ 2 nm | ≥ 25 μl | |
| 50 gb <x ≤ 100 gb | ≥ 3 nm | ≥ 25 μl | |
| X> 100 GB | ≥ 4 nm | ||
| Msewu Waung'ono | Msewu uliwonse | ≥ 1.5 nm / laibulale | ≥ 25 μl / laibulale |
Kuphatikiza pa chidwi chachikulu komanso kuchuluka kwathunthu, njira yoyenera imafunikiranso.
Chidziwitso: Kudula kwamilandu kumayiko osiyanasiyana kumafunikira PHIX Spike-potsimikizira kuyitanidwa.
Timalimbikitsa kutumiza malaibulale okhazikika ngati zitsanzo. Ngati mukufuna BMKGEne kuti mupange pooling, chonde onani
Zofunikira za library ya sequine yozungulira pang'ono.
Kukula kwa Library (Map Peak)
Chikondwerero chachikulu chizikhala mkati mwa 300-50 bP.
Malaibulale ayenera kukhala ndi nsonga imodzi yayikulu, osadetsa kutsatsa ndipo palibe wopondapo.
Ntchito Yogwira Ntchito
Library QC Lipoti la
Lipoti la mtundu wa laibulale limaperekedwa asanasindikize, kuwunika kuchuluka kwa library, ndi kugawanika.
Sefening QC Ipotilo
Gome 1. Zolemba pa deta yotsekemera.
| ID yachitsanzo | Bmkid | Waiwisi amawerenga | Data yaiwisi (BP) | Oyera amawerengera (%) | Q20 (%) | Q30 (%) | GC (%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Chithunzi 1. Kugawa koyenera kumawerengedwa mu chitsanzo chilichonse
Chithunzi 2. Kugawidwa Kwambiri
Chithunzi 3. Kugawa kwa zomwe zalembedwazi mu data
